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Microfluidic chip of fast DNA hybridization using denaturing and motion of nucleic acids [PDF]
ELECTROPHORESIS, 2008 AbstractThis study presents the effect of fluidic temperatures and velocities on improving DNA hybridization. The efficiency of hybridization could be improved by introducing elevated temperature in the hot region and velocity in the cold region. Compared with the conventional methods, this hybridization microchip was able to increase the hybridization
Li-Wei Lai +5 more
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ChemBioChem, 2004
AbstractIntercalating nucleic acids (INA®s) with insertions of (R)‐1‐O‐(1‐pyrenylmethyl)glycerol were hybridized with locked nucleic acids (LNAs). INA/LNA duplexes were found to be less stable than the corresponding DNA/LNA duplexes when the INA monomer was inserted as a bulge close to the LNA monomers in the opposite strand.
Filichev, V.V. +5 more
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AbstractIntercalating nucleic acids (INA®s) with insertions of (R)‐1‐O‐(1‐pyrenylmethyl)glycerol were hybridized with locked nucleic acids (LNAs). INA/LNA duplexes were found to be less stable than the corresponding DNA/LNA duplexes when the INA monomer was inserted as a bulge close to the LNA monomers in the opposite strand.
Filichev, V.V. +5 more
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Analysis and purification of peptide nucleic acids by denaturing PAGE
ELECTROPHORESIS, 2007AbstractA flexible and convenient protocol for the analysis and purification of peptide nucleic acid (PNA) oligomers and PNA‐peptide chimeras by denaturing PAGE is described. Vertical slab gel electrophoresis, 26% in polyacrylamide and 8 M urea at pH 3, was suitable for analysis of oligomers ranging in size from tetramers (4‐mers) to tetradodecamers ...
Robert H. E. Hudson, David W. Dodd
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Biochimica et Biophysica Acta, 1960
Various electrical properties have been derived from conductivity and electrical transport experiments for aqueous solutions of DNA. The specific conductivity shows a discontinuity when plotted against DNA concentration. Below this critical concentration the material transport number falls rapidly with decreasing concentration.
R.B. Inman, D.O. Jordan
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Various electrical properties have been derived from conductivity and electrical transport experiments for aqueous solutions of DNA. The specific conductivity shows a discontinuity when plotted against DNA concentration. Below this critical concentration the material transport number falls rapidly with decreasing concentration.
R.B. Inman, D.O. Jordan
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Journal of Molecular Biology, 2001
The melting temperature of the poly(dA) . poly(dT) double helix is exquisitely sensitive to salt concentration, and the helix-to-coil transition is sharp. Modern calorimetric instrumentation allows this transition to be detected and characterized with high precision at extremely low duplex concentrations.
Jens Völker +3 more
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The melting temperature of the poly(dA) . poly(dT) double helix is exquisitely sensitive to salt concentration, and the helix-to-coil transition is sharp. Modern calorimetric instrumentation allows this transition to be detected and characterized with high precision at extremely low duplex concentrations.
Jens Völker +3 more
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Fast DNA hybridization chip using denature and motion of nucleic acids
2010 5th International Microsystems Packaging Assembly and Circuits Technology Conference, 2010We demonstrate that the efficiency of DNA hybridization could be improved by introducing elevated temperature in the hot region and higher velocities in the cold region of a microfluidic chip. Compared with the conventional methods, this hybridization microchip was shown to increase the hybridization signal 4.6-fold within 30 minutes using a 1.4 kb ...
Yu-Cheng Lin +5 more
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Chromatography of nucleic acids on hydroxyapatite II. Chromatography of denatured DNA
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1969Abstract The chromatographic behavior of DNA denatured by heat at various temperatures, and reacted or not with formaldehyde, on hydroxyapatite columns has been investigated using both stepwise and gradient elution. The recovery of denatured DNA from the columns and the behavior of the chromatographic fractions upon rechromatography have been studied.
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The Journal of Biochemistry, 1992
A method to convert the conventional denaturing gradient gel electrophoresis into a highly reproducible experimental system was developed. It was based on the following experimental findings; (i) dyes, which are small molecules, do not exhibit mobility changes attributed to their conformational change while nucleic acids do; and (ii) most of the ...
Misa Tsubota +4 more
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A method to convert the conventional denaturing gradient gel electrophoresis into a highly reproducible experimental system was developed. It was based on the following experimental findings; (i) dyes, which are small molecules, do not exhibit mobility changes attributed to their conformational change while nucleic acids do; and (ii) most of the ...
Misa Tsubota +4 more
openaire +3 more sources
The reaction of glyoxal with nucleic acid components
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1973Abstract 1. In the reaction of glyoxal with native DNA of T2 phage at temperatures below its T m modified deoxyguanosine residues are formed. 2. Kinetics of the unwinding of T2 phage DNA suggest that the reaction of glyoxal with native DNA results in fixation of the transiently-open regions of the bihelical structure and in further ...
E.J. Budowsky, N.E. Broude
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Analytical and Bioanalytical Chemistry, 2007
Monitoring of in-vitro selection experiments is crucial in evaluation of the success and outcome of such approaches. Furthermore, monitoring running in parallel with the selection procedure enables early intervention and adjustment of stringency to achieve the desired activities of the selected nucleic acid species.
Müller, Jens +4 more
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Monitoring of in-vitro selection experiments is crucial in evaluation of the success and outcome of such approaches. Furthermore, monitoring running in parallel with the selection procedure enables early intervention and adjustment of stringency to achieve the desired activities of the selected nucleic acid species.
Müller, Jens +4 more
openaire +4 more sources