Results 31 to 40 of about 316,767 (328)

Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization.

PCR methods and applications, 1995
The 5' nuclease PCR assay detects the accumulation of specific PCR product by hybridization and cleavage of a double-labeled fluorogenic probe during the amplification reaction.
Genome Research, Kenneth J. Livak, Susan J.A. Flood, Jeffrey Marmaro, William Giusti, Karin Deetz   +5 more
semanticscholar   +1 more source

A Widely Applicable Urea-based Fluorescent/Colorimetric mRNA in situ Hybridization Protocol

Bio-Protocol, 2019
In situ hybridization methods are routinely employed to detect nucleic acid sequences, allowing to localize gene expression or to study chromosomal organization in their native context. These methods rely on the pairwise binding of a labeled probe to the
Chiara Sinigaglia
doaj   +1 more source

Effect of Native Gastric Mucus on in vivo Hybridization Therapies Directed at Helicobacter pylori

Molecular Therapy: Nucleic Acids, 2015
Helicobacter pylori infects more than 50% of the worldwide population. It is mostly found deep in the gastric mucus lining of the stomach, being a major cause of peptic ulcers and gastric adenocarcinoma. To face the increasing resistance of H.
Rita S Santos, George R Dakwar, Ranhua Xiong, Katrien Forier, Katrien Remaut, Stephan Stremersch, Nuno Guimarães, Sílvia Fontenete, Jesper Wengel, Marina Leite, Céu Figueiredo, Stefaan C De Smedt, Kevin Braeckmans, Nuno F Azevedo   +13 more
doaj   +1 more source

Influence of citrate buffer and flash heating in enhancing the sensitivity of ratiometric genosensing of Hepatitis C virus using plasmonic gold nanoparticles

Micro and Nano Systems Letters, 2021
Gold nanoparticles (Au NPs) based technology has been shown to possess enormous potential in the viral nucleic acid diagnosis. Despite significant advancement in this domain, the existing literature reveals the diversity in the conditions employed for ...
Hrishikesh Shashi Prakash, Pranay Amruth Maroju, Naga Sai Sriteja Boppudi, Aniket Balapure, Ramakrishnan Ganesan, Jayati Ray Dutta   +5 more
doaj   +1 more source

Universal Dynamic DNA Assembly-Programmed Surface Hybridization Effect for Single-Step, Reusable, and Amplified Electrochemical Nucleic Acid Biosensing.

Analytical Chemistry, 2017
The traditional sensitive electrochemical biosensors are commonly confronted with the cumbersome interface operation and washing procedures and the inclusion of extra exogenous reagents, which impose the challenge on the detection simplicity, reliability,
Shufeng Liu, Li-hong Fang, Yanqun Wang, Li Wang   +3 more
semanticscholar   +1 more source

Penggunaan Pelacak DNA untuk Deteksi Papaya ringspot virus dengan Metode Hibridisasi Asam Nukleat

Jurnal Fitopatologi Indonesia, 2018
Use of DNA Probe for Detection of Papaya ringspot virus Using Nucleic Acid Hybridization MethodPapaya ringspot caused by Papaya ringspot virus (PRSV) is one of the most destructive diseases of papaya.
Irsan Nuhantoro, Sri Hendrastuti Hidayat, Kikin Hamzah Mutaqin   +2 more
doaj   +1 more source

Thermodynamic framework to assess low abundance DNA mutation detection by hybridization. [PDF]

PLoS ONE, 2017
The knowledge of genomic DNA variations in patient samples has a high and increasing value for human diagnostics in its broadest sense. Although many methods and sensors to detect or quantify these variations are available or under development, the ...
Hanny Willems, An Jacobs, Wahyu Wijaya Hadiwikarta, Tom Venken, Dirk Valkenborg, Nadine Van Roy, Jo Vandesompele, Jef Hooyberghs   +7 more
doaj   +1 more source

Nucleic acid analysis by sandwich hybridization

Journal of Clinical Laboratory Analysis, 1989
AbstractOne of the most significant achievements of the biochemist during the past two decades is the use to which immunologically based assays have been put in clinical diagnosis (Hood et al.: Immunology, 1984). The problem faced and surmounted by immunologists in effecting the transition from research tool to routine clinical assay bears a remarkable
Peter Nicholls, Alan D. B. Malcolm
openaire   +2 more sources

Detection of target DNA using fluorescent cationic polymer and peptide nucleic acid probes on solid support

BMC Biotechnology, 2005
Background Nucleic acids detection using microarrays requires labelling of target nucleic acids with fluorophores or other reporter molecules prior to hybridization.
Leclerc Mario, Picard François J, Boissinot Maurice, Bissonnette Luc, Peytavi Régis, Ho Hoang-Anh, Raymond Frédéric R, Bergeron Michel G   +7 more
doaj   +1 more source

Hybridization properties of immobilized nucleic acids

Nucleic Acids Research, 1987
The 5'-end attachment of oligonucleotides to dextran supports facilitates the study of the hybridization properties of an immobilized oligonucleotide system. The hybridization properties which were studied include: hybridization capacity and kinetics, hybridization-complex stability, and reagents influencing hybridization efficiency.
G. R. Davis, D.Y. Kwoh, Thomas R. Gingeras   +2 more
openaire   +4 more sources