Results 41 to 50 of about 186,936 (344)

Nucleic acid purification from plants, animals and microbes in under 30 seconds.

open access: yesPLoS Biology, 2017
Nucleic acid amplification is a powerful molecular biology tool, although its use outside the modern laboratory environment is limited due to the relatively cumbersome methods required to extract nucleic acids from biological samples.
Yiping Zou   +7 more
doaj   +1 more source

Rapid and Highly Sensitive Detection of Leishmania by Combining Recombinase Polymerase Amplification and Solution-Processed Oxide Thin-Film Transistor Technology

open access: yesBiosensors, 2023
Nucleic acid detection is widely used to identify infectious diseases and ensure food safety. However, conventional PCR-based techniques are time consuming.
Weidong Wu   +3 more
doaj   +1 more source

Performance and accuracy of SD Bioline malaria Ag P.f (05fk50) in relation to microscopy as the gold standard technique for malaria diagnosis [PDF]

open access: yesCellular, Molecular and Biomedical Reports, 2023
Successful malaria diagnosis is the mainstay of successful treatment, prevention and eradication of malaria infection. Apart from the gold standard technique (Microscopy), numerous diagnostic techniques perform a similar function to microscopy and in ...
Ismail Muhammad   +5 more
doaj   +1 more source

Monolithic centrifugal microfluidic platform for bacteria capture and concentration, lysis, nucleic-acid amplification, and real-time detection [PDF]

open access: yes, 2009
We report the design, fabrication, and characterization of a polymer centrifugal microfluidic system for the specific detection of bacterial pathogens.
Barry, Thomas   +5 more
core   +1 more source

Unexpected detection of DNA by nucleic acid sequence-based amplification technique

open access: yesMolecular and Cellular Probes, 2004
Nucleic acid sequence-based amplification (NASBA) is a technique that has been previously shown to selectively mediate the detection of RNA in microbial cells. In a series of tests, nucleic acids were extracted from Salmonella enterica serotype Typhimurium and Mycobacterium avium subsp. paratuberculosis, and subjected to four enzymatic treatments prior
David, Rodríguez-Làzaro   +4 more
openaire   +2 more sources

Development of a nucleic acid lateral flow immunoassay for simultaneous detection of Listeria spp. and Listeriamonocytogenes in food [PDF]

open access: yes, 2009
We present a new nucleic acid lateral flow immunoassay (NALFIA) for the assessment of listeria contamination. The detection procedure starts with enrichment of sample in Half Fraser broth (24 h). Following isolation of DNA, a duplex PCR is performed with
Amerongen, A., van   +3 more
core   +2 more sources

Application of nucleic acid signal amplification in biosensing and bioimaging

open access: yesBiomedical Technology
Nucleic acid amplification techniques are broadly employed in nucleic acid testing due to their efficient accumulation of nucleic acid sequences. With the development of biotechnology, they are further applied to proteins, living cells, extracellular ...
Junqi Zhao   +3 more
doaj   +1 more source

Advances in Microfluidics and Lab-on-a-Chip Technologies

open access: yes, 2017
Advances in molecular biology are enabling rapid and efficient analyses for effective intervention in domains such as biology research, infectious disease management, food safety, and biodefense.
Abate   +161 more
core   +1 more source

Tuberculosis diagnostics and biomarkers: needs, challenges, recent advances, and opportunities [PDF]

open access: yes, 2012
Tuberculosis is unique among the major infectious diseases in that it lacks accurate rapid point-of-care diagnostic tests. Failure to control the spread of tuberculosis is largely due to our inability to detect and treat all infectious cases of pulmonary
Alexandre Harari   +118 more
core   +2 more sources

Clinical application of high throughput molecular screening techniques for pharmacogenomics. [PDF]

open access: yes, 2011
Genetic analysis is one of the fastest-growing areas of clinical diagnostics. Fortunately, as our knowledge of clinically relevant genetic variants rapidly expands, so does our ability to detect these variants in patient samples.
Schrijver, Iris, Wiita, Arun P
core   +1 more source

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