Results 61 to 70 of about 5,589,910 (303)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Designed diblock hairpin probes for the nonenzymatic and label-free detection of nucleic acid

, 2016
The detection of nucleic acid sequences is of great importance in a variety of fields. An ultrasensitive DNA sensing platform is constructed using elaborately designed diblock hairpin probes (DHPs) that are composed of hairpin and poly-adenine blocks ...
Wen, Junlin, Zhou, Shungui, Chen, Junhua, Zhuang, Li   +3 more
core   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

Developing nucleic acid-based electrical detection systems [PDF]

, 2006
Development of nucleic acid-based detection systems is the main focus of many research groups and high technology companies. The enormous work done in this field is particularly due to the broad versatility and variety of these sensing devices.
Gabig-Cimińska, Magdalena
core   +2 more sources

Loop-mediated Isothermal Amplification (LAMP): Current progress and future directions in rapid nucleic acid detection

Sensors and Actuators Reports
Following the COVID-19 pandemic, there has been an escalating demand for rapid and precise disease diagnostics. Nucleic acid detection technology, owing to its exceptional specificity and sensitivity, has increasingly gained prominence in disease ...
Yifan Yang, Ruolin Yang, Chengkun Luo, Xuefeng Wang, Fuqiang Ma   +4 more
doaj   +1 more source

Nucleic Acid (Patent US 2009/0019559 A1) [PDF]

, 2009
The invention relates to nucleic acid derived from Perkinsus marinus which encodes a 9-elongase, a Delta8-desaturase and a Delta5-desaturase enzyme.
Sayanova, O. V., Caleron, M. V., Napier, J. A.   +2 more
core  

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

FEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová, Grigorii Rakhalskii, Miroslav Almáši   +2 more
wiley   +1 more source

Rapid Preparation Reagent for RPA-LFD Detection of DNA Samples from the Shrimp Hepatopancreas

Progress in Fishery Sciences
This study is committed to developing and optimizing a rapid nucleic acid preparation reagent (nucleic acid release agent) that is suitable for recombinase polymerase amplification with lateral flow dipstick (RPA-LFD) technology to detect DNA nucleic ...
Mengli YAO, Changming BAI, Chongming WANG, Lusheng XIN   +3 more
doaj   +1 more source

Nucleic Acid (Patent WO 2007/093776 A2) [PDF]

, 2007
The invention relates to nucleic acid derived from Perkinsus marinus which encodes a 9-elongase, a ?8-desaturase and a ?5-desaturase enzyme.
Sayanova, O. V., Napier, J. A., Venegas, C. M.   +2 more
core  

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

FEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose, Kotaro Ozaki, Ryohei Kuriyama, Yuka Ikemoto, Kazuhiro Mio, Yuji C. Sasaki, Tatsuya Arai, Sakae Tsuda, Yoichi Shinkai, Masahiro Kuramochi   +9 more
wiley   +1 more source