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Nucleic Acid Hybridization to Isolated Chromatin
1972Three procedures are in general use for the formation and detection of RNA-DNA hybrids: reactions on a filter (Gillespie and Spiegelman, 1965); reaction in liquid (Nygaard and Hall, 1963); and reaction in agar (McCarthy and Bolton, 1964). The inherent limitations of each procedure have been studied in detail (Kennell and Kotoulas, 1968). Because of its
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Hybridization of nucleic acids directly in agarose gels
Analytical Biochemistry, 1983Nucleic acids, both DNA and RNA, separated on agarose gels can be visualized by direct hybridization of the dried gel with appropriate radioactive probes. This method does not involve the transfer of the nucleic acid from the gel. The method requires less manipulation than other procedures; it is extremely rapid, sensitive, and inexpensive.
S G, Tsao, C F, Brunk, R E, Pearlman
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Principles and Practices of Nucleic Acid Hybridization
1971Publisher Summary This chapter discusses that nucleic acid hybrid formation has been used as a research tool for approximately one decade. It has been a decade of remarkable progress toward understanding mechanisms of gene expression. Probably no other single procedure has contributed more to this success than has hybridization.
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Nucleic Acid Hybridization for the Detection of Viral Genomes
1983Single-stranded nucleic acids can form stable duplexes if they encounter complementary sequences under appropriate conditions. This reaction is called hybridization and has been extensively used during the past 15 years to explore the structure and expression of cellular and viral genes. Particularly with the advance of molecular cloning, hybridization
G W, Bornkamm +2 more
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Nucleic Acid Hybridization Procedures
2019The application of nucleic acid probes and related techniques is becoming common in the detection of human and other animal viruses but has lagged somewhat in plant virology. The explosion in the use of the technology associated with recombinant DNA research has led to the development of various techniques by which viruses can be diagnosed.
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Significance of Nucleic Acid Hybridization to Systematics of Actinomycetes
1975Publisher Summary This chapter discusses the systematic of actinomycetes, along with the significance of nucleic acid hybridization to it. Systematics progresses through three stages: the description of cultures leading to bases for distinguishing between strains; the development of keys, classification schemes, and a system of nomenclature; and an ...
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DNA Probes: Applications of the Principles of Nucleic Acid Hybridization
Critical Reviews in Biochemistry and Molecular Biology, 1991James G Wetmur
exaly