Results 271 to 280 of about 146,303 (307)
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Application of the Zona-Free Manipulation Technique to Porcine Somatic Nuclear Transfer
Cloning and Stem Cells, 2001The recent demonstration of a successful zona-free manipulation technique for bovine somatic nuclear transfer (NT) that is both simpler and less labor intensive is of considerable benefit to advance the applications of this technology. Here, we describe that this method is also applicable to porcine somatic NT.
Booth, P J +3 more
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A Double Nuclear Transfer Technique for Cloning Pigs
2006The first round of double nuclear transfer (NT) procedure includes the following steps: transfer of somatic cell nuclei into enucleated recipient oocytes, fusion, activation, and culture of reconstructed oocytes. The next day, a second round of NT is performed by removing karyoplasts from 1-d-old NT embryos and transferring them into in vivo-derived ...
Irina A, Polejaeva +2 more
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Techniques for Nuclear Transfer to Mouse Embryonic Stem Cells
2006In this chapter, methods are described that permit the enucleation of mouse embryonic stem (ES) cells and the transfer of donor nuclei to these cells before or after enucleation has taken place. The small size and high nucleus-to-cytoplasm volume ratio of ES cells poses a challenge to their enucleation.
Danièle, Pralong, Paul J, Verma
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Studies of inheritance in amoebae by the technique of nuclear transfer
Proceedings of the Royal Society of London. Series B - Biological Sciences, 1958Abstract The methods of classical genetics depend essentially on the reassortment of genes which occurs as a result of meiotic and mating processes. These methods are of great elegance and great power. Upon their success our present theory of inheritance depends. But these methods also have certain weaknesses.
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Biochemical and Biophysical Research Communications, 2006
The low success rate of animal cloning by somatic cell nuclear transfer (SCNT) is believed to be associated with epigenetic errors including abnormal DNA hypermethylation. Recently, we elucidated by using round spermatids that, after nuclear transfer, treatment of zygotes with trichostatin A (TSA), an inhibitor of histone deacetylase, can remarkably ...
Satoshi, Kishigami +7 more
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The low success rate of animal cloning by somatic cell nuclear transfer (SCNT) is believed to be associated with epigenetic errors including abnormal DNA hypermethylation. Recently, we elucidated by using round spermatids that, after nuclear transfer, treatment of zygotes with trichostatin A (TSA), an inhibitor of histone deacetylase, can remarkably ...
Satoshi, Kishigami +7 more
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Cloning and Stem Cells, 2001
Contemporary nuclear transfer techniques often require the involvement of skilled personnel and extended periods of micromanipulation. Here, we present details of the development of a nuclear transfer technique for somatic cells that is both simpler and faster than traditional methods.
Booth, P J +4 more
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Contemporary nuclear transfer techniques often require the involvement of skilled personnel and extended periods of micromanipulation. Here, we present details of the development of a nuclear transfer technique for somatic cells that is both simpler and faster than traditional methods.
Booth, P J +4 more
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Theriogenology, 2003
This study was conducted to improve a porcine somatic cell nuclear transfer (SCNT) technique by optimizing donor cell and recipient oocyte preparations. Adult and fetal fibroblasts, and cumulus and oviduct cells were used as donor cells, and in vivo- and in vitro-matured oocytes were employed as recipient oocytes.
Gab-sang, Lee +9 more
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This study was conducted to improve a porcine somatic cell nuclear transfer (SCNT) technique by optimizing donor cell and recipient oocyte preparations. Adult and fetal fibroblasts, and cumulus and oviduct cells were used as donor cells, and in vivo- and in vitro-matured oocytes were employed as recipient oocytes.
Gab-sang, Lee +9 more
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Theriogenology, 2006
Routine activation of nuclear transfer (NT) eggs involves the application of a single intracellular calcium [Ca2+]i rise, stimulated by an electrical pulse, as opposed to [Ca2+]i oscillations, which is the natural mode of sperm-induced activation at fertilization in all mammalian species tested to date.
Teru, Jellerette +6 more
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Routine activation of nuclear transfer (NT) eggs involves the application of a single intracellular calcium [Ca2+]i rise, stimulated by an electrical pulse, as opposed to [Ca2+]i oscillations, which is the natural mode of sperm-induced activation at fertilization in all mammalian species tested to date.
Teru, Jellerette +6 more
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Volume 8B: Heat Transfer and Thermal Engineering, 2015
The experimental investigations were carried out of a pressurized water nuclear reactor (PWR) with enhanced surface using different concentration (0.5 and 2.0 vol%) of ZnO/DI-water based nanofluids as a coolant. The experimental setup consisted of a flow loop with a nuclear fuel rod section that was heated by electrical current.
Kang Liu +3 more
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The experimental investigations were carried out of a pressurized water nuclear reactor (PWR) with enhanced surface using different concentration (0.5 and 2.0 vol%) of ZnO/DI-water based nanofluids as a coolant. The experimental setup consisted of a flow loop with a nuclear fuel rod section that was heated by electrical current.
Kang Liu +3 more
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Animal Science Journal, 2010
ABSTRACTAnimal biotechnology has made new biological experiments possible and new discoveries are being made on an almost daily basis. Among these discoveries is a method for directly injecting a spermatozoa or somatic nucleus into an oocyte that has brought a revolution in the world of micromanipulators. Experiments that were unfeasible until now have
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ABSTRACTAnimal biotechnology has made new biological experiments possible and new discoveries are being made on an almost daily basis. Among these discoveries is a method for directly injecting a spermatozoa or somatic nucleus into an oocyte that has brought a revolution in the world of micromanipulators. Experiments that were unfeasible until now have
openaire +2 more sources