Labeling of Unique Sequences in Double-Stranded DNA at Sites of Vicinal Nicks Generated by Nicking Endonucleases [PDF]
, 2008 We describe a new approach for labeling of unique sequences within dsDNA under nondenaturing conditions. The method is based on the site-specific formation of vicinal nicks, which are created by nicking endonucleases (NEases) at specified DNA sites on ...
Frank-Kamenetskii, Maxim D., Kuhn, Heiko
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Microfluidic-Based Nucleic Acid Amplification Systems in Microbiology
Micromachines, 2019 Rapid, sensitive, and selective bacterial detection is a hot topic, because the progress in this research area has had a broad range of applications.
Lena Gorgannezhad +2 more
doaj +1 more source
Recent Improvements in CRISPR-Based Amplification-Free Pathogen Detection
Frontiers in Microbiology, 2021 Molecular diagnostic (MDx) methods directly detect target nucleic acid sequences and are therefore an important approach for precise diagnosis of pathogen infection. In comparison with traditional MDx techniques such as PCR, the recently developed CRISPR-
Jian Zhang +9 more
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Recent advances and perspectives of nucleic acid detection for coronavirus
Journal of Pharmaceutical Analysis, 2020 The recent pneumonia outbreak caused by a novel coronavirus (SARS-CoV-2) is posing a great threat to global public health. Therefore, rapid and accurate identification of pathogenic viruses plays a vital role in selecting appropriate treatments, saving ...
Minzhe Shen +6 more
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Prospects of Microfluidic Technology in Nucleic Acid Detection Approaches
Biosensors, 2023 Conventional diagnostic techniques are based on the utilization of analyte sampling, sensing and signaling on separate platforms for detection purposes, which must be integrated to a single step procedure in point of care (POC) testing devices.
Zilwa Mumtaz +6 more
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tmRNA - a novel high-copy-number RNA diagnostic target - its application for Staphylococcus aureus detection using real-time NASBA [PDF]
, 2009 A real-time nucleic acid sequence-based amplification assay, targeting tmRNA, was designed for the rapid identification of Staphylococcus aureus. The selectivity of the assay was confirmed against a panel of 76 Staphylococcus strains and species and 22 ...
Anonymous +9 more
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The Application of Hybridization Chain Reaction in the Detection of Foodborne Pathogens
Foods, 2023 Today, with the globalization of the food trade progressing, food safety continues to warrant widespread attention. Foodborne diseases caused by contaminated food, including foodborne pathogens, seriously threaten public health and the economy.
Jinbin Zhao +7 more
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An ultrasensitive photoelectrochemical nucleic acid biosensor [PDF]
, 2005 A simple and ultrasensitive procedure for non-labeling detection of nucleic acids is described in this study. It is based on the photoelectrochemical detection of target nucleic acids by forming a nucleic acid/photoreporter adduct layer on an ITO ...
Gao, Zhiqiang, Tansil, Natalia C.
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Bioaffinity detection of pathogens on surfaces [PDF]
, 2010 The demand for improved technologies capable of rapidly detecting pathogens with high sensitivity and selectivity in complex environments continues to be a significant challenge that helps drive the development of new analytical techniques. Surface-based
Faisal, S. N. +4 more
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Exploration of isothermal nucleic acid amplification techniques in the biomedical field
Gene and Genome EditingSukanya Kundu +2 more
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