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On the effects of hyperpolarized water-based dissolution on the solute and solvent ¹H NMR spectra of small molecules.

Phys Chem Chem Phys
Aleshin D, Butbul K, Hahamy I, Abergel D, Frydman L.   +4 more
europepmc   +1 more source

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Development of the techniques for nuclear transfer in pigs

Theriogenology, 1999
Nuclear transfer in pigs was developed in the late 1980's. The techniques were based on previous studies in frogs, mice and cattle. Within stage nuclear transfer, pronuclear exchange, was followed by the transfer of nuclei from cleavage stage embryos. While these have resulted in term development, many problems remain.
Randall S Prather, T Tao, Zoltan Macháty   +2 more
exaly   +3 more sources

Comparison of gene transcription in cloned bovine embryos produced by different nuclear transfer techniques

Molecular Reproduction and Development, 2001
AbstractThe efficiency of animal production using cloning technology is still relatively low and research to determine a more efficient nuclear transfer procedure is ongoing. One approach which may be informative in assessing the viability of nuclear transfer embryos is the analysis of embryonic gene expression.
A J French
exaly   +3 more sources

Comparison of intra- and interspecies nuclear transfer techniques in the production of cloned caprine embryos

Small Ruminant Research, 2011
Abstract Among the ARTs that are applied in goat farming industry, reproductive cloning technology in production of cloned goat embryos are foreseen to facilitate the effort of mass goat production in just a short time frame. There are two possible approaches that can be applied to produce cloned embryos, namely intraspecies SCNT (intraSCNT) and ...
Abdullah, Ramli, Kwong, P.J., Khadijah, W.E.W.   +2 more
exaly   +3 more sources

Evaluation of Lumped Parameter Heat Transfer Techniques for Nuclear Reactor Applications

Nuclear Science and Engineering, 1973
An analytical study was performed to investigate the utility and accuracy of the lumped parameter approach to heat transfer analysis in a nuclear reactor subassembly.
D M France, T Ginsberg
exaly   +2 more sources

Assessment of nuclear transfer techniques to prevent the transmission of heritable mitochondrial disorders without compromising embryonic development competence in mice

Mitochondrion, 2014
To evaluate and compare mitochondrial DNA (mtDNA) carry-over and embryonic development potential between different nuclear transfer techniques we performed germinal vesicle nuclear transfer (GV NT), metaphase-II spindle-chromosome-complex (MII-SCC) transfer and pronuclear transfer (PNT) in mice.
Jitesh Neupane, Yuechao Lu, Rudy Van Coster   +2 more
exaly   +3 more sources

Nuclear transfer techniques for preimplantation diagnosis and prospect for artificial gamete formation

2014
exaly   +4 more sources

Potential for Somatic Nuclear Transfer Technology in Domestic Chickens

Avian Biology Research, 2011
The development of mammalian somatic nuclear transfer techniques by Wilmut et al. in 1997 provided a new option for conserving animal genetic resources; however, this technique cannot be applied directly to avian species due to the anatomical and ...
Atsushi Tajima
exaly   +2 more sources

Modifications of chemically induced-enucleated nuclear transfer technique by reverse-order nuclear transfer in mouse

Zygote, 2009
SummaryTo improve the developmental potential of somatic cell cloned embryos derived from demecolcine (DC) induced-enucleated nuclear transfer (INT), we modified the INT procedures by transferring donor nuclei into recipient cytoplasts prior to the induced enucleation of the recipient cytoplasts, and we called this modified INT technique as reverse ...
Yongsheng, Wang, Jun, Liu, Shuang, Tang, Zhixing, An, Zhilin, Guo, Xiangbin, Ding, Fengjun, Liu, Zelei, Cao, Tuo, Zhang, Yong, Zhang   +9 more
openaire   +2 more sources

Comparison of two approaches to nuclear transfer in the bovine: hand-made cloning with modifications and the conventional nuclear transfer technique

Reproduction, Fertility and Development, 2005
The aim of the present study was to compare the in vitro and in vivo developmental competence of hand-made cloning (HMC) embryos with the conventional nuclear transfer (NT) method using five somatic cell lines and in vitro-fertilised (IVF; control) embryos.
Tecirlioglu, R T, Cooney, M A, Lewis, I M, Korfiatis, N A, Hodgson, R, Ruddock, N T, Vajta, Gabor, Downie, S, Trounson, A O, Holland, M K, French, A J   +10 more
openaire   +4 more sources