Anchoring of Surface Proteins to the Cell Wall of Staphylococcus aureus: sortase catalyzed in vitro transpeptidation reaction using LPXTG peptide and NH2-Gly3 substrates [PDF]
, 2000 Staphylococcus aureus sortase anchors surface proteins to the cell wall envelope by cleaving polypeptides at the LPXTG motif. Surface proteins are linked to the peptidoglycan by an amide bond between the C-terminal carboxyl and the amino group of the ...
Faull, Kym F. +3 more
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Site directed mutagenesis and purification of the cDNA for human class I aldehyde dehydrogenase : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science at Massey University [PDF]
, 1998 Aldehyde dehydrogenase (ALDH) is a key enzyme of alcohol metabolism, removing acetaldehyde which is formed as a product of the alcohol dehydrogenase reaction.
Wansbrough, Erin M
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Bile acids: Lipase-catalyzed synthesis of new hyodeoxycholic acid derivatives [PDF]
, 2018 In this work we present an efficient, environmentally friendly approach to the synthesis of a series of hyodeoxycholic acid derivatives applying Biocatalysis.
Baldessari, Alicia +3 more
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Molecules, 2020 In this study, we present a complete description of the addition of a model nucleophile to the nitroaromatic ring in positions occupied either by hydrogen (the first step of the SNAr-H reaction) or a leaving group (SNAr-X reaction) using theoretical ...
Kacper Błaziak +2 more
doaj +1 more source
A novel Pd-catalysed sequential carbonylation/cyclization approach toward bis-N-heterocycles: rationalization by electronic structure calculations [PDF]
Royal Society Open Science, 2018 An unprecedented palladium-catalysed sequential aminocarbonylation/cyclization synthetic strategy, using carbon monoxide and structurally different aliphatic diamines as N-nucleophiles, gives access, in one pot, to a new family of indole-based N ...
Liliana Damas +6 more
doaj +1 more source
Controllable intein splicing and N-terminal cleavage at mesophilic temperatures
Frontiers in Bioengineering and BiotechnologyInteins (intervening proteins) interrupt host proteins and are removed through a protein splicing reaction that ligates adjacent N- and C-exteins. The ability of inteins to specifically rearrange peptide bonds has proven exceptionally useful in protein ...
Taylor A. McNeal +6 more
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Organic Diselenides: Versatile Reagents, Precursors, and Intriguing Biologically Active Compounds
CHIMIA, 2017 In this account, we describe how some organic diselenides were successfully used in the past as reagents for asymmetric stereoselective synthesis and more recently as precursors of catalysts and reagents applied in new green protocols.
Claudio Santi +2 more
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Nickel-catalyzed transamidation of aliphatic amide derivatives. [PDF]
, 2017 Transamidation, or the conversion of one amide to another, is a long-standing challenge in organic synthesis. Although notable progress has been made in the transamidation of primary amides, the transamidation of secondary amides has remained ...
Baker, Emma L +2 more
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Deactivation of gold(I) catalysts in the presence of thiols and amines – characterisation and catalysis [PDF]
, 2013 Thiols and amines, which are common heteroatom nucleophiles in gold-catalysed reactions, are known to dampen the reactivity of gold catalysts. In this article, the identity and activity of gold(I) catalysts in the presence of thiols and amines is ...
Green, Samantha L J +3 more
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A non-canonical nucleophile unlocks a new mechanistic pathway in a designed enzyme
Nature CommunicationsDirected evolution of computationally designed enzymes has provided new insights into the emergence of sophisticated catalytic sites in proteins. In this regard, we have recently shown that a histidine nucleophile and a flexible arginine can work in ...
Amy E. Hutton +10 more
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