ABSTRACT Environmental RNA (eRNA) metabarcoding has rapidly emerged as a powerful tool for assessing contemporary biodiversity patterns across diverse ecosystems. However, the potential for false positive detections caused by co‐extracted environmental DNA (eDNA) remains unquantified.
Fuwen Wang +4 more
wiley +1 more source
Ligase-Catalyzed Transcription and Reverse Transcription of XNA by T3 DNA Ligase. [PDF]
Khamissi N, Rustico L, Hili R.
europepmc +1 more source
Plant‐based fermentation matrices significantly influence microbial diversity and species‐specific proteomic adaptations. The integration of MALDI‐TOF MS and 16S rRNA sequencing with physicochemical analyses allowed for a better understanding of the impact of specific fermentation products on differences in microbial diversity and spectral protein ...
Agnieszka Ludwiczak +8 more
wiley +1 more source
ABSTRACT Introduction Black flies (Simuliidae) are globally distributed blood‐feeding arthropods and vectors of viral, bacterial, and parasitic pathogens to many animal species, including humans. We investigated the occurrence of selected vector‐borne pathogens in black flies in South Moravia, Czech Republic, and evaluated their possible role in the ...
Silvie Šikutová +10 more
wiley +1 more source
Missense mutation of <i>BMP1</i> may cause feline osteogenesis imperfecta without bone deformity. [PDF]
Takanosu M, Aoki H, Toshima A, Kagawa Y.
europepmc +1 more source
Abstract BACKGROUND Rising atmospheric carbon dioxide (CO2) is a key driver of climate change, making it essential to understand its effects on crop growth and metabolism. This study examines maize C01 (inbred) and B73 (mutant), under elevated CO2 (600, 1200 and 1800 ppm) at three growth stages [40, 70 and 90 days after sowing (DAS)].
Pirzada Khan +5 more
wiley +1 more source
The impact of heat inactivation on RT-per detection of severe acute respiratory syndrome corona virus 2 (SARS-CoV-2): an experience from the University Clinical Centre of Vojvodina, Serbia. [PDF]
Maletić JS +5 more
europepmc +1 more source
Versatile and Selective Biomolecule Pulldown with Combinatorial DNA‐Crosslinked Polymers
We present a programmable smart polymer system for universal, sequence‐selective bioseparation under native conditions. Polymer phase separation is achieved through combinatorial DNA crosslinking, enabling the capture of DNA, RNA, or proteins in a homogeneous solution with high pulldown efficiency and near‐zero background binding.
Sarah K. Speed +3 more
wiley +1 more source
Development of a TaqMan-Based Duplex Real-Time Quantitative RT-PCR for Detection and Differentiation of Muscovy Duck Reovirus and Novel Duck Reovirus. [PDF]
Liu L, Fu J, Lin M, Wang A, Wu S, Liu C.
europepmc +1 more source
Compartmentalization and Aggregation of Biomolecular Condensates in Crowded Hydrogels for Enhanced Nucleic Acid Diagnosis. [PDF]
A cell‐mimetic hydrogel compartment is proposed to enhance the efficiency of enzymatic reactions. The naturally porous structure of a crowded hydrogel can simulate the intracellular microenvironment, promoting the aggregation of biomolecules and activity of DNA polymerase through excluded volume effects and compartmentalization.
Xiao F, Yang T, Fang M, Lin X.
europepmc +2 more sources

