Results 31 to 40 of about 114,253 (306)

Detection of target DNA using fluorescent cationic polymer and peptide nucleic acid probes on solid support

BMC Biotechnology, 2005
Background Nucleic acids detection using microarrays requires labelling of target nucleic acids with fluorophores or other reporter molecules prior to hybridization.
Leclerc Mario, Picard François J, Boissinot Maurice, Bissonnette Luc, Peytavi Régis, Ho Hoang-Anh, Raymond Frédéric R, Bergeron Michel G   +7 more
doaj   +1 more source

Supramolecular Architectures of Nucleic Acid/Peptide Hybrids [PDF]

International Journal of Molecular Sciences, 2020
Supramolecular architectures that are built artificially from biomolecules, such as nucleic acids or peptides, with structural hierarchical orders ranging from the molecular to nano-scales have attracted increased attention in molecular science research fields.
Sayuri L. Higashi, Normazida Rozi, Sharina Abu Hanifah, Masato Ikeda   +3 more
openaire   +2 more sources

Immobilon™-Ny+ Nucleic Acid Blotting Membrane: An Advanced Nylon Membrane Optimized for Superior Fixation and Reprobing

BioTechniques, 1999
The use of charged nylon membranes in nucleic acid blotting applications has become an important factor in the success of hybridization-based assays. Retention of nucleic acids on these membranes is promoted by baking at 80°C under vacuum or by exposure ...
Michael A. Mansfield, Masaharu Mabuchi, Constance G. MacDonald, Malcolm G. Pluskal   +3 more
doaj   +1 more source

The Application of Hybridization Chain Reaction in the Detection of Foodborne Pathogens

Foods, 2023
Today, with the globalization of the food trade progressing, food safety continues to warrant widespread attention. Foodborne diseases caused by contaminated food, including foodborne pathogens, seriously threaten public health and the economy.
Jinbin Zhao, Yulan Guo, Xueer Ma, Shitong Liu, Chunmeng Sun, Ming Cai, Yuyang Chi, Kun Xu   +7 more
doaj   +1 more source

Peach latent mosaic viroid in naturally infected sweet cherry trees in southern Italy

International Journal of Horticultural Science, 2002
Peach latent mosaic viroid (PLMVd) was found in naturally infected sweet cherry trees grown in commercial orchards in southern Italy. The viroid was detected in nucleic acid extracts of symptomless leaves by molecular hybridization with a PLMVd cRNA ...
A. Crescenzi, A. Piazzolla, A. Hadidi
doaj   +1 more source

Bridging the gap: Multi‐stakeholder perspectives of molecular diagnostics in oncology

Molecular Oncology, EarlyView.
Although molecular diagnostics is transforming cancer care, implementing novel technologies remains challenging. This study identifies unmet needs and technology requirements through a two‐step stakeholder involvement. Liquid biopsies for monitoring applications and predictive biomarker testing emerge as key unmet needs. Technology requirements vary by
Jorine Arnouts, Senada Koljenović, Elise Daems, Karolien De Wael, Marc Peeters, Léon C. van Kempen, Greetje Vanhoutte, Karen Zwaenepoel, Timon Vandamme   +8 more
wiley   +1 more source

Perspectives in educating molecular pathologists on liquid biopsy: Toward integrative, equitable, and decentralized precision oncology

Molecular Oncology, EarlyView.
Liquid biopsy enables minimally invasive, real‐time molecular profiling through analysis of circulating biomarkers in biological fluids. This Perspective highlights the importance of training pathologists through integrative educational programs, such as the European Masters in Molecular Pathology, to ensure effective and equitable implementation of ...
Marius Ilié, Umberto Malapelle, Catherine Alix‐Panabières, Claus Lindbjerg Andersen, Sandra Chlebowski, Vivien Lake, Caroline Lacoux, Virginie Lespinet‐Fabre, Olivier Bordone, Simon Heeke, Christophe Bontoux, Ellen Heitzer, Klaus Pantel, Paul Hofman   +13 more
wiley   +1 more source

Dealing with the problem of non-specific in situ mRNA hybridization signals associated with plant tissues undergoing programmed cell death

Plant Methods, 2010
Background In situ hybridization is a general molecular method typically used for the localization of mRNA transcripts in plants. The method provides a valuable tool to unravel the connection between gene expression and anatomy, especially in species ...
Jokela Anne, Kestilä Johanna, Sääskilahti Mira, Sutela Suvi, Vuosku Jaana, Sarjala Tytti, Häggman Hely   +6 more
doaj   +1 more source

Nucleic acid analysis by sandwich hybridization

Journal of Clinical Laboratory Analysis, 1989
AbstractOne of the most significant achievements of the biochemist during the past two decades is the use to which immunologically based assays have been put in clinical diagnosis (Hood et al.: Immunology, 1984). The problem faced and surmounted by immunologists in effecting the transition from research tool to routine clinical assay bears a remarkable
P J, Nicholls, A D, Malcolm
openaire   +2 more sources

Topological constraints in nucleic acid hybridization kinetics [PDF]

Nucleic Acids Research, 2005
A theoretical examination of kinetic mechanisms for forming knots and links in nucleic acid structures suggests that molecules involving base pairs between loops are likely to become topologically trapped in persistent frustrated states through the mechanism of 'helix-driven wrapping'. Augmentation of the state space to include both secondary structure
Bois, Justin S., Venkataraman, Suvir, Choi, Harry M. T., Spakowitz, Andrew J., Wang, Zhen-Gang, Pierce, Niles A.   +5 more
openaire   +3 more sources