Results 251 to 260 of about 519,067 (307)

Techniques for Nuclear Transfer to Mouse Embryonic Stem Cells

, 2006
In this chapter, methods are described that permit the enucleation of mouse embryonic stem (ES) cells and the transfer of donor nuclei to these cells before or after enucleation has taken place. The small size and high nucleus-to-cytoplasm volume ratio of ES cells poses a challenge to their enucleation.
Paul John Verma, Daniele Jacqueline Pralong   +1 more
openaire   +4 more sources

Improvement of a porcine somatic cell nuclear transfer technique by optimizing donor cell and recipient oocyte preparations

Theriogenology, 2003
This study was conducted to improve a porcine somatic cell nuclear transfer (SCNT) technique by optimizing donor cell and recipient oocyte preparations. Adult and fetal fibroblasts, and cumulus and oviduct cells were used as donor cells, and in vivo- and in vitro-matured oocytes were employed as recipient oocytes.
Hye Soo Kim, Gabsang Lee, Sang-Hwan Hyun, Sung Keun Kang, Byeong Chun Lee, Jeong Mook Lim, Dae-Young Kim, Eunsong Lee, Woo Suk Hwang, So Hyun Lee   +9 more
openaire   +4 more sources

Comparison of two approaches to nuclear transfer in the bovine: hand-made cloning with modifications and the conventional nuclear transfer technique

Reproduction, Fertility and Development, 2005
The aim of the present study was to compare the in vitro and in vivo developmental competence of hand-made cloning (HMC) embryos with the conventional nuclear transfer (NT) method using five somatic cell lines and in vitro-fertilised (IVF; control) embryos.
Renee Hodgson, Michael K. Holland, Michael K. Holland, R. Tayfur Tecirlioglu, R. Tayfur Tecirlioglu, Ian Lewis, Shara Downie, Andrew J. French, Andrew J. French, Melissa A. Cooney, Melissa A. Cooney, Natasha A. Korfiatis, Natasha A. Korfiatis, Nancy T. Ruddock, Nancy T. Ruddock, Gábor Vajta, Alan O Trounson   +16 more
openaire   +4 more sources

Simplification of Bovine Somatic Cell Nuclear Transfer by Application of a Zona-Free Manipulation Technique

Cloning and Stem Cells, 2001
Contemporary nuclear transfer techniques often require the involvement of skilled personnel and extended periods of micromanipulation. Here, we present details of the development of a nuclear transfer technique for somatic cells that is both simpler and faster than traditional methods.
Booth, P J, Tan, S J, Reipurth, R, Holm, P, Callesen, H   +4 more
openaire   +6 more sources

Comparison of gene transcription in cloned bovine embryos produced by different nuclear transfer techniques

Molecular Reproduction and Development, 2001
AbstractThe efficiency of animal production using cloning technology is still relatively low and research to determine a more efficient nuclear transfer procedure is ongoing. One approach which may be informative in assessing the viability of nuclear transfer embryos is the analysis of embryonic gene expression.
Robert D. Daniels, Andrew J. French, Natasha A. Korfiatis, Alan O Trounson, Vanessa Jane Hall   +4 more
openaire   +4 more sources

Characterization of calcium oscillation patterns in caprine oocytes induced by IVF or an activation technique used in nuclear transfer

Theriogenology, 2006
Routine activation of nuclear transfer (NT) eggs involves the application of a single intracellular calcium [Ca2+]i rise, stimulated by an electrical pulse, as opposed to [Ca2+]i oscillations, which is the natural mode of sperm-induced activation at fertilization in all mammalian species tested to date.
David Melican, S. Nims, Rafael A. Fissore, Carol Ziomek, Robin E. Butler, William G. Gavin, Teru Jellerette   +6 more
openaire   +4 more sources

Assessment of nuclear transfer techniques to prevent the transmission of heritable mitochondrial disorders without compromising embryonic development competence in mice

Mitochondrion, 2014
To evaluate and compare mitochondrial DNA (mtDNA) carry-over and embryonic development potential between different nuclear transfer techniques we performed germinal vesicle nuclear transfer (GV NT), metaphase-II spindle-chromosome-complex (MII-SCC) transfer and pronuclear transfer (PNT) in mice.
Björn Heindryckx, Chen Qian, Rudy Van Coster, Jitesh Neupane, Sabitri Ghimire, Mado Vandewoestyne, Yuechao Lu, Petra De Sutter, Dieter Deforce, Tom Deroo, Jan Gerris   +10 more
openaire   +4 more sources

Microinsemination, nuclear transfer, and cytoplasmic transfer: the application of new reproductive engineering techniques to mouse genetics

Mammalian Genome, 2001
Narumi Ogonuki, Kaoru Takano, Kimiko Inoue, Atsuo Ogura   +3 more
openaire   +4 more sources

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Modifications of chemically induced-enucleated nuclear transfer technique by reverse-order nuclear transfer in mouse

Zygote, 2009
SummaryTo improve the developmental potential of somatic cell cloned embryos derived from demecolcine (DC) induced-enucleated nuclear transfer (INT), we modified the INT procedures by transferring donor nuclei into recipient cytoplasts prior to the induced enucleation of the recipient cytoplasts, and we called this modified INT technique as reverse ...
Yong Zhang, Tuo Zhang, Jun Liu, Fengjun Liu, Xiangbin Ding, Zelei Cao, Shuang Tang, Zhilin Guo, Yongsheng Wang, Zhixing An   +9 more
openaire   +3 more sources

MEASURING THE HEAT TRANSFER PERFORMANCE OF NUCLEAR REACTOR FUEL ELEMENT CLUSTERS USING- THE TECHNIQUE OF SUPERPOSITION OF TEMPERATURE DISTRIBUTIONS

Proceeding of International Heat Transfer Conference 4, 1970
P. L. Mantle, A. G. Morss
openaire   +3 more sources