Results 111 to 120 of about 1,628 (157)

Genetic map of an octopine TI-plasmid

Plasmid, 1979
Abstract Several deletion mutants of an octopine TI-plasmid were mapped by digestion with the restriction enzyme Sma I. The T region, as it is defined on the B6-806 plasmid, does not appear to be an essential area for tumour induction on the plasmid of Ach5. The genes for octopine breakdown, plasmid transfer, and the replicator were roughly localized.
B P, Koekman, G, Ooms, P M, Klapwijk, R A, Schilperoort   +3 more
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Octopine and lactate dehydrogenases in mollusc muscles

Comparative Biochemistry and Physiology, 1970
Abstract 1. 1. Octopine dehydrogenase is widely distributed among various orders of marine molluscs, besides the Cephalopoda previously studied. 2. 2. The presence of this enzyme and of lactate dehydrogenase seems to be mutually exclusive. 3. 3. In a few where both enymes are present, octopine dehydrogenase activity is prominent.
F, Regnouf, , Nguyen-van-Thoai
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Partial purification and properties of octopine dehydrogenase and the formation of octopine inAnodonta cygnea L.

Journal of Comparative Physiology ? B, 1975
1. Octopine dehydrogenase was purified 120-fold from adductor muscle of the fresh water bivalveAnodonta cygnea L. by gel filtration on Sephadex G-100 and chromatography on DEAE-Sephadex A 50 and hydroxylapatite. 2. In comparison to the purified enzyme from marine species the final preparation of the octopine dehydrogenase fromAnodonta ...
Gerd G�de, Manfred Grieshaber
openaire   +1 more source

An enzymatic method for the estimation of octopine

Analytical Biochemistry, 1976
Abstract Octopine can be estimated fluorometrically by the reduction of NAD using octopine dehydrogenase. The method is very sensitive and specific for this unusual amino acid. Octopine can be estimated from neutralized perchloric acid extracts without further treatment.
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Comparison of octopine, histopine, lysopine, and octopinic acid synthesizing activities in sunflower crown gall tissues

Biochemical and Biophysical Research Communications, 1977
Abstract Extracts of sunflower crown gall tissues induced by Agrobacterium tumefaciens strain B6 catalyze the synthesis of octopine, histopine, lysopine and octopinic acid. These compounds are not synthesized either in extracts of crown gall tissues induced by strains AT1 and C58 or in extracts of habituated sunflower callus. All four synthetic
E, Hack, J D, Kemp
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Temperature-Determined Enzymatic Functions in Octopine Dehydrogenase

European Journal of Biochemistry, 1975
We investigated the temperature dependence of several functions of octopine dehydrogenase, a monomeric enzyme extracted from the shell fish Pecten maximus L. We found that six enzymatic functions are temperature independent or change only negligibly with temperatue.
P L, Luisi, A, Baici, A, Olomucki, M O, Doublet   +3 more
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Identification of octopine dehydrogenase from Mytilus galloprovincialis

Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 2011
A cDNA encoding the putative octopine dehydrogenase (OcDH) from the mussel Mytilus galloprovincialis was cloned and sequenced. The complete coding region was expressed in the bacteria Escherichia coli and the recombinant protein was purified. The M. galloprovincialis OcDH appears to have the highest affinity for the amino acid substrate L-arginine (88 ...
Sandra, Vázquez-Dorado, Andrés, Sanjuan, Angel S, Comesaña, Alejandro, de Carlos   +3 more
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Further insight on the transferred-DNA of octopine crown gall

Molecular and General Genetics MGG, 1981
Six octopine tumour lines incited by pTiB6S3, pTiAch5 and pTiA6 on tobacco, Arabidopsis and Petunia were studied by the Southern blotting hybridisation technique in order to define accurately the dimensions of the segments of plasmid origin transferred to the tumourous cell and their organisation in the plant genome.
de Beuckeleer, M., Lemmers, M., de Vos, G., Willmitzer, L., van Montagu, M., Schell, J.   +5 more
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The functional organization of the octopine Agrobacterium tumefaciens plasmid pTiB6S3

Plasmid, 1981
We have used the transposon Tn7 to isolate insertion and deletion mutations in the octopine Ti-plasmid pTiB6S3. Mutations that inactivate most of the known Ti phenotypes have been located on the physical map. Most importantly, we have positioned several regions involved in the determination of oncogenicity.
H, De Greve, H, Decraemer, J, Seurinck, M, Van Montagu, J, Schell   +4 more
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Spectrophotometric studies of binary and ternary complexes of octopine dehydrogenase

Biochimica et Biophysica Acta (BBA) - Enzymology, 1970
Abstract 1. 1. The interaction of octopine dehydrogenase of Pecten maximus with coenzymes and substrate analogues was studied by a spectrophotometric difference method. 2. 2. The coupling of the enzyme with coenzymes gives rise to spectral changes in the ultraviolet region similar to those given by a protonation of the adenine ring; in the ...
D B, Pho, A, Olomucki, C, Huc, N V, Thoai   +3 more
openaire   +2 more sources