Results 21 to 30 of about 95,624 (263)

The conformation of single‐stranded nucleic acids [PDF]

European Journal of Biochemistry, 1990
Conformational analyses using the single‐strand‐specific nuclease from mung bean and restriction endonucleases have been performed on a series of DNA fragments related to the sequence of the yeast initiator tRNAMet. Mung bean nuclease cleaves DNA fragments exclusively in some, but not all, single‐stranded regions as predicted by RNA secondary rules ...
J, Paquette, K, Nicoghosian, G R, Qi, N, Beauchemin, R, Cedergren   +4 more
openaire   +2 more sources

Insights on the DNA Stability in Aqueous Solutions of Ionic Liquids

Frontiers in Bioengineering and Biotechnology, 2020
Deoxyribonucleic acid (DNA) carries the genetic information essential for the growth and functioning of living organisms, playing a significant role in life sciences research.
Teresa B. V. Dinis, Fani Sousa, Mara G. Freire   +2 more
doaj   +1 more source

Microgels and apparatus for PAGE of nucleic acids in one or two dimensions

BioTechniques, 2018
We describe a system for horizontal 1D or 2D PAGE comprising an apparatus and microgels. There is no buffer outside the gel, making handling and sample loading easy.
Hans G Thormar, Gudmundur H Gunnarsson, Bjarki Gudmundsson, Kristjan Leosson, Peter Estibeiro, Jon J Jonsson   +5 more
doaj   +1 more source

T7 RNA polymerase functions in vitro without clustering. [PDF]

PLoS ONE, 2012
Many nucleic acid polymerases function in clusters known as factories. We investigate whether the RNA polymerase (RNAP) of phage T7 also clusters when active.
Kieran Finan, Joseph P Torella, Achillefs N Kapanidis, Peter R Cook   +3 more
doaj   +1 more source

IFITs: Emerging Roles As Key Anti-Viral Proteins

Frontiers in Immunology, 2014
Interferon-induced protein with tetratricopeptide repeats (IFITs) are a family of proteins which are strongly induced downstream of type I interferon (IFN) signaling.
Gregory I Vladimer, Maria W Górna, Giulio eSuperti-Furga   +2 more
doaj   +1 more source

First High-Resolution Crystal Structures of DNA:2′-O-Methyl-RNA Heteroduplexes

Crystals, 2022
Heteroduplexes composed of all-DNA and all-2′-OMe RNA strands do not occur in nature, but they have found application in the development of molecular beacons and could also be used as aptamers or elements of nucleic acid-based nanostructures that will ...
Rafał Dolot, Anna Maciaszek, Barbara Mikołajczyk, Barbara Nawrot   +3 more
doaj   +1 more source

Use of Nucleic Acid Analogs for the Study of Nucleic Acid Interactions

Journal of Nucleic Acids, 2011
Unnatural nucleosides have been explored to expand the properties and the applications of oligonucleotides. This paper briefly summarizes nucleic acid analogs in which the base is modified or replaced by an unnatural stacking group for the study of ...
Shu-ichi Nakano, Masayuki Fujii, Naoki Sugimoto   +2 more
doaj   +1 more source

Lone Pair…π Contacts and Structure Signatures of r(UNCG) Tetraloops, Z-Turns, and Z-Steps: A WebFR3D Survey

Molecules, 2022
Z-DNA and Z-RNA have long appeared as oddities to nucleic acid scientists. However, their Z-step constituents are recurrently observed in all types of nucleic acid systems including ribosomes.
Craig L. Zirbel, Pascal Auffinger
doaj   +1 more source

Conformational Dynamics of Biopolymers in the Course of Their Interaction: Multifaceted Approaches to the Analysis by the Stopped-Flow Technique with Fluorescence Detection

Photonics, 2023
This review deals with modern approaches to systematic research on molecular-kinetic mechanisms of damage recognition and removal by pro- and eukaryotic enzymes of DNA base excision repair.
Nikita A. Kuznetsov
doaj   +1 more source

A Chimeric DNA/RNA Antiparallel Quadruplex with Improved Stability

ChemistryOpen, 2022
Nucleic acid quadruplexes are proposed to play a role in the regulation of gene expression, are often present in aptamers selected for specific binding functions and have potential applications in medicine and biotechnology.
Elaina P. Boyle, Dr. Levan Lomidze, Prof. Dr. Karin Musier‐Forsyth, Prof. Dr. Besik Kankia   +3 more
doaj   +1 more source