Results 241 to 250 of about 252,695 (308)

Thermal denaturation of nucleic acids in polyacrylamide gels

Analytical Biochemistry, 1974
Abstract A system is described for measuring thermal denaturation of nucleic acid fractions directly in polyacrylamide gels. Total nucleic acids were fractionated by disc gel electrophoresis. The buffer within the gel was then exchanged for one commonly used in denaturation studies.
Arie Rosner, Marvin Edelman, Jonathan Gressel   +2 more
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Numerical analysis of thermal denaturation of nucleic acids

Analytical Biochemistry, 1978
Denaturation of DNA molecules by stepwise incrementation of the temperature leads to melting profiles showing a fine structure, composed of individual melting modes. A method is described by which quantitative physical information brought by the modes can be extracted from the melting profile.
J. Gabarro
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Denaturation bubble-mediated two-stage isothermal nucleic acid amplification in a single closed tube

Chemical Communications, 2023
A two-stage isothermal amplification method termed rolling cycle strand exchange amplification (RC-SEA) was established to accomplish ssRNA and dsDNA analysis using only one DNA polymerase in a single closed tube at a constant temperature.
Jinling Guo, Yang Li, Qing Wang, Qianqian Jiang, Xinguang Zhang, Peng Zhang, Cuiping Ma, Chao Shi   +7 more
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Effects of cupric ions on thermal denaturation of nucleic acids

Journal of Molecular Biology, 1965
The midpoint of thermal denaturation of sodium DNA and RNA decreases with increasing concentration of cupric ions. With Micrococcus lysodeikticus DNA, the Tm† is 69°C without cupric ions and 36°C with 0·1 m M -cupric ions in 5 m M -sodium chloride. In a solvent consisting of 5 m M -sodium chloride and 0·1 m M -cupric ions, Tm values of several DNA's ...
Susumu Hiai
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Small-volume cuvette for high-resolution thermal denaturation of nucleic acids

Analytical Biochemistry, 1979
Abstract A small-volume cuvette and hoider makes it possible to obtain useful high-resolution thermal denaturation profiles on as little as submicrogram quantities of DNA and routine results with approximately 2 μg of DNA. The reduced sample size is especially important in the thermal characterization of DNA restriction fragments.
Allen T. Ansevin, Douglas L. Vizard
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Theoretical Aspects of Thermal Denaturation of Nucleic Acids

, 1998
Information on mutations (as measured by D/TGGE) can be significantly improved by the use of a theoretical description of the thermal denaturation of nucleic acids.
Michael Etscheid, Detlev Riesner
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Denaturation of Nucleic Acids Induced by Intercalating Agents. Biochemical and Biophysical Properties of Acridine Orange-DNA Complexes

Journal of Biomolecular Structure and Dynamics, 1984
At high binding densities acridine orange (AO) forms complexes with ds DNA which are insoluble in aqueous media. These complexes are characterized by high red- and minimal green-luminescence, 1:1 (dye/P) stoichiometry and resemble complexes of AO with ss nucleic acids.
Jan Kapuściński, Zbigniew Darżynkiewicz   +1 more
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Methods for thermal denaturation studies of nucleic acids in complex with fluorogenic dyes

, 2019
Thermal denaturation is a common technique in the biophysical study of nucleic acids. These experiments are typically performed by monitoring the increase in absorbance (hyperchromism) of a sample at 260nm with temperature (Mergny & Lacroix, 2003; Puglisi & Tinoco, 1989).
Lauren M. Aufdembrink, Tanner G. Hoog, Matthew R. Pawlak, Benjamin F. Bachan, Joseph M. Heili, Aaron E. Engelhart   +5 more
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[Alternating current polarography criteria of nucleic acid denaturation].

Biopolymers, 1967
H, Berg, H, Bär, F A, Gollmick
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Spatiotemporal features of nucleic acid hydration and their changes during denaturation revealed by THz spectroscopy

2015 40th International Conference on Infrared, Millimeter, and Terahertz waves (IRMMW-THz), 2015
Dynamics and structure of water network around nucleic acid have been subjects of intensive studies due to their contributions to biological process. Here, we identified spatiotemporal features of DNA hydration through examining fully-hydrated DNA solutions by THz spectroscopy. The results suggest the presence of weakly bound water beyond tight-binding
Heyjin Son, Jin Young Jeong, Sunmyoung Kim, Jaehun Park, Jeongmin Jang, Kihoon Eom, In‐Kyung Park, Gun‐Sik Park   +7 more
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