Results 131 to 140 of about 3,753,786 (301)
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
A Minimax Fitting Algorithm for Ultra-Precision Aspheric Surfaces [PDF]
Aspheric lenses show significant superiority over traditional spherical ones. The peak-to-valley form deviation is an important criterion for surface qualities of optical lenses.
Zhang, Xiangchao +2 more
core +3 more sources
A new continuous optimization method of standardized cubic permanent magnets for stellarators
Nuclear FusionStellarator configurations can be realized using permanent magnets in combination with tokamak-like planar coils. From the perspective of engineering feasibility and cost-effectiveness, using standardized cubic permanent magnets with a finite number of ...
Xiangyu Zhang +7 more
doaj +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Efficient numerical algorithms for large-scale conic optimization [PDF]
Conic optimization has been widely used in many areas, such as machine learning, image processing and automatic control. It usually relies on iterative algorithms to find the optimum numerically.
Chen, Yuwen
core +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Application of Exact Newton Optimisation to the Maximum Likelihood Ensemble Filter
Tellus: Series A, Dynamic Meteorology and OceanographyThe Newton method is used for optimisation in the maximum likelihood ensemble filter (MLEF) to improve analysis convergence and accuracy. The proposed method is compared against the original method using the conjugate gradient (CG) method preconditioned ...
Takeshi Enomoto, Saori Nakashita
doaj +1 more source
FEBS Open Bio, EarlyView.The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong +3 more
wiley +1 more source