Results 41 to 50 of about 262,017 (266)

Genome-wide mapping of nucleotide excision repair with XR-seq [PDF]

Nature Protocols, 2018
Nucleotide excision repair is a versatile mechanism to repair a variety of bulky DNA adducts. We developed excision repair sequencing (XR-seq) to study nucleotide excision repair of DNA adducts in humans, mice, Arabidopsis thaliana, yeast and Escherichia coli. In this protocol, the excised oligomers, generated in the nucleotide excision repair reaction,
Jinchuan Hu, Wentao Li, Ogun Adebali, Yanyan Yang, Onur Oztas, Christopher P. Selby, Aziz Sancar   +6 more
openaire   +4 more sources

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

Mapping Conformational Heterogeneity of Mitochondrial Nucleotide Transporter in Uninhibited States [PDF]

Angewandte Chemie, 2015
AbstractOne of the less well understood aspects of membrane transporters is the dynamic coupling between conformational change and substrate transport. NMR approaches are used herein to investigate conformational heterogeneity of the GTP/GDP carrier (GGC) from yeast mitochondria.
Sounier, Rémy, Bellot, Gaëtan, Chou, J. J.   +2 more
openaire   +2 more sources

An upstream open reading frame regulates expression of the mitochondrial protein Slm35 and mitophagy flux

FEBS Letters, EarlyView.
This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva, Ariann E. Mendoza‐Martínez, P. Abril Medina‐Flores, Carlos Campero‐Basaldua, Alexander DeLuna, Soledad Funes   +5 more
wiley   +1 more source

Precise and Cost-Effective Nanopore Sequencing for Post-GWAS Fine-Mapping and Causal Variant Identification

iScience, 2020
Summary: Fine-mapping of interesting loci discovered by genome-wide association study (GWAS) is mandatory to pinpoint causal variants. Traditionally, this fine-mapping is completed through increasing the genotyping density at candidate loci, for which ...
Tarek Magdy, Hui–Hsuan Kuo, Paul W. Burridge   +2 more
doaj   +1 more source

Mapping-by-sequencing using NGS-based 3′-MACE-Seq reveals a new mutant allele of the essential nodulation gene Sym33 (IPD3) in pea (Pisum sativum L.) [PDF]

PeerJ, 2019
Large collections of pea symbiotic mutants were accumulated in the 1990s, but the causal genes for a large portion of the mutations are still not identified due to the complexity of the task.
Aleksandr I. Zhernakov, Oksana Y. Shtark, Olga A. Kulaeva, Jaroslava V. Fedorina, Alexey M. Afonin, Anna B. Kitaeva, Viktor E. Tsyganov, Fabian Afonso-Grunz, Klaus Hoffmeier, Björn Rotter, Peter Winter, Igor A. Tikhonovich, Vladimir A. Zhukov   +12 more
doaj   +2 more sources

A Robust and Rapid Candidate Gene Mapping Pipeline Based on M2 Populations

Frontiers in Plant Science, 2021
The whole-genome sequencing-based bulked segregant analysis (WGS-BSA) has facilitated the mapping candidate causal variations for cloning target plant genes.
Huangkai Zhou, Huangkai Zhou, Kuanqiang Tang, Kuanqiang Tang, Guang Li, Guang Li, Wenqiang Liu, Hui Yu, Xiaohui Yuan, Suxin Yang, Madan K. Bhattacharyya, Xianzhong Feng   +11 more
doaj   +1 more source

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

FEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li, Pedro A. Sánchez‐Murcia, Bernd Nidetzky   +2 more
wiley   +1 more source

Cell wall target fragment discovery using a low‐cost, minimal fragment library

FEBS Letters, EarlyView.
LoCoFrag100 is a fragment library made up of 100 different compounds. Similarity between the fragments is minimized and 10 different fragments are mixed into a single cocktail, which is soaked to protein crystals. These crystals are analysed by X‐ray crystallography, revealing the binding modes of the bound fragment ligands.
Kaizhou Yan, Mathew Stanley, Olawale Raimi, Andrew T. Ferenbach, Helge C Dorfmueller, Daan M. F. van Aalten   +5 more
wiley   +1 more source

DNA analyses and their applications in plant breeding

Czech Journal of Genetics and Plant Breeding, 2002
In recent years, molecular markers have been developed based on the more detailed knowledge of genome structure. Considerable emphasis has been laid on the use of molecular markers in practical breeding and genotype identification.
J. Ovesná, K. Poláková, L. Leišová
doaj   +1 more source