Results 41 to 50 of about 221,973 (265)

Rapid capture of DNA targets

BioTechniques, 2008
A rapid capture technique was developed to efficiently isolate specific DNA targets from a variety of genomes. The specificity can be easily adapted to any target for which partial sequence is known, allowing for the isolation of a wide set of target ...
Judy St. John, Thomas W. Quinn
doaj   +1 more source

Studying epigenetic interactions using MicroScale Thermophoresis (MST)

AIMS Biophysics, 2015
Epigenetic regulation is based on specific molecular interactions between epigenetic reader, writer and eraser molecules and chromatin. Binding parameters of these interactions such as binding affinities, stoichiometries and thermodynamics are essential ...
Thomas Schubert, Gernot Längst
doaj   +1 more source

HiTRACE: High-throughput robust analysis for capillary electrophoresis [PDF]

, 2011
Motivation: Capillary electrophoresis (CE) of nucleic acids is a workhorse technology underlying high-throughput genome analysis and large-scale chemical mapping for nucleic acid structural inference. Despite the wide availability of CE-based instruments,
Bylund, Cormen, Cover, Das, Das, Deigan, Ewing, Ewing, Hanjoo Kim, Jinkyu Kim, Justine Hum, Kay, Kazmi, Kladwang, Kladwang, Laederach, Levenberg, Marquardt, Merino, Mitra, Nielsen, Oppenheim, Peattie, Pravdova, Rhiju Das, Robinson, Ruiz-Martinez, Seunghyun Park, Sungroh Yoon, Tijerina, Tomasi, Vasa, Walczak, Watts, Weeks, Wilkinson, Wipapat Kladwang, Wong, Woolley, Xi   +39 more
core   +1 more source

An efficient algorithm for the stochastic simulation of the hybridization of DNA to microarrays

BMC Bioinformatics, 2009
Background Although oligonucleotide microarray technology is ubiquitous in genomic research, reproducibility and standardization of expression measurements still concern many researchers. Cross-hybridization between microarray probes and non-target ssDNA
Laurenzi Ian J, Arslan Erdem
doaj   +1 more source

On the thermodynamics and kinetics of the cooperative binding of bacteriophage T4-coded gene 32 (helix destabilizing) protein to nucleic acid lattices

Biophysical Journal, 1980
S C Kowalczykowski, Nils Lönberg, John W. Newport, Leland S. Paul, Peter H. von Hippel   +4 more
openalex   +2 more sources

Probing Hybridization parameters from microarray experiments: nearest neighbor model and beyond [PDF]

, 2012
In this article it is shown how optimized and dedicated microarray experiments can be used to study the thermodynamics of DNA hybridization for a large number of different conformations in a highly parallel fashion.
Carlon, Enrico, Hadiwikarta, Wahyu W., Hooyberghs, Jef, Walter, Jean-Charles   +3 more
core   +3 more sources

Direct comparison of nick-joining activity of the nucleic acid ligases from bacteriophage T4 [PDF]

, 2006
The genome of bacteriophage T4 encodes three polynucleotide ligases, which seal the backbone of nucleic acids during infection of host bacteria. The T4Dnl (T4 DNA ligase) and two RNA ligases [T4Rnl1 (T4 RNA ligase 1) and T4Rnl2] join a diverse array of ...
Amitsur, Arabshahi, Bebenek, Begley, Bellacosa, Blondal, Blondal, Cherepanov, Cherepanov, Clepet, Della, Desmond R. Bullard, Doherty, Engler, Fareed, Higgins, Ho, Ho, Kaufmann, Keppetipola, Kornberg, Kuhn, Lavesa-Curto, Lehman, Markham, Martins, Martins, Miller, Moore, Nandakumar, Nandakumar, Nandakumar, Nick McElhinny, Omari, Pascal, Pitcher, Pitcher, Richard P. Bowater, Rossi, Ruiz, Runnels, Rydberg, Sambrook, Sekiguchi, Shuman, Shuman, Snopek, Sriskanda, Stuart, Timson, Tomkinson, Wang, Wang, Weiss, Wilkinson, Wilkinson, Wilkinson, Wood, Wood, Worthey, Yin, Yin   +61 more
core   +5 more sources

Thermodynamic database for protein-nucleic acid interactions

Seibutsu Butsuri, 2000
Protein-nucleic acid interaction plays an essential role in the regulation of gene expression. Structural and binding information provides insight into the mechanism of protein-nucleic acid recognition. Although there are structural databases for protein-nucleic acid complexes, there exists no database for protein-nucleic acid binding.
Ponraj Prabakaran, J. An, M.Michael Gromiha, Samuel Selvaraj, H. Uedaira, H. Kono, A. Sarai   +6 more
openaire   +2 more sources

Improving model predictions for RNA interference activities that use support vector machine regression by combining and filtering features

BMC Bioinformatics, 2007
Background RNA interference (RNAi) is a naturally occurring phenomenon that results in the suppression of a target RNA sequence utilizing a variety of possible methods and pathways.
Peek Andrew S
doaj   +1 more source

Modified RNA triplexes: Thermodynamics, structure and biological potential

Scientific Reports, 2018
The occurrence of triplexes in vivo has been well documented and is determined by the presence of long homopurine-homopyrimidine tracts. The formation of these structures is the result of conformational changes that occur in the duplex, which allow the ...
Marta Szabat, Elzbieta Kierzek, Ryszard Kierzek   +2 more
doaj   +1 more source