Advanced Science, EarlyView.CRISPR/Cas9, while transformative, faces challenges in specificity, precision, delivery, accessibility, flexibility, and safety. This review addresses these limitations by highlighting strategies to reduce off‐target effects, exploring HDR‐based and alternative editing approaches, and evaluating advanced delivery mechanisms.
Muna Alariqi +8 more
wiley +1 more source
Structural flexibility in the ordered domain of the dengue virus strain 2 capsid protein is critical for chaperoning viral RNA replication. [PDF]
Cell Mol Life SciSharma KK +5 more
europepmc +1 more source
An Engineered PfAgo with Wide Catalytic Temperature Range and Substrate Spectrum
Advanced Science, EarlyView.This work presents a strategy for engineering thermophilic Ago proteins to obtain wide catalytic temperature range and broad substrate spectrum. This strategy is applied to engineer PfAgo and TtdAgo, and the biochemical properties of the engineered PfAgo are systematically characterized. The study further elucidates the structural differences involved.
Longyu Wang +11 more
wiley +1 more source
Supersecondary Structure Code for RNA: Trace of Conformational Change on the Mycoplasma pneumoniae Ribosome and the R-Loop Formation of Cas9. [PDF]
ACS OmegaIzumi H, Nafie LA, Dukor RK.
europepmc +1 more source
From Code to Life: The AI‐Driven Revolution in Genome Editing
Advanced Science, EarlyView.This review explores AI‐driven advancements in genome editing, focusing on CRISPR optimization, enhanced targeting precision, reduced off‐target effects, and novel tool design. It addresses ethical challenges, data biases, and highlights future prospects in precision medicine, agriculture, and synthetic biology.
Zhidong Li +10 more
wiley +1 more source
Unique conformational dynamics and protein recognition of A-to-I hyper-edited dsRNA. [PDF]
Nucleic Acids ResMüller-Hermes C +6 more
europepmc +1 more source
Single-stranded nucleic acid elasticity arises from internal electrostatic tension
Proceedings of the National Academy of Sciences of the United States of America, 2017 David R. Jacobson +4 more
semanticscholar +1 more source
Advanced Science, EarlyView.The Semaphorin 3E (Sema3E)‐Plexin‐D1 pathway mediated by C/EBPα and C/EBPβ downstream of the luteinizing hormone (LH) surge plays important roles in the mouse preovulatory ovary. Timely activation and suppression of this pathway during the preovulatory stage are crucial for ovulation, corpus luteum formation, and proper angiogenesis.
Hanxue Zhang +11 more
wiley +1 more source
A rational engineering strategy for structural dynamics modulation enables target specificity enhancement of the Cas9 nuclease. [PDF]
Nucleic Acids ResSung K +6 more
europepmc +1 more source
Conformational analysis of nucleic acids Extended Hückel study on the D‐ribose (C3, ‐exo)‐phosphate unit [PDF]
, 1972 D. Vasilescu +2 more
openalex +1 more source