Nucleic acid purification from plants, animals and microbes in under 30 seconds.
Nucleic acid amplification is a powerful molecular biology tool, although its use outside the modern laboratory environment is limited due to the relatively cumbersome methods required to extract nucleic acids from biological samples.
Yiping Zou +7 more
doaj +1 more source
Nucleic acid detection is widely used to identify infectious diseases and ensure food safety. However, conventional PCR-based techniques are time consuming.
Weidong Wu +3 more
doaj +1 more source
Performance and accuracy of SD Bioline malaria Ag P.f (05fk50) in relation to microscopy as the gold standard technique for malaria diagnosis [PDF]
Successful malaria diagnosis is the mainstay of successful treatment, prevention and eradication of malaria infection. Apart from the gold standard technique (Microscopy), numerous diagnostic techniques perform a similar function to microscopy and in ...
Ismail Muhammad +5 more
doaj +1 more source
Monolithic centrifugal microfluidic platform for bacteria capture and concentration, lysis, nucleic-acid amplification, and real-time detection [PDF]
We report the design, fabrication, and characterization of a polymer centrifugal microfluidic system for the specific detection of bacterial pathogens.
Barry, Thomas +5 more
core +1 more source
Unexpected detection of DNA by nucleic acid sequence-based amplification technique
Nucleic acid sequence-based amplification (NASBA) is a technique that has been previously shown to selectively mediate the detection of RNA in microbial cells. In a series of tests, nucleic acids were extracted from Salmonella enterica serotype Typhimurium and Mycobacterium avium subsp. paratuberculosis, and subjected to four enzymatic treatments prior
David, Rodríguez-Làzaro +4 more
openaire +2 more sources
Development of a nucleic acid lateral flow immunoassay for simultaneous detection of Listeria spp. and Listeriamonocytogenes in food [PDF]
We present a new nucleic acid lateral flow immunoassay (NALFIA) for the assessment of listeria contamination. The detection procedure starts with enrichment of sample in Half Fraser broth (24 h). Following isolation of DNA, a duplex PCR is performed with
Amerongen, A., van +3 more
core +2 more sources
Application of nucleic acid signal amplification in biosensing and bioimaging
Nucleic acid amplification techniques are broadly employed in nucleic acid testing due to their efficient accumulation of nucleic acid sequences. With the development of biotechnology, they are further applied to proteins, living cells, extracellular ...
Junqi Zhao +3 more
doaj +1 more source
Advances in Microfluidics and Lab-on-a-Chip Technologies
Advances in molecular biology are enabling rapid and efficient analyses for effective intervention in domains such as biology research, infectious disease management, food safety, and biodefense.
Abate +161 more
core +1 more source
Tuberculosis diagnostics and biomarkers: needs, challenges, recent advances, and opportunities [PDF]
Tuberculosis is unique among the major infectious diseases in that it lacks accurate rapid point-of-care diagnostic tests. Failure to control the spread of tuberculosis is largely due to our inability to detect and treat all infectious cases of pulmonary
Alexandre Harari +118 more
core +2 more sources
Clinical application of high throughput molecular screening techniques for pharmacogenomics. [PDF]
Genetic analysis is one of the fastest-growing areas of clinical diagnostics. Fortunately, as our knowledge of clinically relevant genetic variants rapidly expands, so does our ability to detect these variants in patient samples.
Schrijver, Iris, Wiita, Arun P
core +1 more source

