Results 131 to 140 of about 122,125 (182)

Comparison of gene transcription in cloned bovine embryos produced by different nuclear transfer techniques

Molecular Reproduction and Development, 2001
AbstractThe efficiency of animal production using cloning technology is still relatively low and research to determine a more efficient nuclear transfer procedure is ongoing. One approach which may be informative in assessing the viability of nuclear transfer embryos is the analysis of embryonic gene expression.
Robert Daniels, Vanessa Jane Hall, A. J. French, Natasha A. Korfiatis, Alan Trounson   +4 more
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Characterization of calcium oscillation patterns in caprine oocytes induced by IVF or an activation technique used in nuclear transfer

Theriogenology, 2005
Routine activation of nuclear transfer (NT) eggs involves the application of a single intracellular calcium [Ca2+]i rise, stimulated by an electrical pulse, as opposed to [Ca2+]i oscillations, which is the natural mode of sperm-induced activation at fertilization in all mammalian species tested to date.
Teru Jellerette, D. Melican, Robin Butler, S. Nims, Carol A. Ziomek, Rafael A. Fissore, W. Gavin   +6 more
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Assessment of nuclear transfer techniques to prevent the transmission of heritable mitochondrial disorders without compromising embryonic development competence in mice

Mitochondrion, 2014
To evaluate and compare mitochondrial DNA (mtDNA) carry-over and embryonic development potential between different nuclear transfer techniques we performed germinal vesicle nuclear transfer (GV NT), metaphase-II spindle-chromosome-complex (MII-SCC) transfer and pronuclear transfer (PNT) in mice.
Jitesh Neupane, Mado Vandewoestyne, Sabitri Ghimire, Yuechao Lu, Chen Qian, Rudy Van Coster, Jan Gerris, Tom Deroo, Dieter Deforce, Petra De Sutter, Björn Heindryckx   +10 more
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Editing the CYP19 Gene in Goat Embryos Using CRISPR/Cas9 and Somatic Cell Nuclear Transfer Techniques

Cellular Reprogramming
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR/Cas9) system is revolutionizing genome engineering and is expected to bring significant advancements in livestock traits, including the treatment of genetic diseases. This study focuses on CRISPR/Cas9-mediated modifications of the CYP19 gene, which encodes aromatase, an enzyme ...
Ahmad Pirali, Farnoosh Jafarpour, Mehdi Hajian, Seyed Hossein Hosseini Moghaddam, Reza Moradi, Nima Tanhaie-vash, Mohsen Rahimi Andani, Tayebeh Izadi, Hanieh Shiralian-Esfahani, Zahra Safaeinejad, Wilfried A. Kues, Mohammad Hossein Nasr‐Esfahani, Shahin Eghbalsaied   +12 more
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Microinsemination, nuclear transfer, and cytoplasmic transfer: the application of new reproductive engineering techniques to mouse genetics

Mammalian Genome, 2001
Atsuo Ogura, Narumi Ogonuki, Kaoru Takano, Kimiko Inoue   +3 more
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Modifications of chemically induced-enucleated nuclear transfer technique by reverse-order nuclear transfer in mouse

Zygote, 2009
SummaryTo improve the developmental potential of somatic cell cloned embryos derived from demecolcine (DC) induced-enucleated nuclear transfer (INT), we modified the INT procedures by transferring donor nuclei into recipient cytoplasts prior to the induced enucleation of the recipient cytoplasts, and we called this modified INT technique as reverse ...
Yong Zhang, Tuo Zhang, Jun Liu, Fengjun Liu, Xiangbin Ding, Zelei Cao, Shuang Tang, Zhilin Guo, Yongsheng Wang, Zhixing An   +9 more
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MEASURING THE HEAT TRANSFER PERFORMANCE OF NUCLEAR REACTOR FUEL ELEMENT CLUSTERS USING- THE TECHNIQUE OF SUPERPOSITION OF TEMPERATURE DISTRIBUTIONS

Proceeding of International Heat Transfer Conference 4, 1970
P.L. Mantle, A. G. Morss
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Nuclear transfer techniques for preimplantation diagnosis and prospect for artificial gamete formation

, 2014

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Evaluation of Lumped Parameter Heat Transfer Techniques for Nuclear Reactor Applications

Nuclear Science and Engineering, 1973
An analytical study was performed to investigate the utility and accuracy of the lumped parameter approach to heat transfer analysis in a nuclear reactor subassembly.
D. M. France, T. Ginsberg
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Significant improvement of mouse cloning technique by treatment with trichostatin A after somatic nuclear transfer

Biochemical and Biophysical Research Communications, 2006
The low success rate of animal cloning by somatic cell nuclear transfer (SCNT) is believed to be associated with epigenetic errors including abnormal DNA hypermethylation. Recently, we elucidated by using round spermatids that, after nuclear transfer, treatment of zygotes with trichostatin A (TSA), an inhibitor of histone deacetylase, can remarkably ...
Eiji Mizutani, Nguyen Van Thuan, Teruhiko Wakayama, Satoshi Kishigami, Hong-Thuy Bui, Takafusa Hikichi, Hiroshi Ohta, Sayaka Wakayama   +7 more
openaire   +3 more sources