Results 11 to 20 of about 280,333 (306)

Mapping of Complex Traits by Single-Nucleotide Polymorphisms [PDF]

The American Journal of Human Genetics, 1998
Molecular geneticists are developing the third-generation human genome map with single-nucleotide polymorphisms (SNPs), which can be assayed via chip-based microarrays. One use of these SNP markers is the ability to locate loci that may be responsible for complex traits, via linkage/linkage-disequilibrium analysis.
Zhao, Lue Ping, Aragaki, Corinne, Hsu, Li, Quiaoit, Filemon   +3 more
openaire   +3 more sources

Epitranscriptomic mapping of RNA modifications at single-nucleotide resolution using rhodamine sequencing (Rho-seq)

STAR Protocols, 2022
Summary: The recent development of epitranscriptomics revealed a new fundamental layer of gene expression, but the mapping of most RNA modifications remains technically challenging. Here, we describe our protocol for Rho-Seq, which enables the mapping of
Olivier Finet, Carlo Yague-Sanz, Damien Hermand   +2 more
doaj   +2 more sources

Mapping of psoralen cross-linked nucleotides in RNA [PDF]

Nucleic Acids Research, 1984
A method is described for using the cross-linking reagent 4'-(hydroxy-methyl)-4,5',8-trimethylpsoralen (HMT) to map base paired regions and higher-order structure within RNA molecules. Applying this method to yeast tRNAPhe, we have specifically identified cross-links within the acceptor stem between U6 X U68, in the D-stem between C11 X C25, and in the
E, Garrett-Wheeler, R E, Lockard, A, Kumar   +2 more
openaire   +2 more sources

Quantitative PCR approach to SNP detection and linkage mapping in Caenorhabditis elegans

BioTechniques, 2006
I report a method for single nucleotide polymorphism (SNP) detection and linkage mapping in Caenorhabditis elegans using automated oligonucleotide design and fluorescence-based quantitative PCR detection.
Christopher A. Shelton
doaj   +1 more source

Genetic association mapping via evolution-based clustering of haplotypes. [PDF]

, 2007
Multilocus analysis of single nucleotide polymorphism haplotypes is a promising approach to dissecting the genetic basis of complex diseases. We propose a coalescent-based model for association mapping that potentially increases the power to detect ...
Ioanna Tachmazidou, Maria De Iorio, Claudio J. Verzilli, De Iorio, Maria, De Iorio, M, Claudio J Verzilli, Verzilli, Claudio J, Tachmazidou, I, Tachmazidou, Ioanna, Verzilli, CJ   +9 more
core   +1 more source

Optimizing the GATA-3 position weight matrix to improve the identification of novel binding sites

BMC Genomics, 2012
Background The identifying of binding sites for transcription factors is a key component of gene regulatory network analysis. This is often done using position-weight matrices (PWMs).
Nandi Soumyadeep, Ioshikhes Ilya
doaj   +1 more source

Nucleotide Analog Interference Mapping

Methods, 1999
Single-atom substitution experiments provide atomic resolution biochemical information concerning RNA structure and function. Traditionally, these experiments are performed using chimeric RNAs generated by reassembly of full-length RNA from a synthetic substituted oligonucleotide and a truncated RNA transcript.
S P, Ryder, S A, Strobel
openaire   +2 more sources

Single nucleotide polymorphisms (SNPs) that map to gaps in the human SNP map [PDF]

Nucleic Acids Research, 2003
An international effort is underway to generate a comprehensive haplotype map (HapMap) of the human genome represented by an estimated 300,000 to 1 million 'tag' single nucleotide polymorphisms (SNPs). Our analysis indicates that the current human SNP map is not sufficiently dense to support the HapMap project.
Circe, Tsui, Laura E, Coleman, Jacqulyn L, Griffith, E Andrew, Bennett, Summer G, Goodson, Jason D, Scott, W Stephen, Pittard, Scott E, Devine   +7 more
openaire   +2 more sources

Large scale single nucleotide polymorphism discovery in unsequenced genomes using second generation high throughput sequencing technology: applied to turkey [PDF]

, 2009
Background The development of second generation sequencing methods has enabled large scale DNA variation studies at moderate cost. For the high throughput discovery of single nucleotide polymorphisms (SNPs) in species lacking a sequenced reference genome,
Kerstens Hindrik HD, Veenendaal, A., Johan T den Dunnen, Martien AM Groenen, Bert W Dibbits, Thomas FC Chin-A-Woeng, Dunnen, J.T., den, Groenen, M.A.M., Dibbits, B.W., Kerstens, H.H.D., Chin-A-Woeng, T.F.C., Dibbits Bert W, Hindrik HD Kerstens, Richard PMA Crooijmans, Veenendaal Albertine, den Dunnen Johan T, Chin-A-Woeng Thomas FC, Crooijmans, R.P.M.A., Albertine Veenendaal, Groenen Martien AM, Crooijmans Richard PMA, den Dunnen, J.T.   +21 more
core   +1 more source

TraV: a genome context sensitive transcriptome browser.

PLoS ONE, 2014
Next-generation sequencing (NGS) technologies like Illumina and ABI Solid enable the investigation of transcriptional activities of genomes. While read mapping tools have been continually improved to enable the processing of the increasing number of ...
Sascha Dietrich, Sandra Wiegand, Heiko Liesegang   +2 more
doaj   +1 more source