Results 261 to 270 of about 252,695 (308)

[Protective action of nucleic acids on denaturation of ovalbumin].

Bollettino della Societa italiana di biologia sperimentale, 2000
R. Rendi
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Microfluidic chip of fast DNA hybridization using denaturing and motion of nucleic acids

ELECTROPHORESIS, 2008
AbstractThis study presents the effect of fluidic temperatures and velocities on improving DNA hybridization. The efficiency of hybridization could be improved by introducing elevated temperature in the hot region and velocity in the cold region. Compared with the conventional methods, this hybridization microchip was able to increase the hybridization
Yung-Chiang, Chung, Yu-Cheng, Lin, Chuang-Di, Chueh, Chuan-You, Ye, Li-Wei, Lai, Qiaole, Zhao   +5 more
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Fast DNA hybridization chip using denature and motion of nucleic acids

2010 5th International Microsystems Packaging Assembly and Circuits Technology Conference, 2010
We demonstrate that the efficiency of DNA hybridization could be improved by introducing elevated temperature in the hot region and higher velocities in the cold region of a microfluidic chip. Compared with the conventional methods, this hybridization microchip was shown to increase the hybridization signal 4.6-fold within 30 minutes using a 1.4 kb ...
Yung-Chiang Chung, Yu-Cheng Lin, Chaung-Di Chueh, Chuan-You Ye, Li-Wei Lai, Wei-Chieh Liao   +5 more
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Chromatography of nucleic acids on hydroxyapatite II. Chromatography of denatured DNA

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1969
Abstract The chromatographic behavior of DNA denatured by heat at various temperatures, and reacted or not with formaldehyde, on hydroxyapatite columns has been investigated using both stepwise and gradient elution. The recovery of denatured DNA from the columns and the behavior of the chromatographic fractions upon rechromatography have been studied.
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Counterion association with native and denatured nucleic acids: an experimental approach.

Journal of molecular biology, 2001
The melting temperature of the poly(dA) . poly(dT) double helix is exquisitely sensitive to salt concentration, and the helix-to-coil transition is sharp. Modern calorimetric instrumentation allows this transition to be detected and characterized with high precision at extremely low duplex concentrations.
J, Völker, H H, Klump, G S, Manning, K J, Breslauer   +3 more
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Influence of adsorbability of denatured deoxyribonucleic acid on its polarographic reducibility

Journal of Electroanalytical Chemistry, 1974
Summary The polarographic reduction of denatured DNA takes place in the adsorbed state. Reduction polarographic currents of denatured DNA in those pH regions, where the current does not change much with pH, have the character of adsorption currents under conditions when the surface of mercury electrode is fully covered by adsorbed polynucleotide ...
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Integrated silica membrane–based nucleic acid purification, amplification, and visualization platform for low-cost, rapid detection of foodborne pathogens

Analytical and Bioanalytical Chemistry, 2020
Xiudan Wang, Chunyu Yan, Xiaokun Wang, Xiaoli Zhao, Chao Shi, Cuiping Ma   +5 more
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Structural analysis of nucleic acids by precise denaturing gradient gel electrophoresis: I. Methodology.

Journal of biochemistry, 1992
A method to convert the conventional denaturing gradient gel electrophoresis into a highly reproducible experimental system was developed. It was based on the following experimental findings; (i) dyes, which are small molecules, do not exhibit mobility changes attributed to their conformational change while nucleic acids do; and (ii) most of the ...
K, Nishigaki, M, Tsubota, T, Miura, Y, Chonan, Y, Husimi   +4 more
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Nucleic Acid Extraction from Human Biological Samples.

Methods in molecular biology, 2018
S. Mullegama, M. O. Alberti, Cora Au, Yan Li, Traci Toy, Vanina Tomasian, Rena R. Xian   +6 more
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One-Dimensional Electrophoresis of Nucleic Acids in Agarose Using Denaturation with Formaldehyde and Identification of <SUP>3</SUP>H-Labeled RNA by Fluorography

, 2003
Theodore Gurney
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