Results 11 to 20 of about 205,585 (348)

Effects of 2'-O-methyl nucleotide substitution on EcoRI endonuclease cleavage activities. [PDF]

PLoS ONE, 2013
To investigate the effect of sugar pucker conformation on DNA-protein interactions, we used 2'-O-methyl nucleotide (2'-OMeN) to modify the EcoRI recognition sequence -TGAATTCT-, and monitored the enzymatic cleavage process using FRET method.
Guojie Zhao, Bin Zhao, Zhaoxue Tong, Runqing Mu, Yifu Guan   +4 more
doaj   +1 more source

Optimizing scoring function of protein-nucleic acid interactions with both affinity and specificity. [PDF]

PLoS ONE, 2013
Protein-nucleic acid (protein-DNA and protein-RNA) recognition is fundamental to the regulation of gene expression. Determination of the structures of the protein-nucleic acid recognition and insight into their interactions at molecular level are vital ...
Zhiqiang Yan, Jin Wang
doaj   +1 more source

Structural basis of R-loop recognition by the S9.6 monoclonal antibody

Nature Communications, 2022
The S9.6 monoclonal antibody is widely used to map R-loops genome wide. Here, Bou-Nader et al., define the nucleic acid-binding specificity of S9.6 and report its crystal structures free and bound to a hybrid, which reveal the asymmetric recognition of ...
Charles Bou-Nader, Ankur Bothra, David N. Garboczi, Stephen H. Leppla, Jinwei Zhang   +4 more
doaj   +1 more source

Metal-Binding Antimicrobial Peptide SIF4 Kills Escherichia coli by Targeting Cytoplasmic Biomacromolecules without Cytoplasmic Membrane Damage: A Mechanistic Study [PDF]

Shipin Kexue, 2023
To explore how metal-binding antimicrobial peptide SIF4 kills foodborne Escherichia coli by targeting nucleic acid and protein in the cytoplasmic membrane without cytoplasmic membrane damage, the effect of SIF4 on intracellular nucleic acid biosynthesis ...
LI Yuzhen, XIAO Huaiqiu, LIU Miao, WANG Lin, ZENG Mengqi, ZHAO Mouming
doaj   +1 more source

Conformational analysis of nucleic acids revisited: Curves+ [PDF]

Nucleic Acids Research, 2009
We describe Curves+, a new nucleic acid conformational analysis program which is applicable to a wide range of nucleic acid structures, including those with up to four strands and with either canonical or modified bases and backbones. The program is algorithmically simpler and computationally much faster than the earlier Curves approach, although it ...
Lavery, R., Moakher, M., Maddocks, J. H., Petkeviciute, D., Zakrzewska, K.   +4 more
openaire   +3 more sources

Development of purification process for dual‐function recombinant human heavy‐chain ferritin by the investigation of genetic modification impact on conformation

Engineering in Life Sciences, 2021
Ferritin is a promising drug delivery platform and has been functionalized through genetic modifications. This work has designed and expressed a dual‐functional engineered human heavy‐chain ferritin (HFn) with the inserted functional peptide PAS and RGDK
Shuang Yin, Bingyang Zhang, Jianying Lin, Yongdong Liu, Zhiguo Su, Jingxiu Bi   +5 more
doaj   +1 more source

Conformational analysis of Infectious bursal disease virus (IBDV) derived cell penetrating peptide (CPP) analogs

Veterinary World, 2013
Aim: This study was designed to develop peptide analogs of Infectious Bursal Disease (IBD) virus VP5 protein segment having cell penetrating ability to improve their interaction with cargo molecule (Nucleic acid) without affecting the backbone ...
Vinay G. Joshi, Arvind Kumar Singh, Kantaraja Chindera, Manish V. Bais, Ashok Kumar Tiwari, Satish Kumar   +5 more
doaj   +1 more source

Nucleic acid-based biosensors: analytical devices for prevention, diagnosis and treatment of diseases

Vitae, 2021
BACKGROUND : Biosensing techniques have been the subject of exponentially increasing interest due to their performance advantages such as high selectivity and sensitivity, easy operation, low cost, short analysis time, simple sample preparation, and real-
Laura Carvajal Barbosa, Diego Insuasty Cepeda, Andrés Felipe León Torres, Maria Mercedes Arias Cortes, Zuly Jenny Rivera Monroy, Javier Eduardo Garcia Castaneda   +5 more
doaj   +1 more source

DNA Renaturation at the Water-Phenol Interface [PDF]

, 2003
We study DNA adsorption and renaturation in a water-phenol two-phase system, with or without shaking. In very dilute solutions, single-stranded DNA is adsorbed at the interface in a salt-dependent manner.
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core   +4 more sources

Spectrophotometric and nucleic acid-binding properties of halloysite clay nanotubes and kaolinite

Heliyon, 2023
Halloysite particles (HNTs) are naturally occurring aluminosilicate nanotubes of low toxicity that have shown great promise for drug and biomolecule delivery into human and animal cells.
Shubha R.L. Malla, Archana Gujjari, Carlos E. Corona, Gary W. Beall, L. Kevin Lewis   +4 more
doaj   +1 more source