Results 231 to 240 of about 60,957 (296)

Methods for thermal denaturation studies of nucleic acids in complex with fluorogenic dyes

, 2019
Thermal denaturation is a common technique in the biophysical study of nucleic acids. These experiments are typically performed by monitoring the increase in absorbance (hyperchromism) of a sample at 260nm with temperature (Mergny & Lacroix, 2003; Puglisi & Tinoco, 1989).
Lauren M. Aufdembrink, Tanner G. Hoog, Matthew R. Pawlak, Benjamin F. Bachan, Joseph M. Heili, Aaron E. Engelhart   +5 more
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Spatiotemporal features of nucleic acid hydration and their changes during denaturation revealed by THz spectroscopy

2015 40th International Conference on Infrared, Millimeter, and Terahertz waves (IRMMW-THz), 2015
Dynamics and structure of water network around nucleic acid have been subjects of intensive studies due to their contributions to biological process. Here, we identified spatiotemporal features of DNA hydration through examining fully-hydrated DNA solutions by THz spectroscopy. The results suggest the presence of weakly bound water beyond tight-binding
Heyjin Son, Jin Young Jeong, Sunmyoung Kim, Jaehun Park, Jeongmin Jang, Kihoon Eom, Inkyung Park, Gun‐Sik Park   +7 more
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The denaturation of desoxypentose nucleic acid

Biochimica et Biophysica Acta, 1955
Paul Doty, Stuart A. Rice
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The Alkaline Denaturation of Deoxyribose Nucleic Acid

Journal of the American Chemical Society, 1958
Paul Ehrlich, Paul Doty
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The reversible denaturation of DNA and its use in studies of nucleic acid homologies and the biological relatedness of microorganisms

Journal de Chimie Physique, 1961
Les chaines complementaires de la molecule d’ADN, qui sont liees l’une a l’autre par des liaisons hydrogene au niveau des paires de bases adenine-thymine et guanine-cytosine, peuvent etre separees par l’action de la chaleur, d’un acide, d’une base ou du formamide.
Julius Marmur, Carl L. Schildkraut, Paul Doty   +2 more
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Denaturation of Nucleic Acids Induced by Intercalating Agents. Biochemical and Biophysical Properties of Acridine Orange-DNA Complexes

Journal of Biomolecular Structure and Dynamics, 1984
At high binding densities acridine orange (AO) forms complexes with ds DNA which are insoluble in aqueous media. These complexes are characterized by high red- and minimal green-luminescence, 1:1 (dye/P) stoichiometry and resemble complexes of AO with ss nucleic acids.
Jan Kapuściński, Zbigniew Darżynkiewicz   +1 more
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Studies on the Structure of Nucleic Acids. X. On the Mechanism of Denaturation¹

Journal of the American Chemical Society, 1956
Liebe F. Cavalieri, Morton Rosoff, Barbara Hatch Rosenberg   +2 more
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Pyridine: a Denaturant or Stabilizer of Spherical Nucleic Acids?

Analytical Chemistry, 2017
The spotlighted dual functions of pyridine as a denaturant and as a stabilizer for duplex DNA are thoroughly investigated using spherical nucleic acids (SNAs). At neutral pH, pyridine destabilizes the duplex interconnects of assembled SNAs, resulting in a gradual decrease in their melting temperature (Tm) as a function of the pyridine concentration ...
Yoon Hyuck Kim, Ju-Hwan Oh, Abigail K. R. Lytton-Jean, Jae-Seung Lee   +3 more
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Microfluidic chip of fast DNA hybridization using denaturing and motion of nucleic acids [PDF]

ELECTROPHORESIS, 2008
AbstractThis study presents the effect of fluidic temperatures and velocities on improving DNA hybridization. The efficiency of hybridization could be improved by introducing elevated temperature in the hot region and velocity in the cold region. Compared with the conventional methods, this hybridization microchip was able to increase the hybridization
Li-Wei Lai, Qiaole Zhao, Yu-Cheng Lin, Chuan-You Ye, Chuang-Di Chueh, Yung-Chiang Chung   +5 more
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Locked Nucleic Acids and Intercalating Nucleic Acids in the Design of Easily Denaturing Nucleic Acids: Thermal Stability Studies

ChemBioChem, 2004
AbstractIntercalating nucleic acids (INA®s) with insertions of (R)‐1‐O‐(1‐pyrenylmethyl)glycerol were hybridized with locked nucleic acids (LNAs). INA/LNA duplexes were found to be less stable than the corresponding DNA/LNA duplexes when the INA monomer was inserted as a bulge close to the LNA monomers in the opposite strand.
Filichev, V.V., Hilmy, K.M.H., Christensen, Ulf B., Pedersen, Erik Bjerreg., Babu, Bolle Ravindra, Wengel, Jesper   +5 more
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