Results 281 to 290 of about 146,303 (307)

134 Generation of NANOS2 knockout goats using CRISPR/Cas9 and somatic cell nuclear transfer techniques

Reproduction, Fertility and Development, 2019
The NANOS2 gene, encoding an RNA binding protein, is known to play a critical role in the development of germline for all organisms studied to date. The male mice with biallelic NANOS2 knockouts (KOs) are sterile due to apoptosis of prospermatogonia shortly after birth but with morphologically intact seminiferous tubules. Thus, the choice of NANOS2 for
Z. Fan, M. Regouski, Y. Liu, J. Keim, I. Perisse, J. Oatley, I. Polejaeva   +6 more
openaire   +1 more source

Editing the CYP19 Gene in Goat Embryos Using CRISPR/Cas9 and Somatic Cell Nuclear Transfer Techniques

Cellular Reprogramming
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR/Cas9) system is revolutionizing genome engineering and is expected to bring significant advancements in livestock traits, including the treatment of genetic diseases. This study focuses on CRISPR/Cas9-mediated modifications of the CYP19 gene, which encodes aromatase, an enzyme ...
Ahmad Pirali, Farnoosh Jafarpour, Mehdi Hajian, Seyed Hossein Hosseini Moghaddam, Reza Moradi, Nima Tanhaie-vash, Mohsen Rahimi Andani, Tayebeh Izadi, Hanieh Shiralian-Esfahani, Zahra Safaeinejad, Wilfried Kues, Mohammad-Hossein Nasr-Esfahani, Shahin Eghbalsaied   +12 more
openaire   +2 more sources

Comparison of efficiency of in vitro cloned sheep embryo production by conventional somatic cell nuclear transfer and handmade cloning technique

Reproduction in Domestic Animals, 2018
ContentsConventional somatic cell nuclear transfer (SCNT) technique of in vitro production of cloned embryos involves use of costly and complicated micromanipulators. Handmade cloning (HMC) technique has been applied as efficient and cost‐effective alternative in many livestock species.
S Khan, M Tali, A Khan, S Bhat, A Ashraf, MH Bhat, F Khan, RA Shah   +7 more
openaire   +2 more sources

A novel gene-transfer technique mediated by HVJ (Sendai virus), nuclear protein, and liposomes.

Cancer detection and prevention, 1995
There have been many reports of in vivo direct gene transfer methods, but there are problems with these methods. Recently, we developed a novel, nontoxic and efficient in vivo direct gene transfer method mediated by Sendai virus (HVJ) and liposomes. In our system, foreign genes and nuclear proteins were encapsulated into the same liposomes, which were ...
N, Tomita, J, Higaki, T, Ogihara, T, Kondo, Y, Kaneda   +4 more
openaire   +1 more source

Development of somatic cell nuclear transfer technique for biomedical and agricultural research (year II) : report

2005
There are two objectives of the somatic nuclear transfer studies. The first objective was to evaluate the possibility to produce the inter-species cloned embryos from donor cells of different animals transfer to rabbit, pig or swamp buffalo oocytes. The second objective was to study the donor cell type can significantly affect the in vitro development ...
Mongkol Techakumphu, Pranee Numchaisrika, Ampika Thongpakdee, Rattajuk Rungsiwiwat, Kamtorn Prugsananon, Pramuan Virutamasen   +5 more
openaire   +1 more source

Microinsemination, nuclear transfer, and cytoplasmic transfer: the application of new reproductive engineering techniques to mouse genetics

Mammalian Genome, 2001
A, Ogura, N, Ogonuki, K, Takano, K, Inoue   +3 more
openaire   +2 more sources

A promising nuclear transfer technique for repairing aged oocytes

Fertility and Sterility, 2010
A. Tanaka, M. Nagayoshi, S. Awata, I. Tanaka, H. Kusunoki, S. Watanabe   +5 more
openaire   +1 more source

Transfer Learning Technique Using Similar Phenomena Training for Fuel Digital Twins of Nuclear Systems

13th Nuclear Plant Instrumentation, Control & Human-Machine Interface Technologies (NPIC&HMIT 2023), 2023
James Daniell, Kazuma Kobayashi, Palash Bhowmik, Souvik Chakraborty, Syed Alam   +4 more
openaire   +1 more source

Assessment of Gene Transfer: Magnetic Resonance Imaging and Nuclear Medicine Techniques

2008
Annette Altmann, Uwe Haberkorn
openaire   +1 more source

Somatic cell nuclear transfer: Past, present and future perspectives

Theriogenology, 2007
K H S Campbell
exaly