Results 61 to 70 of about 5,943,194 (262)

Optimization of seminal extracellular vesicle isolation protocols for cancer biomarker analysis using proteomic profiling

Molecular Oncology, EarlyView.
This paper shows that size‐exclusion chromatography and sequential centrifugation applied to seminal plasma enrich for different populations of extracellular vesicles. This may significantly affect the detection and analysis of extracellular vesicle‐associated cancer biomarkers.
Elen Telumyan, Beatriz Martín‐Gracia, Eva Bassols‐Citores, Krizia Sagini, Maria K. Andersen, Tuula A. Nyman, May‐Britt Tessem, Morten B. Rye, Alicia Llorente   +8 more
wiley   +1 more source

Loop-mediated Isothermal Amplification (LAMP): Current progress and future directions in rapid nucleic acid detection

Sensors and Actuators Reports
Following the COVID-19 pandemic, there has been an escalating demand for rapid and precise disease diagnostics. Nucleic acid detection technology, owing to its exceptional specificity and sensitivity, has increasingly gained prominence in disease ...
Yifan Yang, Ruolin Yang, Chengkun Luo, Xuefeng Wang, Fuqiang Ma   +4 more
doaj   +1 more source

Locked Nucleic Acid (LNA): An Improved Alternative to DNA for Nanoscale ‘On-Surface’ Nucleic Acid Detection [PDF]

, 2019
The research work embodied in the present thesis entitled “Locked Nucleic Acid (LNA): An Improved Alternative to DNA for Nanoscale ‘On-Surface’ Nucleic Acid Detection” deals with the effectiveness of the locked nucleic acid (LNA) probe, which is ...
Mishra, Sourav
core  

Studies on the relation between heme and nucleic acid syntheses in erythroid cell. II. Nucleic acid synthesis in erythroblast of anemic rat treated with aminopterin and bromouracil [PDF]

, 1968
With the bone marrow of anemic rats, which had received the repeated injections of phenylhydrazine once a day for three to four days, the effects of aminopterin and bromouracil on the nucleic acid metabolism of erythroblasts were observed in vivo ...
Shigehisa, Morio
core   +1 more source

Attitudes of cancer patients toward ctDNA‐based liquid biopsy for follow‐up

Molecular Oncology, EarlyView.
This mixed‐methods study explored cancer patients' experiences, knowledge, and preferences regarding ctDNA‐based liquid biopsy for follow‐up. Awareness of ctDNA was low and recall of previous liquid biopsy was imperfect, yet acceptance was high.
Siddharth Chhajlani, Laura Mariën, Caro De Weerdt, Sanne Wouters, Laure‐Anne Teuwen, Hans Prenen, Katleen Janssens, Timon Vandamme   +7 more
wiley   +1 more source

Discovery of mpox virus nucleic acid in bile: a case with multiple coinfections

International Journal of Infectious Diseases
We report the detection of Monkeypox virus (MPXV) nucleic acid in bile from a rash-free patient with advanced HIV and multiple coinfections. This case raises the possibility that MPXV nucleic acid may be detectable in bile and represents, to our ...
Mengze Gan, Jinmei Qin, Xiaoying Qin, Renping Peng, Qiuye Xiao, Shuhui Wang, Yinduo Wei, Liuying Wei, Rui Zhao, Zhouhua Xie, Dan Li, Jun Zou   +11 more
doaj   +1 more source

Application of CRISPR/Cas Systems in the Nucleic Acid Detection of Infectious Diseases

, 2022
The CRISPR/Cas system is a protective adaptive immune system against attacks from foreign mobile genetic elements. Since the discovery of the excellent target-specific sequence recognition ability of the CRISPR/Cas system, the CRISPR/Cas system has shown
Bin Wang, Yuefei Jin, Peng Ni, Wang, Yuexia, Junwei Li, Li, Junwei, Zhang, Rongguang, Wang, Bin, Duan, Guangcai, Ni, Peng, Lou, Juan, Guangcai Duan, Juan Lou, Jin, Yuefei, Yuexia Wang, Rongguang Zhang, Shuaiyin Chen, Chen, Shuaiyin   +17 more
core   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

FEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová, Grigorii Rakhalskii, Miroslav Almáši   +2 more
wiley   +1 more source

Rapid Preparation Reagent for RPA-LFD Detection of DNA Samples from the Shrimp Hepatopancreas

Progress in Fishery Sciences
This study is committed to developing and optimizing a rapid nucleic acid preparation reagent (nucleic acid release agent) that is suitable for recombinase polymerase amplification with lateral flow dipstick (RPA-LFD) technology to detect DNA nucleic ...
Mengli YAO, Changming BAI, Chongming WANG, Lusheng XIN   +3 more
doaj   +1 more source