Results 151 to 160 of about 11,991 (190)

Rational mpox vaccine design: immunogenicity and protective effect of individual and multicomponent proteins in mice. [PDF]

open access: yesEmerg Microbes Infect
Cheng X   +11 more
europepmc   +1 more source

Improved synthesis of oligodeoxyribonucleotides

Tetrahedron Letters, 1990
An improved synthesis of oligodeoxyribonucleotides was achieved with NPE- and NPEOC-protected phosphoramidites leading to easier and time-saving purification and isolation of the gene fragments.
K P, Stengele, W, Pfleiderer
openaire   +2 more sources

Red light activated phosphothioate oligodeoxyribonucleotides

Collection Symposium Series, 2008
Hairpin-structured phosphorothioate oligodeoxyribonucleotides containing a singlet oxygen-sensitive linker in the loop were prepared. These compounds do not bind complementary nucleic acids in the dark. Upon irradiation with red light in the presence of chlorine e6 the linker within these compounds is cleaved and a single-stranded ...
Alexandru, Rotaru   +2 more
openaire   +2 more sources

Random mutagenesis using degenerate oligodeoxyribonucleotides

Gene, 1988
An efficient method for random mutagenesis was applied to a 75-bp target sequence. The mutational changes in the target region are introduced by the technique of oligodeoxyribonucleotide(oligo)-directed, site-specific mutagenesis using mixtures of degenerate oligos.
P, Hübner, S, Iida, W, Arber
openaire   +2 more sources

Versatile synthesis of oligodeoxyribonucleotide–oligospermine conjugates

Nature Protocols, 2007
A protocol for the rapid, automated synthesis of oligospermine-oligonucleotide sequences is described. To this end, a protected spermine phosphoramidite derivative was synthesized in six steps from spermine and used as the fifth synthon in an oligonucleotide synthesizer. Parameters were optimized to reach greater than 95% coupling yields.
Voirin, E., Kotera, M., Behr, J.P.
openaire   +3 more sources

PEG-Supported Synthesis of Cyclic Oligodeoxyribonucleotides

Nucleosides and Nucleotides, 1993
Abstract Cyclic oligodeoxyribonucleotides have been synthesized in satisfactory yields by an easy procedure using polyethylene glycol (PEG) as soluble supporting polymer. The present method is particularly suitable for a medium to large scale synthesis.
DE NAPOLI, LORENZO   +5 more
openaire   +3 more sources

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