Nucleoside Phosphorylases [PDF]
Current Organic Chemistry, 2006 Nucleoside phosphorylases (NPs) are transferases that catalyse the reversible cleavage of the glycosidic bond of ribo- or deoxyribo nucleosides, in the presence of inorganic phosphate, to generate the base and ribose- or deoxyribose- 1-phosphate.
E. Lewkowicz, A. Iribarren
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Croatica Chemica Acta, 2016 It is generaly believed that enzymes retain most of their functionality in the crystal form due to the large solvent content of protein crystals. This is facilitated by the fact that their natural environment in solution is not too far from the one found
Marija Luić, Zoran Štefanić
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Insights into phosphate cooperativity and influence of substrate modifications on binding and catalysis of hexameric purine nucleoside phosphorylases. [PDF]
PLoS ONE, 2012 The hexameric purine nucleoside phosphorylase from Bacillus subtilis (BsPNP233) displays great potential to produce nucleoside analogues in industry and can be exploited in the development of new anti-tumor gene therapies.
Priscila O de Giuseppe +5 more
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Engineering a Bifunctional Fusion Purine/Pyrimidine Nucleoside Phosphorylase for the Production of Nucleoside Analogs [PDF]
BiomoleculesNucleoside phosphorylases (NPs) are pivotal enzymes in the salvage pathway, catalyzing the reversible phosphorolysis of nucleosides to produce nucleobases and α-D-ribose 1-phosphate.
Daniel Hormigo +4 more
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Biosynthesis of Arabinoside from Sucrose and Nucleobase via a Novel Multi-Enzymatic Cascade [PDF]
BiomoleculesArabinoside and derived nucleoside analogs, a family of nucleoside analogs, exhibit diverse typically biological activities and are widely used as antibacterial, antiviral, anti-inflammatory, antitumor, and other drugs in clinical and preclinical trials.
Yuxue Liu +6 more
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Conservation of structure and activity in Plasmodium purine nucleoside phosphorylases. [PDF]
BMC Struct Biol, 2009 BackgroundPurine nucleoside phosphorylase (PNP) is central to purine salvage mechanisms in Plasmodium parasites, the causative agents of malaria. Most human malaria results from infection either by Plasmodium falciparum (Pf), the deadliest form of the ...
Chaikuad A, Brady RL.
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Synthesis of Substituted 1,2,4-Triazole-3-Thione Nucleosides Using E. coli Purine Nucleoside Phosphorylase [PDF]
Biomolecules1,2,4-Triazole derivatives have a wide range of biological activities. The most well-known drug that contains 1,2,4-triazole as part of its structure is the nucleoside analogue ribavirin, an antiviral drug. Finding new nucleosides based on 1,2,4-triazole
Ilya V. Fateev +22 more
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Thermus thermophilus nucleoside phosphorylases active in the synthesis of nucleoside analogues. [PDF]
Appl Environ Microbiol, 2012 Almendros M, Berenguer J, Sinisterra JV.
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The crystal structure of Streptococcus pyogenes uridine phosphorylase reveals a distinct subfamily of nucleoside phosphorylases. [PDF]
Biochemistry, 2011 Tran TH +7 more
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Commonality of Mechanism in Glycoside Hydrolases, Nucleoside Hydrolases, and Phosphorylases: Importance of Side-Chain Conformation Preorganization [PDF]
JACS AuPo-Sen Tseng +3 more
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