Results 251 to 260 of about 323,426 (336)

The reversible denaturation of DNA and its use in studies of nucleic acid homologies and the biological relatedness of microorganisms

open access: closedJournal de Chimie Physique, 1961
Les chaines complementaires de la molecule d’ADN, qui sont liees l’une a l’autre par des liaisons hydrogene au niveau des paires de bases adenine-thymine et guanine-cytosine, peuvent etre separees par l’action de la chaleur, d’un acide, d’une base ou du formamide.
J. Marmur, C. Schildkraut, P. Doty
semanticscholar   +4 more sources

Thermal denaturation of nucleic acids in polyacrylamide gels

open access: closedAnalytical Biochemistry, 1974
Abstract A system is described for measuring thermal denaturation of nucleic acid fractions directly in polyacrylamide gels. Total nucleic acids were fractionated by disc gel electrophoresis. The buffer within the gel was then exchanged for one commonly used in denaturation studies.
Arie Rosner   +2 more
openalex   +4 more sources

Deoxypentose nucleic acids XIV. The denaturation of deoxyribonucleic acid in aqueous solution: properties of DNA denaturated by dilution

open access: closedBiochimica et Biophysica Acta, 1960
Abstract Sedimentation studies indicate that native DNA and DNA denatured by dilution with water below the critical concentration zone have similar sedimentation coefficients and sedimentation coefficient concentration dependence. This is similar to the findings for alkali and thermally denatured DNA.
Ross B. Inman, D. O. Jordan
openalex   +4 more sources

Small-volume cuvette for high-resolution thermal denaturation of nucleic acids

open access: closedAnalytical Biochemistry, 1979
Abstract A small-volume cuvette and hoider makes it possible to obtain useful high-resolution thermal denaturation profiles on as little as submicrogram quantities of DNA and routine results with approximately 2 μg of DNA. The reduced sample size is especially important in the thermal characterization of DNA restriction fragments.
Allen T. Ansevin, Douglas L. Vizard
openalex   +4 more sources

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