Results 1 to 10 of about 458,823 (303)

Rapid single nucleotide polymorphism mapping in C. elegans [PDF]

BMC Genomics, 2005
Background In C. elegans, single nucleotide polymorphisms (SNPs) can function as silent genetic markers, with applications ranging from classical two- and three-factor mapping to measuring recombination across whole chromosomes. Results Here, we describe
Hullett Patrick, Harrach Tracey, Hammarlund Marc, Davis M Wayne, Olsen Shawn, Jorgensen Erik M   +5 more
doaj   +3 more sources

Optimizing the GATA-3 position weight matrix to improve the identification of novel binding sites [PDF]

BMC Genomics, 2012
Background The identifying of binding sites for transcription factors is a key component of gene regulatory network analysis. This is often done using position-weight matrices (PWMs).
Nandi Soumyadeep, Ioshikhes Ilya
doaj   +3 more sources

Nucleotide-resolution mapping of regulatory elements via allelic readout of tiled base editing [PDF]

Nature Communications
CRISPR tiling screens have enabled the characterization of regulatory sequences but are limited by low resolution arising from the indirect readout of editing via guide RNA sequencing and enrichment analysis.
Basheer Becerra, Sandra Wittibschlager, Zain M. Patel, Ana P. Kutschat, Justin Delano, Eric Che, Anzhelika Tauber, Ting Wu, Marlena Starrs, Christina S. Horstmann, Sophie Müller, Madelynn N. Whittaker, Elise Sylvander, Manfred Lehner, Michael I. Love, Benjamin P. Kleinstiver, Martin Jankowiak, Daniel E. Bauer, Davide Seruggia, Luca Pinello   +19 more
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Development of tri-nucleotide microsatellite markers from Crassostrea hongkongensis using enriched genomic libraries and cross-species amplification in two closely related species

Aquaculture Reports, 2021
An enrichment protocol was used to isolate and characterize tri-nucleotide microsatellite markers in Crassostrea hongkongensis, an economically important and extensively cultured oyster species along the southern coastal areas of China.
Haitao Ma, Yuehuan Zhang, Zhiming Xiang, Yang Zhang, Yanping Qin, Ziniu Yu   +5 more
doaj   +1 more source

Autozygosity Mapping by Genome-wide Single Nucleotide Polymorphism Array Identifies a Novel Homozygous HR Mutation in a Consanguineous Family with Universal Hereditary Hair Loss

International Journal of Dermatology and Venerology, 2021
. Objective:. Isolated hereditary hypotrichosis is caused by mutations in as many as 11 different genes. The conventional mutation detection strategy consists of sequencing of individual candidate genes separately, a time consuming and costly approach ...
Sirous Zeinali, Leila Youssefian, Hassan Vahidnezhad, Amir Hossein Saeidian, Soheila Sotoudeh, Hamideh Bagherian, Jouni Uitto   +6 more
doaj   +1 more source

Metagenomic surveillance for bacterial tick-borne pathogens using nanopore adaptive sampling

Scientific Reports, 2023
Technological and computational advancements in the fields of genomics and bioinformatics are providing exciting new opportunities for pathogen discovery and genomic surveillance.
Evan J. Kipp, Laramie L. Lindsey, Benedict Khoo, Christopher Faulk, Jonathan D. Oliver, Peter A. Larsen   +5 more
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Complex Feline Disease Mapping Using a Dense Genotyping Array

Frontiers in Veterinary Science, 2022
The current feline genotyping array of 63 k single nucleotide polymorphisms has proven its utility for mapping within breeds, and its use has led to the identification of variants associated with Mendelian traits in purebred cats.
Isabel Hernandez, Jessica J. Hayward, Jeff A. Brockman, Michelle E. White, Michelle E. White, Lara Mouttham, Elizabeth A. Wilcox, Susan Garrison, Marta G. Castelhano, John P. Loftus, Filipe Espinheira Gomes, Cheryl Balkman, Marjory B. Brooks, Nadine Fiani, Marnin Forman, Tom Kern, Bruce Kornreich, Eric C. Ledbetter, Santiago Peralta, Angela M. Struble, Lisa Caligiuri, Elizabeth Corey, Lin Lin, Julie Jordan, Danny Sack, Adam R. Boyko, Leslie A. Lyons, Rory J. Todhunter   +27 more
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Fast genetic mapping using insertion-deletion polymorphisms in Caenorhabditis elegans

Scientific Reports, 2021
Genetic mapping is used in forward genetics to narrow the list of candidate mutations and genes corresponding to the mutant phenotype of interest. Even with modern advances in biology such as efficient identification of candidate mutations by whole ...
Ho-Yon Hwang, Jiou Wang
doaj   +1 more source

Quantitative PCR approach to SNP detection and linkage mapping in Caenorhabditis elegans

BioTechniques, 2006
I report a method for single nucleotide polymorphism (SNP) detection and linkage mapping in Caenorhabditis elegans using automated oligonucleotide design and fluorescence-based quantitative PCR detection.
Christopher A. Shelton
doaj   +1 more source

Nucleotide Analog Interference Mapping

Methods, 1999
Single-atom substitution experiments provide atomic resolution biochemical information concerning RNA structure and function. Traditionally, these experiments are performed using chimeric RNAs generated by reassembly of full-length RNA from a synthetic substituted oligonucleotide and a truncated RNA transcript.
S P, Ryder, S A, Strobel
openaire   +2 more sources