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Rational mpox vaccine design: immunogenicity and protective effect of individual and multicomponent proteins in mice. [PDF]
Cheng X +11 more
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Recognition of mixed-sequence double-stranded DNA regions using chimeric Invader/LNA probes.
Everly ME, Emehiser RG, Hrdlicka PJ.
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Improved synthesis of oligodeoxyribonucleotides
Tetrahedron Letters, 1990An improved synthesis of oligodeoxyribonucleotides was achieved with NPE- and NPEOC-protected phosphoramidites leading to easier and time-saving purification and isolation of the gene fragments.
K P, Stengele, W, Pfleiderer
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Red light activated phosphothioate oligodeoxyribonucleotides
Collection Symposium Series, 2008Hairpin-structured phosphorothioate oligodeoxyribonucleotides containing a singlet oxygen-sensitive linker in the loop were prepared. These compounds do not bind complementary nucleic acids in the dark. Upon irradiation with red light in the presence of chlorine e6 the linker within these compounds is cleaved and a single-stranded ...
Alexandru, Rotaru +2 more
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Random mutagenesis using degenerate oligodeoxyribonucleotides
Gene, 1988An efficient method for random mutagenesis was applied to a 75-bp target sequence. The mutational changes in the target region are introduced by the technique of oligodeoxyribonucleotide(oligo)-directed, site-specific mutagenesis using mixtures of degenerate oligos.
P, Hübner, S, Iida, W, Arber
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Versatile synthesis of oligodeoxyribonucleotide–oligospermine conjugates
Nature Protocols, 2007A protocol for the rapid, automated synthesis of oligospermine-oligonucleotide sequences is described. To this end, a protected spermine phosphoramidite derivative was synthesized in six steps from spermine and used as the fifth synthon in an oligonucleotide synthesizer. Parameters were optimized to reach greater than 95% coupling yields.
Voirin, E., Kotera, M., Behr, J.P.
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PEG-Supported Synthesis of Cyclic Oligodeoxyribonucleotides
Nucleosides and Nucleotides, 1993Abstract Cyclic oligodeoxyribonucleotides have been synthesized in satisfactory yields by an easy procedure using polyethylene glycol (PEG) as soluble supporting polymer. The present method is particularly suitable for a medium to large scale synthesis.
DE NAPOLI, LORENZO +5 more
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