Results 171 to 180 of about 60,672 (308)

Digital Melting Curve Analysis for Multiplex Quantification of Nucleic Acids on Droplet Digital PCR. [PDF]

Biosensors (Basel)
Dai X, Cao M, Wang Z.
europepmc   +1 more source

Transcriptional response in larvae of the generalist fruit fly Anastrepha obliqua feeding on three different tropical host plants

Entomologia Experimentalis et Applicata, EarlyView.
We investigated the molecular basis of host switching by the phytophagous West Indian fruit fly (A. obliqua). Third‐instar larvae showed differential gene expression according to host plants: red mombin, mango, and carambola. The main gene categories differentially expressed were digestion, detoxification, and gene regulators.
Sandra M. Velasco‐Cuervo, César A. Lemos‐Lucumi, Maria R. Manzano, Stephen E. Strelkov, Nelson Toro‐Perea, Leonardo Galindo‐González   +5 more
wiley   +1 more source

Investigation of four diagnostic methods to identify infectious ulcerative keratitis in horses in Colorado, USA

Equine Veterinary Education, EarlyView.
Summary Background Ulcerative keratitis in equine patients can result in keratomalacia and perforation. Prompt and accurate diagnostics are necessary for appropriate therapy and a favourable outcome. Objective To determine the diagnostic utility (specificity and speed) of four diagnostic methods for infectious ulcerative keratitis in the horse.
K. E. V. Jones, M. R. Lappin, J. B. Daniels, J. Sharp, J. Hawley, M. d. L. Henriksen   +5 more
wiley   +1 more source

Protocol to Mine Unknown Flanking DNA Using PER-PCR for Genome Walking. [PDF]

Bio Protoc
Yu Z, Wang D, Lin Z, Li H.
europepmc   +1 more source

A loop‐mediated isothermal amplification assay to detect insect‐associated Serratia spp. in the critically endangered insect Dryococelus australis

Insect Conservation and Diversity, EarlyView.
Inclusion of a novel sample cleanup step prior to extraction of bacterial DNA from insect frass enhanced rates of bacterial cell recovery and removed insect and plant cells. Development of a robust LAMP assay allowed detection of insect‐associated members of the Serratia marcescens complex in frass from live insects. Results from the LAMP assay applied
Nicholas P. Doidge, Joanne L. Allen, Rhys Bushell, Michael Lynch, Glenn F. Browning, Marc S. Marenda   +5 more
wiley   +1 more source

Mass photometry as a fast, facile characterization tool for direct measurement of mRNA length. [PDF]

Biol Methods Protoc
Schmudlach A, Spear S, Hua Y, Fertier-Prizzon S, Kochling J.   +4 more
europepmc   +1 more source

Molecular Identification of Agrilus anxius (Coleoptera: Buprestidae) Using a qPCR Assay With Locked Nucleic Acid (LNA) Probe

Journal of Applied Entomology, EarlyView.
ABSTRACT In the present study, a biomolecular diagnostic assay based on qPCR‐LNA (Locked Nucleic Acid) probes was developed for the identification of Agrilus anxius (Coleoptera: Buprestidae) from adult insects. The performance of the new protocol was also evaluated for indirect diagnosis of the insect's presence on artificially contaminated frass.
Domenico Rizzo, Francesco Pecori, Michela Moriconi, Claudia Gabriela Zubieta, Bruno Palmigiano, Linda Bartolini, Alice Downes, Chiara Ranaldi, Viola Papini, Nicola Luchi, Alberto Santini   +10 more
wiley   +1 more source

Point-of-Care Diagnostics Using Self-heating Elements from Smart Food Packaging: Moving Towards Instrument-Free Nucleic Acid-Based Detection. [PDF]

Mol Diagn Ther
Hamidizadeh M, Martins RF, Bier FF.
europepmc   +1 more source

Protocol to Identify Unknown Flanking DNA Using Partially Overlapping Primer-based PCR for Genome Walking. [PDF]

Bio Protoc
Jia M, Ding D, Liu X, Li H.
europepmc   +1 more source

Experimental Challenge of Chinook Salmon (Oncorhynchus tshawytscha) With Tenacibaculum maritimum and Tenacibaculum dicentrarchi Fulfils Koch's Postulates

Journal of Fish Diseases, EarlyView.
ABSTRACT The bacterial skin disease tenacibaculosis, caused by Tenacibaculum species, affects numerous economically important marine fish, including salmonids. This study reports the ability of three Tenacibaculum maritimum strains, belonging to different molecular O‐AGC types, and a single Tenacibaculum dicentrarchi strain to induce tenacibaculosis in
Karthiga Kumanan, Jeremy Carson, Ryan B. J. Hunter, Anne Rolton, Ulla von Ammon, Chaya Bandaranayake, Connie Angelucci, Richard N. Morrison, Seumas P. Walker, Jane E. Symonds, Kate S. Hutson   +10 more
wiley   +1 more source