Results 81 to 90 of about 5,589,910 (303)

Nucleic Acid Detection of Plant Genes Using CRISPR-Cas13

, 2021
Nucleic acid detection is vital for agricultural applications including trait detection during breeding, pest surveillance, and pathogen identification.
Abudayyeh, Omar O, Kellner, Max J., Abudayyeh, Omar O., Gootenberg, Jonathan S, Kellner, Max J, Zhang, Feng   +5 more
core   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Heterotropic regulation and negative homotropic cooperativity

FEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea, Francesco Angelucci, Federica Arnesano, Gianmarco Pascarella, Patrizio Di Micco, Andrea Bellelli   +5 more
wiley   +1 more source

Single- and dual- near-infrared fluorescent labeled nucleic acid conjugate for nucleic acid detection

, 2001
In this research, a near-IR fluorescent labeled nucleic acid conjugate for the nucleic acid detection was synthesized, and characterized preliminarily for the detection of the nucleic acid.
Zhihong Lin, Arbter, Michaela, Boehmer, Martin, Vladimir Mirsky, Shu Ren, Mirsky, Vladimir, Wolfbeis, Otto S., Martin Boehmer, Otto S. Wolfbeis, Michaela Arbter, Lin, Zhihong, Ren, Shu, Meng Wu, Wu, Meng   +13 more
core   +1 more source

Nucleic acids and protein synthesis in cancer cell mitochondria. I. Nucleic acids in rat hepatoma mitochondria [PDF]

, 1967
The contents of nucleic acids in rat liver and hepatoma mitochondria and the physico-chemical properties on DNA's isolated from these mitochondria were comparatively investigated. The results are briefly summarized as follows. 1.
Inaba, Kozo
core   +1 more source

Using cell‐free RNA to identify B‐ and T‐cell clonality for diagnosis and monitoring of B‐ and T‐cell neoplasms

FEBS Open Bio, EarlyView.
Using peripheral blood for determining B‐cell or T‐cell clonality is more reliable when we use cell‐free RNA (cfRNA) because cells release blood significantly more RNA than DNA. Next‐generation sequencing (NGS) of cfRNA allows us to evaluate fragment cfRNA and evaluate clonality reliably without the need for prior determination of the specific dominant
Adam Albitar, Sally Agersborg, Ahmed Charifa, Pooja Phull, Noa Biran, David Vesole, Harsh Parmar, Andrew Pecora, Andrew Ip, Andre Goy, David Siegel, Maher Albitar   +11 more
wiley   +1 more source

Establishment and preliminary verification of a highly sensitive nucleic acid extraction method for OBI detection

Zhongguo shuxue zazhi
Objective To establish and verify a new nucleic acid extraction method for OBI detection with large volume and high sensitivity, and apply it in the quantitative determination of OBI samples with low viral load.
Wenbo GAO, Bo HE, Rongsong DU, Fenfang LIAO, Junmou XIE, Min WANG, Hao WANG   +6 more
doaj   +1 more source

Research progress of Class 2 CRISPR-Cas system in nucleic acid detection of animal pathogens [PDF]

Frontiers of Agricultural Science and Engineering
The rapid spread of animal diseases and the evolution of associated pathogens underscore the urgent need for improved diagnostic techniques. Established nucleic acid detection methods typically rely on expensive and complex machinery, which requires ...
Shuai ZHANG, Xi SHEN, Zhongzhi LIU, Debao HU, Xin LI, Yiwen GUO, Xiangbin DING, Linlin ZHANG   +7 more
doaj   +1 more source

Comparison of different real-time PCR chemistries and their suitability for detection and quantification of genetically modified organisms [PDF]

, 2008
Background The real-time polymerase chain reaction is currently the method of choice for quantifying nucleic acids in different DNA based quantification applications. It is widely used also for detecting and quantifying genetically modified components in
Žel Jana, Cankar, K., Cankar Katarina, Buh Gašparič Meti, Buh Gašparič, Meti, Gasparic, M.B., Gruden Kristina, Gruden, Kristina, Cankar, Katarina, Gruden, K., Zel, J., Žel, Jana   +11 more
core   +1 more source

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

FEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer, Pawel Rubach, Zbigniew Dauter, Wojciech Dec, Dariusz Brzezinski, Marta Kulik, Wladek Minor, Mariusz Jaskolski   +7 more
wiley   +1 more source