Results 181 to 190 of about 323,426 (336)

An All‐in‐One Metabarcoding Approach to Mosquito and Arbovirus Xenosurveillance

Molecular Ecology Resources, EarlyView.
ABSTRACT Next‐generation sequencing (NGS) has the potential to transform mosquito‐borne disease surveillance but remains under‐utilised. This study introduces a comprehensive multi‐loci metabarcoding‐based MX (molecular xenomonitoring) approach to mosquito and arbovirus surveillance, enabling parallel identification of mosquito vectors, circulating ...
Brian J. Johnson, Melissa C. Graham, Elina Panahi, Carla Julia S. P. Vieira, Nisa Suraj Nath, Paul Mason, Jamie Gleadhill, Darran Thomas, Michael B. Onn, Martin A. Shivas, Damien Shearman, Jonathan M. Darbro, Gregor J. Devine   +12 more
wiley   +1 more source

Evaluation of nucleic acid extraction methods for recovery of Cyclospora cayetanensis, Salmonella enterica, and murine norovirus from water and sludge. [PDF]

J Microbiol Methods
Kahler A, Hofstetter J, Rodrigues C, Mattioli M.   +3 more
europepmc   +1 more source

Comparative Decay Dynamics and Detectability of eDNA and eRNA in Connected and Isolated Freshwater Mesocosms Using Digital PCR

Molecular Ecology Resources, EarlyView.
ABSTRACT Efficient use of environmental nucleic acids (eNAs) in freshwater biodiversity monitoring requires understanding their degradation and detectability in interconnected ecosystems. We employed a novel field‐scale assay to compare environmental DNA (eDNA) and environmental RNA (eRNA) decay rates and detectability across four genetic markers (16S,
Wendy B. Morgado‐Gamero, Orianne Tournayre, Melania E. Cristescu   +2 more
wiley   +1 more source

Mie Scatter and Interfacial Tension Based Real‐Time Quantification of Colloidal Emulsion Nucleic Acid Amplification

Advanced Biosystems, 2017
Ariana M. Nicolini, Tyler D. Toth, Samuel Y. Kim, M. Mandel, D. Galbraith, Jeong‐Yeol Yoon   +5 more
semanticscholar   +1 more source

Refining Zooplankton Diet Composition Studies Over Short and Long Time Scales by Combining 18S Metabarcoding With Fatty Acid Analyses

Molecular Ecology Resources, EarlyView.
ABSTRACT Understanding diet composition is essential for unravelling trophic interactions in aquatic ecosystems. DNA metabarcoding, utilising various variable regions of the 18S rRNA gene, is increasingly employed to investigate zooplankton diet composition.
Nora‐Charlotte Pauli, Katja Metfies, Stefan Neuhaus, Martin Graeve, Alison C. Cleary, Morten H. Iversen, Bettina Meyer   +6 more
wiley   +1 more source

Integrative Multiparametric Analysis of Circulating Cell‐Free Nucleic Acids of Plasma in Healthy Individuals During Aging

Aging Cell, EarlyView.
We performed the first integrative multiparametric analysis of plasma circulating cell‐free nucleic acids, including nuclear and mitochondrial DNA, as well as ribosomal, messenger, and micro‐RNAs, in a cohort of 139 healthy individuals. We focused on quantity, integrity, and DNA methylation using assays targeting highly repetitive DNA and highly ...
Nicolas P. Tessier, Lise M. Hardy, Florence Mauger, Antoine Daunay, Christian Daviaud, Ilef Hchaichi, Caroline Horgues, Mourad Sahbatou, Hélène Le Buanec, Jean‐François Deleuze, Alexandre How‐Kit   +10 more
wiley   +1 more source

A rapid LASV detection method based on CRISPR-Cas13a and recombinase aided amplification with special lateral-flow test strips. [PDF]

Sci Rep
Wei T, Yan Y, Niu M, Dong X, Li H, Sun Y, Fa Y.   +6 more
europepmc   +1 more source

In Vitro Assessment of a Novel Piranha‐Passivated Dental Implant Surface Against Oral Biofilm Formation

Clinical Oral Implants Research, EarlyView.
ABSTRACT Background and Objectives Peri‐implantitis, a significant complication resulting from bacterial colonization on dental implants, presents a challenge in oral healthcare. Developing surfaces that inhibit bacterial adhesion while promoting tissue integration is crucial for improving implant outcomes.
Paula Nuevo, Leire Virto, Honorato Ribeiro‐Vidal, Javier Gil, Mariano Sanz   +4 more
wiley   +1 more source

Evaluation of Commercially Available Kits for Parallel DNA and microRNA Isolation Suitable for Epigenetic Analyses from Cell-Free Saliva and Salivary Extracellular Vesicles. [PDF]

Int J Mol Sci
Yousaf I, Kegler U, Hofner M, Noehammer C.   +3 more
europepmc   +1 more source

Investigation of four diagnostic methods to identify infectious ulcerative keratitis in horses in Colorado, USA

Equine Veterinary Education, EarlyView.
Summary Background Ulcerative keratitis in equine patients can result in keratomalacia and perforation. Prompt and accurate diagnostics are necessary for appropriate therapy and a favourable outcome. Objective To determine the diagnostic utility (specificity and speed) of four diagnostic methods for infectious ulcerative keratitis in the horse.
K. E. V. Jones, M. R. Lappin, J. B. Daniels, J. Sharp, J. Hawley, M. d. L. Henriksen   +5 more
wiley   +1 more source