Results 41 to 50 of about 7,536 (222)

Native morphology of influenza virions

Frontiers in Microbiology, 2012
Influenza A virus is an enveloped virus with a segmented, single-strand, negative-sense RNA genome. Its virions show spherical or filamentous shapes of about 100 nm in diameter and occasionally irregular morphology, which exemplifies the pleomorphic ...
Takeshi eNoda
doaj   +1 more source

Controllable self-replicating RNA vaccine delivered intradermally elicits predominantly cellular immunity

iScience, 2023
Summary: Intradermal delivery of self-replicating RNA (srRNA) is a promising vaccine platform. We have developed an srRNA that functions optimally at around 33°C (skin temperature) and is inactivated at or above 37°C (core body temperature) as a safety ...
Tomokazu Amano, Hong Yu, Misa Amano, Erica Leyder, Maria Badiola, Priyanka Ray, Jiyoung Kim, Akihiro C. Ko, Achouak Achour, Nan-ping Weng, Efrat Kochba, Yotam Levin, Minoru S.H. Ko   +12 more
doaj   +1 more source

Quantitative proteomics identified lncRNA HOTAIR regulated nucleoproteins in HeLa cells

, 2021
124-137Hox transcript antisense intergenic RNA (HOTAIR), which was called oncogenic long non-coding RNA (lncRNA) over expressed in the vast majority of cancer types, regulated various biological processes such as cell growth, apoptosis, proliferation ...
Hu, Fan, Chen, Zhuo, Zhang, Tong-cun, Liu, Cheng Lin Zi, Zheng, Peng   +4 more
core  

A universal SARS‐CoV DNA vaccine inducing highly cross‐reactive neutralizing antibodies and T cells

EMBO Molecular Medicine, 2022
New variants in the SARS‐CoV‐2 pandemic are more contagious (Alpha/Delta), evade neutralizing antibodies (Beta), or both (Omicron). This poses a challenge in vaccine development according to WHO.
Sofia Appelberg, Gustaf Ahlén, Jingyi Yan, Negin Nikouyan, Sofie Weber, Olivia Larsson, Urban Höglund, Soo Aleman, Friedemann Weber, Emma Perlhamre, Johanna Apro, Eva‐Karin Gidlund, Ola Tuvesson, Simona Salati, Matteo Cadossi, Hanna Tegel, Sophia Hober, Lars Frelin, Ali Mirazimi, Matti Sällberg   +19 more
doaj   +1 more source

Binding of RNA by the Nucleoproteins of Influenza Viruses A and B

Viruses, 2016
This paper describes a biochemical study for making complexes between the nucleoprotein of influenza viruses A and B (A/NP and B/NP) and small RNAs (polyUC RNAs from 5 to 24 nucleotides (nt)), starting from monomeric proteins.
Alice Labaronne, Christopher Swale, Alexandre Monod, Guy Schoehn, Thibaut Crépin, Rob W. H. Ruigrok   +5 more
doaj   +1 more source

Viral Infection‐Inspired Autonomous Detection of Fusion‐Competent Viruses for Screening and Environmental Surveillance

Advanced Materials, EarlyView.
Inspired by viral entry mechanisms, the FUSION assay enables autonomous detection of respiratory viruses via membrane fusion–triggered CRISPR‐Cas13a activation. VEACON selectively fuses with fusion‐competent viruses, triggering fluorescence within confined vesicles.
Jae Chul Park, Yidam Song, Hyun‐Woo Choi, Jaeuk Sung, Harin Jin, WonSeok Gwak, Kirim Yoo, Soohwang Lee, Jongeon Park, Jeongmin Kim, Hye‐Jun Jo, Jahyun Koo, Youngdo Jeong, Kwan Hyi Lee, Seung‐Jung Kee, Hojun Kim   +15 more
wiley   +1 more source

Nucleoprotein-based nanoscale assembly [PDF]

Proceedings of the National Academy of Sciences, 1997
A system for addressing in the construction of macromolecular assemblies can be based on the biospecificity of DNA (cytosine-5) methyltransferases and the capacity of these enzymes to form abortive covalent complexes at targeted 5-fluorocytosine residues in DNA.
S S, Smith, L, Niu, D J, Baker, J A, Wendel, S E, Kane, D S, Joy   +5 more
openaire   +2 more sources

Nucleoproteins of Negative Strand RNA Viruses; RNA Binding, Oligomerisation and Binding to Polymerase Co-Factor

Viruses, 2010
Commentary on Tawar, R.G.; Duquerroy, S.; Vonrhein, C.; Varela, P.F.; Damier-Piolle, L.; Castagné, N.; MacLellan, K.; Bedouelle, H.; Bricogne, G.; Bhella, D.; Eléouët, J.-F.; Rey, F.A. Crystal structure of a nucleocapsid-like nucleoprotein-RNA complex of
Thibaut Crépin, Rob W. H. Ruigrok
doaj   +1 more source

Quantitative studies of nucleic acids in the cell with microspectrophotometer. II. Nucleic acid and hemoglobin contents in erythroid cells of frog and hen

, 1959
Using the erythroid cells of Rana nigromaculata the hemoglobin synthesis has been studied in the relation of DNA and RNA contents. Results showed that the hemoglobin synthesis starts in the early stage of erythroblast but becomes marked just before the ...
Utsumi, Kozo
core   +1 more source

Time‐Multiplexed Organic Electrochemical Transistor for Saliva‐Based Rapid Detection of Viral Proteins

Advanced Science, EarlyView.
A time‐multiplexed nanobody‐functionalized organic electrochemical transistor enables rapid and sensitive detection of three respiratory viral proteins from saliva. Sequentially addressed multi‐gate electrodes on a shared channel achieve around 1 fm detection within 15 min without sample preprocessing. Clinical validation demonstrates high specificity,
Tianrui Chang, Yuxiang Ren, Shofarul Wustoni, Atheer Alqatari, Adel Hama, Yazhou Wang, Long Chen, Jessica Parrado Agudelo, Luca Salvigni, Keying Guo, Ashraf Dada, Stefan T. Arold, Raik Grünberg, Sahika Inal   +13 more
wiley   +1 more source