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Monte Carlo applications to thermal and chemical denaturation experiments of nucleic acids and proteins

open access: closed, 2000
Publisher Summary This chapter describes the Monte Carlo applications to thermal and chemical denaturation experiments of nucleic acids and proteins. Information about the states of systems must often be extracted indirectly, from the measurements of properties, considered characteristic of these states.
D. Jeremy Williams, Kathleen B. Hall
openalex   +3 more sources

Features of Determining Thermodynamic Parameters of Formation of Nucleic Acid Complexes Using Thermal Denaturation with Fluorimetric Signal Detection

open access: closedRussian journal of bioorganic chemistry, 2019
Georgiy Yu. Shevelev   +4 more
openalex   +2 more sources
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Isothermal Detection of Canine Blood Parasite (Ehrlichia canis) Utilizing Recombinase Polymerase Amplification Coupled with Graphene Oxide Quenching-Based Pyrrolidinyl Peptide Nucleic Acid.

Bioconjugate chemistry, 2021
Canine monocytic ehrlichiosis (CME), caused by transmitted Ehrlichia canis infection, is a major disease in dogs with worldwide distribution. Herein, a nucleic acid assay was established for the identification of E.
Phanomsak Yukhet   +3 more
semanticscholar   +1 more source

Locked Nucleic Acids and Intercalating Nucleic Acids in the Design of Easily Denaturing Nucleic Acids: Thermal Stability Studies

ChemBioChem, 2004
AbstractIntercalating nucleic acids (INA®s) with insertions of (R)‐1‐O‐(1‐pyrenylmethyl)glycerol were hybridized with locked nucleic acids (LNAs). INA/LNA duplexes were found to be less stable than the corresponding DNA/LNA duplexes when the INA monomer was inserted as a bulge close to the LNA monomers in the opposite strand.
Filichev, V.V.   +5 more
openaire   +3 more sources

Analysis and purification of peptide nucleic acids by denaturing PAGE

ELECTROPHORESIS, 2007
AbstractA flexible and convenient protocol for the analysis and purification of peptide nucleic acid (PNA) oligomers and PNA‐peptide chimeras by denaturing PAGE is described. Vertical slab gel electrophoresis, 26% in polyacrylamide and 8 M urea at pH 3, was suitable for analysis of oligomers ranging in size from tetramers (4‐mers) to tetradodecamers ...
David W, Dodd, Robert H E, Hudson
openaire   +2 more sources

Spherical Nucleic Acid Probes on Floating-Gate Field-Effect Transistor Biosensors for Attomolar-Level Analyte Detection.

ACS Nano
Field-effect transistor (FET) sensors are attractive for the label-free detection of target biomolecules, offering ultrahigh sensitivity and a rapid response.
Haoran Wang   +7 more
semanticscholar   +1 more source

Pyridine: a Denaturant or Stabilizer of Spherical Nucleic Acids?

Analytical Chemistry, 2017
The spotlighted dual functions of pyridine as a denaturant and as a stabilizer for duplex DNA are thoroughly investigated using spherical nucleic acids (SNAs). At neutral pH, pyridine destabilizes the duplex interconnects of assembled SNAs, resulting in a gradual decrease in their melting temperature (Tm) as a function of the pyridine concentration ...
Yoon Hyuck Kim   +3 more
openaire   +2 more sources

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