Results 11 to 20 of about 18,919 (201)

Influence of the Intervertebral Disc Microenvironment on Matrix Synthesis and Metabolism in Goat Nucleus Pulposus Cells. [PDF]

JOR Spine
Goat nucleus pulposus cells cultured under clinically relevant oxygen, glucose, pH, and osmolarity revealed pH to have the most influential impact on viability and glycosaminoglycan synthesis. Interactions between factors showed that disc cell behavior is shaped by the microenvironment, highlighting its importance in regenerative therapy design for the
Wilson N, Néill TN, McDonnell J, McDonnell E, Brama PAJ, Buckley CT.   +5 more
europepmc   +2 more sources

Sex Hormones Attenuate Pro-Inflammatory Gene Expression in Nucleus Pulposus and Annulus Fibrosus Cells in a Cell-Type Specific Manner. [PDF]

JOR Spine
Methodological overview of primary bovine NP and AF cell culture. Pro‐ and anti‐inflammatory gene and protein expression analyses were performed after 24 h of pro‐inflammatory stimulus, followed by 3 days of cotreating cells with sex hormones in addition to pro‐inflammatory cytokines.
Hutchinson JL, Leung AE, Séguin CA.
europepmc   +2 more sources

Friedelin Ameliorates Nucleus Pulposus Inflammation by Increasing p65 Autophagic Degradation to Inhibit NF-κB Signalling Pathway. [PDF]

J Cell Mol Med
ABSTRACT Intervertebral disc degeneration (IVDD) is a prevalent disorder associated with chronic inflammation, significantly affecting spinal health and general quality of life. This study examines the anti‐inflammatory properties of Friedelin (FD) and its impact on the NF‐κB signalling pathway in relation to IVDD.
Tu K, Liao D, Chen Z, Wang Z, Zhao K, Zhong H, Wu S, Zhu H, Xu J, Zhou B, Dai X, Wu Q.   +11 more
europepmc   +2 more sources

Sirt1 protects against intervertebral disc degeneration induced by 1,25-dihydroxyvitamin D insufficiency in mice by inhibiting the NF-κB inflammatory pathway

Journal of Orthopaedic Translation, 2023
Background: It has been demonstrated that vitamin D deficiency is associated with an increased risk of patients developing lumbar disc herniation. However, intervertebral disc degeneration caused by active vitamin D deficiency has not been reported. Thus,
Peng Wang, Cuicui Yang, Jinhong Lu, Yongxin Ren, David Goltzman, Dengshun Miao   +5 more
doaj   +1 more source

Osmotic adaptation of nucleus pulposus cells: the role of aquaporin 1, aquaporin 4 and transient receptor potential vanilloid 4 [PDF]

European Cells & Materials, 2021
The microenvironment of the nucleus pulposus is hyperosmotic and fluctuates diurnally due to mechanical loading. Changes in extracellular osmolality result in cell volume alterations, responsiveness to such changes is essential for cellular homeostasis ...
JW Snuggs, RAD Bunning, CL Le Maitre
doaj   +1 more source

Kallistatin inhibits apoptosis and extracellular matrix degradation of human nucleus pulposus cells by inducing SIRT1 expression

陆军军医大学学报, 2022
Objective To investigate the effect of human tissue kallikrein-binding protein, kallistatin (KS) on intervertebral disc nucleus pulposus cells and the possible mechanism.
LU Enhu, LI Qingshu, TAN Yun
doaj   +1 more source

Hypoxia-induced expression of zinc finger protein A20 inhibits apoptosis and attenuates inflammation in nucleus pulposus cells

陆军军医大学学报, 2023
Objective To explore the effect and mechanism of zinc finger protein A20 in the nucleus pulposus cells of intervertebral disc with hypoxic conditions.
GUO Shuaichi, LIU Hao, HAN Zhenghan, LIU Wenjun, LIU Bo   +4 more
doaj   +1 more source

Traditional Chinese medicine promotes the resorption of herniated intervertebral discs by regulating autophagy and apoptosis

Pharmacological Research - Modern Chinese Medicine, 2022
Yiqi Huoxue Decoction (abbreviated as YH in this paper) is a well-accepted traditional Chinese medicine formula used for the treatment of lumbar disc herniation (LDH).
Feng Mao, Xiaoying Ma, Jingyun Chen, Hongwei Li, Zhijia Ma, Feng Dai, Baixue Xu, Jingtao Liu, Hong Jiang, Pengfei Yu   +9 more
doaj   +1 more source

Propionibacterium acnes evades microbicidal phagocytosis by inhibiting the mitochondrial biogenesis of nucleus pulposus cells. [PDF]

FEBS J
P. acnes infection impairs the mitochondrial biogenesis of nucleus pulposus cells (NPCs) through the AMPK/SIRT‐1/PGC‐1α signaling pathway. This results in impaired mitochondria that are unable to generate sufficient ATP, and impaired delivery of mitochondrial reactive oxygen species to carry out the bactericidal process effectively.
Ren L, Li C, Sun J, Zheng Y, Jiao Y, Zheng J, Zhang F, Lin Y, Wu W, Cao P.   +9 more
europepmc   +2 more sources

MiR-27a regulates apoptosis in nucleus pulposus cells by targeting PI3K. [PDF]

PLoS ONE, 2013
The precise role of apoptosis in the pathogenesis of intervertebral disc degeneration (IDD) remains to be elucidated. We analyzed degenerative nucleus pulposus (NP) cells and found that the expression of miR-27a was increased.
Gang Liu, Peng Cao, Huajiang Chen, Wen Yuan, Jianxi Wang, Xianye Tang   +5 more
doaj   +1 more source