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Numerical analysis of thermal denaturation of nucleic acids
Analytical Biochemistry, 1978 Denaturation of DNA molecules by stepwise incrementation of the temperature leads to melting profiles showing a fine structure, composed of individual melting modes. A method is described by which quantitative physical information brought by the modes can be extracted from the melting profile.
J. Gabarro
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Effects of cupric ions on thermal denaturation of nucleic acids
Journal of Molecular Biology, 1965 The midpoint of thermal denaturation of sodium DNA and RNA decreases with increasing concentration of cupric ions. With Micrococcus lysodeikticus DNA, the Tm† is 69°C without cupric ions and 36°C with 0·1 m M -cupric ions in 5 m M -sodium chloride. In a solvent consisting of 5 m M -sodium chloride and 0·1 m M -cupric ions, Tm values of several DNA's ...
Susumu Hiai
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Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1973
Abstract 1. In the reaction of glyoxal with native DNA of T2 phage at temperatures below its T m modified deoxyguanosine residues are formed. 2. Kinetics of the unwinding of T2 phage DNA suggest that the reaction of glyoxal with native DNA results in fixation of the transiently-open regions of the bihelical structure and in further ...
N. Broude, E. I. Budowsky
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Abstract 1. In the reaction of glyoxal with native DNA of T2 phage at temperatures below its T m modified deoxyguanosine residues are formed. 2. Kinetics of the unwinding of T2 phage DNA suggest that the reaction of glyoxal with native DNA results in fixation of the transiently-open regions of the bihelical structure and in further ...
N. Broude, E. I. Budowsky
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, 2000 Publisher Summary This chapter describes the Monte Carlo applications to thermal and chemical denaturation experiments of nucleic acids and proteins. Information about the states of systems must often be extracted indirectly, from the measurements of properties, considered characteristic of these states.
D. Jeremy Williams, Kathleen B. Hall
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Biochimica et Biophysica Acta, 1960
Various electrical properties have been derived from conductivity and electrical transport experiments for aqueous solutions of DNA. The specific conductivity shows a discontinuity when plotted against DNA concentration. Below this critical concentration the material transport number falls rapidly with decreasing concentration.
R. Inman, D. O. Jordan
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Various electrical properties have been derived from conductivity and electrical transport experiments for aqueous solutions of DNA. The specific conductivity shows a discontinuity when plotted against DNA concentration. Below this critical concentration the material transport number falls rapidly with decreasing concentration.
R. Inman, D. O. Jordan
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Denaturation bubble-mediated two-stage isothermal nucleic acid amplification in a single closed tube
Chemical Communications, 2023 A two-stage isothermal amplification method termed rolling cycle strand exchange amplification (RC-SEA) was established to accomplish ssRNA and dsDNA analysis using only one DNA polymerase in a single closed tube at a constant temperature.
Jinling Guo +7 more
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Theoretical Aspects of Thermal Denaturation of Nucleic Acids
, 1998 Information on mutations (as measured by D/TGGE) can be significantly improved by the use of a theoretical description of the thermal denaturation of nucleic acids.
Michael Etscheid, Detlev Riesner
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Methods for thermal denaturation studies of nucleic acids in complex with fluorogenic dyes
, 2019 Thermal denaturation is a common technique in the biophysical study of nucleic acids. These experiments are typically performed by monitoring the increase in absorbance (hyperchromism) of a sample at 260nm with temperature (Mergny & Lacroix, 2003; Puglisi & Tinoco, 1989).
Lauren M. Aufdembrink +5 more
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Biochimica et Biophysica Acta, 1960 Evidence has been reported which indicates that dilution of a salt free DNA solution below a critical concentration zone (4–50 · 10−5M) results in denaturation. This process is irreversible with respect to the specific hydrogen bonds present in the native structure, but is thought to be partially reversible with respect to non-specific hydrogen bonds ...
Ross B. Inman, D. O. Jordan
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2015 40th International Conference on Infrared, Millimeter, and Terahertz waves (IRMMW-THz), 2015 Dynamics and structure of water network around nucleic acid have been subjects of intensive studies due to their contributions to biological process. Here, we identified spatiotemporal features of DNA hydration through examining fully-hydrated DNA solutions by THz spectroscopy. The results suggest the presence of weakly bound water beyond tight-binding
Heyjin Son +7 more
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