Rapid clonal identification of biallelic CRISPR/Cas9 knock-ins using SNEAK PEEC
Scientific Reports, 2023 One of the challenges faced by current CRISPR/Cas9 editing strategies is the difficulty in rapidly selecting clonal populations of biallelically edited cells.
Sameer Singh +3 more
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Medicina, 2022 Arboviruses have been reported over the years as constant threats to blood transfusion recipients, given the high occurrence of asymptomatic cases and the fact that the presence of viremia precedes the onset of symptoms, making it possible that infected ...
Leticia Martins Lamarão +10 more
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Serpentine and Vermiform Are Produced Autonomously to Fulfill Their Function in Drosophila Wings
Insects, 2023 Group I chitin deacetylases (CDAs), CDA1 and CDA2, play an essential role in cuticle formation and molting in the process of insect wing development. A recent report showed that trachea are able to take up a secreted CDA1 (serpentine, serp) produced in ...
Xubo Zhang +6 more
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Specificity Determination in Saccharomyces cerevisiae Killer Virus Systems
Microorganisms, 2021 Saccharomyces yeasts are widely distributed in the environment and microbiota of higher organisms. The killer phenotype of yeast, encoded by double-stranded RNA (dsRNA) virus systems, is a valuable trait for host survival. The mutual relationship between
Lina Aitmanaitė +4 more
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CRISPR-based nucleic acid diagnostics for pathogens [PDF]
, 2022 Pathogenic infections remain the primary threat for human health, especially the global COVID-19 pandemic. It is important to develop rapid, sensitive and multiplexed tools for detecting pathogens and their mutations, particularly the tailor-made ...
Yong, Zhang +5 more
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Algorithms for Nucleic Acid Sequence Design [PDF]
, 2010 Motivated by a growing field of research focused on programming function into biomolecules, we seek to decrease the cost of high-quality rational nucleic acid sequence design while increasing its versatility and availability.
Zadeh, Joseph N.
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Branching Ionizable Lipids Can Enhance the Stability, Fusogenicity, and Functional Delivery of mRNA
Small Science, 2023 Ionizable lipids with branched tails have been used in lipid nanoparticles (LNPs)‐based messenger RNA (mRNA) therapeutics like COVID‐19 vaccines. However, due to the limited commercial availability of branched ingredients, a systematic analysis of how ...
Kazuki Hashiba +9 more
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Non-canonical DNA structures: Diversity and disease association
Frontiers in Genetics, 2022 A complete understanding of DNA double-helical structure discovered by James Watson and Francis Crick in 1953, unveil the importance and significance of DNA.
Aparna Bansal +4 more
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RNA ligase ribozymes with a small catalytic core
Scientific Reports, 2023 Catalytic RNAs, or ribozymes, catalyze diverse chemical reactions that could have sustained primordial life in the hypothetical RNA world. Many natural ribozymes and laboratory evolved ribozymes exhibit efficient catalysis mediated by elaborate catalytic
Yoko Nomura, Yohei Yokobayashi
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Studies on the relation between heme and nucleic acid syntheses in erythroid cell. II. Nucleic acid synthesis in erythroblast of anemic rat treated with aminopterin and bromouracil [PDF]
, 1968 With the bone marrow of anemic rats, which had received the repeated injections of phenylhydrazine once a day for three to four days, the effects of aminopterin and bromouracil on the nucleic acid metabolism of erythroblasts were observed in vivo ...
Shigehisa, Morio
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