Results 1 to 10 of about 115,586 (202)

Strained Conformations of Nucleosides in Active Sites of Nucleoside Phosphorylases [PDF]

Biomolecules, 2020
Nucleoside phosphorylases catalyze the reversible phosphorolysis of nucleosides to heterocyclic bases, giving α-d-ribose-1-phosphate or α-d-2-deoxyribose-1-phosphate. These enzymes are involved in salvage pathways of nucleoside biosynthesis. The level of
Irina A. Il’icheva, Konstantin M. Polyakov, Sergey N. Mikhailov   +2 more
doaj   +3 more sources

Tri-Cyclic Nucleobase Analogs and Their Ribosides as Substrates of Purine-Nucleoside Phosphorylases. II Guanine and Isoguanine Derivatives [PDF]

Molecules, 2019
Etheno-derivatives of guanine, O6-methylguanine, and isoguanine were prepared and purified using standard methods. The title compounds were examined as potential substrates of purine-nucleoside phosphorylases from various sources in the reverse ...
Alicja Stachelska-Wierzchowska, Jacek Wierzchowski, Michał Górka, Agnieszka Bzowska, Beata Wielgus-Kutrowska   +4 more
doaj   +3 more sources

Nucleoside Phosphorylases make N7-xanthosine [PDF]

Nature Communications
Modern, highly evolved nucleoside-processing enzymes are known to exhibit perfect regioselectivity over the glycosylation of purine nucleobases at N9. We herein report an exception to this paradigm.
Sarah Westarp, Felix Brandt, Lena Neumair, Christina Betz, Amin Dagane, Sebastian Kemper, Christoph R. Jacob, Peter Neubauer, Anke Kurreck, Felix Kaspar   +9 more
doaj   +6 more sources

The chemoenzymatic synthesis of clofarabine and related 2′-deoxyfluoroarabinosyl nucleosides: the electronic and stereochemical factors determining substrate recognition by E. coli nucleoside phosphorylases [PDF]

Beilstein Journal of Organic Chemistry, 2014
Two approaches to the synthesis of 2-chloro-9-(2-deoxy-2-fluoro-β-D-arabinofuranosyl)adenine (1, clofarabine) were studied. The first approach consists in the chemical synthesis of 2-deoxy-2-fluoro-α-D-arabinofuranose-1-phosphate (12a, 2FAra-1P) via ...
Ilja V. Fateev, Konstantin V. Antonov, Irina D. Konstantinova, Tatyana I. Muravyova, Frank Seela, Roman S. Esipov, Anatoly I. Miroshnikov, Igor A. Mikhailopulo   +7 more
doaj   +3 more sources

Efficient Biocatalytic Synthesis of Dihalogenated Purine Nucleoside Analogues Applying Thermodynamic Calculations [PDF]

Molecules, 2020
The enzymatic synthesis of nucleoside analogues has been shown to be a sustainable and efficient alternative to chemical synthesis routes. In this study, dihalogenated nucleoside analogues were produced by thermostable nucleoside phosphorylases in ...
Heba Yehia, Sarah Westarp, Viola Röhrs, Felix Kaspar, Robert T. Giessmann, Hendrik F.T. Klare, Katharina Paulick, Peter Neubauer, Jens Kurreck, Anke Wagner   +9 more
doaj   +3 more sources

Synthesis of 5-oxymethyl-1,2,4-triazole-3-carboxamides [PDF]

Тонкие химические технологии, 2022
Objectives. A key step in the synthesis of natural nucleoside analogs is the formation of a glycosidic bond between the carbohydrate fragment and the heterocyclic base. Glycosylation methods differ in terms of regio- and stereoselectivity.
L. E. Grebenkina, A. N. Prutkov, A. V. Matveev, M. V. Chudinov   +3 more
doaj   +3 more sources

Tricyclic Nucleobase Analogs and Their Ribosides as Substrates and Inhibitors of Purine-Nucleoside Phosphorylases III. Aminopurine Derivatives [PDF]

Molecules, 2020
Etheno-derivatives of 2-aminopurine, 2-aminopurine riboside, and 7-deazaadenosine (tubercidine) were prepared and purified using standard methods. 2-Aminopurine reacted with aqueous chloroacetaldehyde to give two products, both exhibiting substrate ...
Alicja Stachelska-Wierzchowska, Jacek Wierzchowski, Michał Górka, Agnieszka Bzowska, Ryszard Stolarski, Beata Wielgus-Kutrowska   +5 more
doaj   +2 more sources

Anion exchange resins in phosphate form as versatile carriers for the reactions catalyzed by nucleoside phosphorylases [PDF]

Beilstein Journal of Organic Chemistry, 2020
In the present work, we suggested anion exchange resins in the phosphate form as a source of phosphate, one of the substrates of the phosphorolysis of uridine, thymidine, and 1-(β-ᴅ-arabinofuranosyl)uracil (Ara-U) catalyzed by recombinant E. coli uridine
Julia N. Artsemyeva, Ekaterina A. Remeeva, Tatiana N. Buravskaya, Irina D. Konstantinova, Roman S. Esipov, Anatoly I. Miroshnikov, Natalia M. Litvinko, Igor A. Mikhailopulo   +7 more
doaj   +2 more sources

Bacterial Purine Nucleoside Phosphorylases from Mesophilic and Thermophilic Sources: Characterization of Their Interaction with Natural Nucleosides and Modified Arabinofuranoside Analogues [PDF]

Biomolecules
The enzymatic synthesis of nucleoside derivatives is an important alternative to multi-step chemical methods traditionally used for this purpose. Despite several undeniable advantages of the enzymatic approach, there are a number of factors limiting its ...
Irina A. Bychek, Anastasia A. Zenchenko, Maria A. Kostromina, Marat M. Khisamov, Pavel N. Solyev, Roman S. Esipov, Sergey N. Mikhailov, Irina V. Varizhuk   +7 more
doaj   +2 more sources

Enzymatic synthesis and phosphorolysis of 4(2)-thioxo- and 6(5)-azapyrimidine nucleosides by E. coli nucleoside phosphorylases [PDF]

Beilstein Journal of Organic Chemistry, 2016
The trans-2-deoxyribosylation of 4-thiouracil (4SUra) and 2-thiouracil (2SUra), as well as 6-azauracil, 6-azathymine and 6-aza-2-thiothymine was studied using dG and E.
Vladimir A. Stepchenko, Anatoly I. Miroshnikov, Frank Seela, Igor A. Mikhailopulo   +3 more
doaj   +2 more sources