Results 151 to 160 of about 14,228 (182)

Versatile synthesis of oligodeoxyribonucleotide–oligospermine conjugates

Nature Protocols, 2007
A protocol for the rapid, automated synthesis of oligospermine-oligonucleotide sequences is described. To this end, a protected spermine phosphoramidite derivative was synthesized in six steps from spermine and used as the fifth synthon in an oligonucleotide synthesizer. Parameters were optimized to reach greater than 95% coupling yields.
Voirin, E., Kotera, M., Behr, J.P.
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PEG-Supported Synthesis of Cyclic Oligodeoxyribonucleotides

Nucleosides and Nucleotides, 1993
Abstract Cyclic oligodeoxyribonucleotides have been synthesized in satisfactory yields by an easy procedure using polyethylene glycol (PEG) as soluble supporting polymer. The present method is particularly suitable for a medium to large scale synthesis.
DE NAPOLI, LORENZO, A. MESSERE, MONTESARCHIO, DANIELA, PICCIALLI, GENNARO, C. SANTACROCE, G. M. BONORA   +5 more
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ChemInform Abstract: Oligodeoxyribonucleotide Phosphotriesters

ChemInform, 1994
AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
M. KOZIOLKIEWICZ, A. WILK
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PHOSPHONOACETATE DERIVATIVES OF OLIGODEOXYRIBONUCLEOTIDES

Nucleosides and Nucleotides, 1996
Abstract A deoxyribodinucleotide phosphonoacetate derivative has been prepared, separated into individual diastereomers, and incorporated into oligodeoxyribonucleotides possessing alternating phosphodiester and phosphonoacetate backbone linkages. The hybridization properties and enzymatic stabilities of these oligonucleotides have been studied.
M Jonathan Rudolph, Michael S. Reitman, Eric W MacMillan, Alan F. Cook   +3 more
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Synthesis of Oligodeoxyribonucleotides Containing Oleylamine Moieties

Russian Journal of Bioorganic Chemistry, 2001
A method for directional introduction of oleylamine residues to any position of oligodeoxyribonucleotides during their automated synthesis was developed. The presence of oleylamine residues in 3'- or 5'-terminal nucleotides was shown to have no effect on the thermodynamic stability of DNA duplexes formed by such oligonucleotides and the complementary ...
S Iu, Andreev, S I, Antsypovich, E M, Volkov, E A, Romanova, T, Hianik, T S, Oretskaia   +5 more
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Antisense c-myc Oligodeoxyribonucleotide Cellular Uptake

Pharmaceutical Research, 1992
Antisense oligonucleotides have therapeutic potential as inhibitors of gene expression. However, the mechanism by which an intact oligonucleotide reaches the intracellular site of action is unknown. In this study, we use an oligodeoxyribonucleotide 21-mer complementary to the translation initiation codon of the c-myc protooncogene to study the ...
S, Wu-Pong, T L, Weiss, C A, Hunt
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Enantio-oligodeoxyribonucleotides.

Nucleic acids symposium series, 1991
As a sugar-modified oligonucleotide, a hexamer of 9-(2-deoxy-beta-L-erythro-pentafuranosyl)-9H-purin-6-amine, [L-(dAp)5dA], was synthesized by triester method. The L-hexamer was resistant to bovine spleen phosphodiesterase. UV absorption studies indicated that L-hexamer formed a complex with poly U but not with poly dT at 0 degrees C.
S, Fujimori, K, Shudo, Y, Hashimoto
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Oligodeoxyribonucleotides from pulse-labeled mammalian cells

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1971
When Chinese hamster cells were pulse-labeled with [3H]thymidine, three classes of labeled polymers were isolated by hydroxyapatite chromatography: (1) small oligomers (Fraction I) which do not adsorb to hydroxyapatite in 0.05 M phosphate buffer, (2) single-stranded subunits (Fraction II) which elute at 0.14–0.16 M phosphate, and (3) longer double ...
E K, Schandl, J H, Taylor
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Meso-oligodeoxyribonucleotides.

Nucleic acids symposium series, 1993
Meso-DNAs, containing L-sugar and D-sugar in an alternate sequence as the backbone [Fig. 1, designated as LD-(dN)n], were prepared as a modificated oligonucleotides of natural- and enantio-DNA. The characteristics of meso-DNAs were analyzed, focusing on LD-(dA)n and LD-(dT)n.
O N, Iwanami, K, Shudo, Y, Hashimoto
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A Chemical 5′-Phosphorylation of Oligodeoxyribonucleotides

DNA, 1986
To circumvent the use of T4 polynucleotide kinase and ATP for the 5'-phosphorylation of synthetic oligodeoxyribonucleotides after deprotection and purification, we have developed a new chemical phosphorylation reagent that can be used on automated DNA synthesis instruments.
T, Horn, M S, Urdea
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