Results 141 to 150 of about 528,302 (208)
A Hybrid VGG16-ViT Approach With Image Processing Techniques for Improved White Blood Cell Classification and Disease Diagnosis: A Retrospective Study. [PDF]
Health Sci RepAli MS +10 more
europepmc +1 more source
A Complete Picture of Electron-Nuclear Coupling Dynamics in H₂⁺
Biegert J +14 more
europepmc +1 more source
Identifying routes for transferring spin polarization from parahydrogen to protic solvents.
Chem Commun (Camb)Vaneeckhaute E +4 more
europepmc +1 more source
Development of the techniques for nuclear transfer in pigs
Theriogenology, 1999 Nuclear transfer in pigs was developed in the late 1980's. The techniques were based on previous studies in frogs, mice and cattle. Within stage nuclear transfer, pronuclear exchange, was followed by the transfer of nuclei from cleavage stage embryos. While these have resulted in term development, many problems remain.
T. Tao +2 more
openaire +3 more sources
Application of the Zona-Free Manipulation Technique to Porcine Somatic Nuclear Transfer
Cloning and Stem Cells, 2001 The recent demonstration of a successful zona-free manipulation technique for bovine somatic nuclear transfer (NT) that is both simpler and less labor intensive is of considerable benefit to advance the applications of this technology. Here, we describe that this method is also applicable to porcine somatic NT.
Booth, P J +3 more
openaire +6 more sources
A Double Nuclear Transfer Technique for Cloning Pigs
, 2006 The first round of double nuclear transfer (NT) procedure includes the following steps: transfer of somatic cell nuclei into enucleated recipient oocytes, fusion, activation, and culture of reconstructed oocytes. The next day, a second round of NT is performed by removing karyoplasts from 1-d-old NT embryos and transferring them into in vivo-derived ...
Keith H.S. Campbell +2 more
+6 more sources
Techniques for Nuclear Transfer to Mouse Embryonic Stem Cells
, 2006 In this chapter, methods are described that permit the enucleation of mouse embryonic stem (ES) cells and the transfer of donor nuclei to these cells before or after enucleation has taken place. The small size and high nucleus-to-cytoplasm volume ratio of ES cells poses a challenge to their enucleation.
Paul John Verma +1 more
openaire +4 more sources
Development of novel intracytoplasmic sperm injection and somatic cell nuclear transfer techniques for animal reproduction [PDF]
Animal Science Journal, 2010 ABSTRACTAnimal biotechnology has made new biological experiments possible and new discoveries are being made on an almost daily basis. Among these discoveries is a method for directly injecting a spermatozoa or somatic nucleus into an oocyte that has brought a revolution in the world of micromanipulators. Experiments that were unfeasible until now have
Teruhiko Wakayama
openaire +4 more sources
Reproduction, Fertility and Development, 2005 The aim of the present study was to compare the in vitro and in vivo developmental competence of hand-made cloning (HMC) embryos with the conventional nuclear transfer (NT) method using five somatic cell lines and in vitro-fertilised (IVF; control) embryos.
Renee Hodgson +16 more
openaire +4 more sources
Theriogenology, 2003 This study was conducted to improve a porcine somatic cell nuclear transfer (SCNT) technique by optimizing donor cell and recipient oocyte preparations. Adult and fetal fibroblasts, and cumulus and oviduct cells were used as donor cells, and in vivo- and in vitro-matured oocytes were employed as recipient oocytes.
Hye Soo Kim +9 more
openaire +4 more sources