Results 31 to 40 of about 225,030 (341)

A Widely Applicable Urea-based Fluorescent/Colorimetric mRNA in situ Hybridization Protocol

open access: yesBio-Protocol, 2019
In situ hybridization methods are routinely employed to detect nucleic acid sequences, allowing to localize gene expression or to study chromosomal organization in their native context. These methods rely on the pairwise binding of a labeled probe to the
Chiara Sinigaglia
doaj   +1 more source

Locked nucleic acid pentamers as universal PCR primers for genomic DNA amplification. [PDF]

open access: yesPLoS ONE, 2008
BACKGROUND: Multiplexing technologies, which allow for simultaneous detection of multiple nucleic acid sequences in a single reaction, can save a lot of time, cost and labor compared to traditional single reaction detection methods.
Zhen Sun   +7 more
doaj   +1 more source

The T-cell oncogenic protein HOX11 activates Aldh1 expression in NIH 3T3 cells but represses its expression in mouse spleen development [PDF]

open access: yes, 1998
Hox11 is a homeobox gene essential for spleen formation in mice, since atrophy of the anlage of a developing spleen occurs in early embryonic development in Hox11 null mice.
Greene, W.K.   +3 more
core   +2 more sources

Penggunaan Pelacak DNA untuk Deteksi Papaya ringspot virus dengan Metode Hibridisasi Asam Nukleat

open access: yesJurnal Fitopatologi Indonesia, 2018
Use of DNA Probe for Detection of Papaya ringspot virus Using Nucleic Acid Hybridization MethodPapaya ringspot caused by Papaya ringspot virus (PRSV) is one of the most destructive diseases of papaya.
Irsan Nuhantoro   +2 more
doaj   +1 more source

Advancing transcriptome platforms [PDF]

open access: yes, 2011
During the last decade of years, remarkable technological innovations have emerged that allow the direct or indirect determination of the transcriptome at unprecedented scale and speed.
Shuobo Shi
core   +2 more sources

Thermodynamic framework to assess low abundance DNA mutation detection by hybridization. [PDF]

open access: yesPLoS ONE, 2017
The knowledge of genomic DNA variations in patient samples has a high and increasing value for human diagnostics in its broadest sense. Although many methods and sensors to detect or quantify these variations are available or under development, the ...
Hanny Willems   +7 more
doaj   +1 more source

Detection of monosomy 7 in interphase cells of patients with myeloid disorders [PDF]

open access: yes, 1990
Six patients, five with acute myeloid leukemia (AML) and one with a myelodysplastic syndrome (MDS), were found to have monosomy 7 by conventional cytogenetics at diagnosis.
Cremer, Thomas   +5 more
core   +1 more source

Influence of citrate buffer and flash heating in enhancing the sensitivity of ratiometric genosensing of Hepatitis C virus using plasmonic gold nanoparticles

open access: yesMicro and Nano Systems Letters, 2021
Gold nanoparticles (Au NPs) based technology has been shown to possess enormous potential in the viral nucleic acid diagnosis. Despite significant advancement in this domain, the existing literature reveals the diversity in the conditions employed for ...
Hrishikesh Shashi Prakash   +5 more
doaj   +1 more source

Detection of target DNA using fluorescent cationic polymer and peptide nucleic acid probes on solid support

open access: yesBMC Biotechnology, 2005
Background Nucleic acids detection using microarrays requires labelling of target nucleic acids with fluorophores or other reporter molecules prior to hybridization.
Leclerc Mario   +7 more
doaj   +1 more source

Nanostructured luminescently labeled nucleic acids [PDF]

open access: yes, 2017
Important and emerging trends at the interface of luminescence, nucleic acids and nanotechnology are: (i) the conventional luminescence labeling of nucleic acid nanostructures (e.g. DNA tetrahedron); (ii) the labeling of bulk nucleic acids (e.g. single‐
Adleman   +105 more
core   +1 more source

Home - About - Disclaimer - Privacy