Results 71 to 80 of about 438,619 (385)
npj Biofilms and Microbiomes, 2021 Extracellular DNA, or eDNA, is recognised as a critical biofilm component; however, it is not understood how it forms networked matrix structures. Here, we isolate eDNA from static-culture Pseudomonas aeruginosa biofilms using ionic liquids to preserve ...
Thomas Seviour +14 more
doaj +1 more source
TFE and Spt4/5 open and close the RNA polymerase clamp during the transcription cycle [PDF]
, 2016 Transcription is an intrinsically dynamic process and requires the coordinated interplay of RNA polymerases (RNAPs) with nucleic acids and transcription factors.
Gietl, Andreas +5 more
core +1 more source
Purification tags markedly affect self‐aggregation of CPEB3
FEBS Letters, EarlyView.Although recombinant proteins are used to study protein aggregation in vitro, uncleaved tags can interfere with accurate interpretation. Our findings demonstrate that His₆‐GFP and His₁₂ tags significantly affect liquid droplet and amyloid fibril formation in the intrinsically disordered region (IDR) of mouse cytoplasmic polyadenylation element‐binding ...
Harunobu Saito +6 more
wiley +1 more source
Electrostatics of Nucleic Acid Folding under Conformational Constraint [PDF]
Journal of the American Chemical Society, 2012 RNA folding is enabled by interactions between the nucleic acid and its ion atmosphere, the mobile sheath of aqueous ions that surrounds and stabilizes it. Understanding the ion atmosphere requires the interplay of experiment and theory. However, even an apparently simple experiment to probe the ion atmosphere, measuring the dependence of DNA duplex ...
Vincent B. Chu +5 more
openaire +3 more sources
Structural alphabets for conformational analysis of nucleic acids available at dnatco.datmos.org [PDF]
Acta Crystallographica Section D Structural Biology, 2020 A detailed description of the dnatco.datmos.org web server implementing the universal structural alphabet of nucleic acids is presented. It is capable of processing any mmCIF- or PDB-formatted files containing DNA or RNA molecules; these can either be uploaded by the user or supplied as the wwPDB or PDB-REDO structural database access code.
Jiří Černý +5 more
openaire +3 more sources
Structural properties of the linkers connecting the n- and c- terminal domains in the mocr bacterial transcriptional regulators [PDF]
, 2016 Peptide inter-domain linkers are peptide segments covalently linking two adjacent domains within a protein. Linkers play a variety of structural and functional roles in naturally occurring proteins.
ANGELACCIO, Sebastiana +5 more
core +3 more sources
FEBS Letters, EarlyView.In the adult T‐cell leukemia/lymphoma (ATL) cell line ED, the human T‐cell leukemia virus type 1 (HTLV‐1) provirus was integrated into the intron of the ift81 gene in the antisense orientation. Despite this integration, both the intact ift81 and the viral oncogene hbz were simultaneously expressed, likely due to the functional insufficiency of viral ...
Mayuko Yagi +5 more
wiley +1 more source
Nanomaterials, 2019 Nucleic acid-based technologies are an emerging research focus area for pharmacological and biological studies because they are biocompatible and can be designed to produce a variety of scaffolds at the nanometer scale.
Ryan C. Oliver +4 more
doaj +1 more source
Frontiers in Cell and Developmental Biology, 2021 Herein we report that the 18-base telomeric oligodeoxynucleotides (ODNs) designed from the Lactobacillus rhamnosus GG genome promote differentiation of skeletal muscle myoblasts which are myogenic precursor cells. We termed these myogenetic ODNs (myoDNs).
Sayaka Shinji +12 more
doaj +1 more source
Journal of the American Chemical Society, 2017 Here we report the investigation of glycol nucleic acid (GNA), an acyclic nucleic acid analogue, as a modification of siRNA duplexes. We evaluated the impact of (S)- or (R)-GNA nucleotide incorporation on RNA duplex structure by determining three ...
M. Schlegel +13 more
semanticscholar +1 more source