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A microfluidic oligonucleotide synthesizer [PDF]
Nucleic Acids Research, 2010 De novo gene and genome synthesis enables the design of any sequence without the requirement of a pre-existing template as in traditional genetic engineering methods. The ability to mass produce synthetic genes holds great potential for biological research, but widespread availability of de novo DNA constructs is currently hampered by their high cost ...
Lee, Cheng-Chung +2 more
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Making Oligonucleotide Conjugates and Breaking Oligonucleotides [PDF]
Nucleic Acids Symposium Series, 2007 Presented is our most recent work on synthesis and properties of oligonucleotide analogues and conjugates These include 2'-carbamoylmethyl derivatives, peptideoligonucleotide conjugates and conjugates made to combine Watson-Crick and non-Watson-Crick interactions as well as conjugates with RNA-cleaving groups.
Esther Yeheskiely +4 more
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Journal of the American Chemical Society, 2014 Here we report the preparation of poly(oligonucleotide) brush polymers and amphiphilic brush copolymers from nucleic acid monomers via graft-through polymerization. We describe the polymerization of PNA-norbornyl monomers to yield poly-PNA (poly(peptide nucleic acid)) via ring-opening metathesis polymerization (ROMP) with the initiator, (IMesH2)(C5H5N ...
James, Carrie R. +6 more
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Direct oligonucleotide sequencing with nanopores [PDF]
Open Research Europe, 2020 Third-generation DNA sequencing has enabled sequencing of long, unamplified DNA fragments with minimal steps. Direct sequencing of ssDNA or RNA gives valuable insights like base-level modifications, phosphoramidite synthesis yield estimates and strand quality analysis, without the need to add the complimentary strand.
Chalapati, Sachin +3 more
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Oligonucleotides-Based Therapeutics [PDF]
Biomedicines, 2021 This Special Issue of Biomedicines aims to outline nucleic-acid-based strategies that have emerged as tools to regulate specific gene expression and, more recently, as a new class of medicines [...]
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Antisense oligonucleotides [PDF]
Neurology Genetics, 2019 There are few disease-modifying therapeutics for neurodegenerative diseases, but successes on the development of antisense oligonucleotide (ASO) therapeutics for spinal muscular atrophy and Duchenne muscular dystrophy predict a robust future for ASOs in medicine.
Eric Vallabh Minikel +2 more
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Antisense oligonucleotides for neurodegeneration [PDF]
Science, 2020 Promising clinical results for Huntington's disease give hope for other ...
Leavitt, BR, Tabrizi, SJ
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Synthesis of Unmodified Oligonucleotides [PDF]
Current Protocols in Nucleic Acid Chemistry, 2002 Peer Reviewed ; https://deepblue.lib.umich.edu/bitstream/2027.42/152987/1/cpnc0300 ...
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, 2019 Oligonucleotides are short chains of single stranded or double stranded nucleotides, either DNA, RNA or a combination and in some cases chemically modified to improve their application as a drug substance. They are applied to modulate endogenous mechanisms of DNA and RNA processing, thereby modifying disease pathways at the genetic level, rather than ...
Schiffelers, Raymond M. +2 more
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Oligonucleotides as Radiopharmaceuticals
, 2006 Oligonucleotide radiopharmaceuticals is a slowly but steadily progressing field. With a panel of labeling and imaging techniques mastered by several groups, imaging of oligonucleotides is now relatively accessible and will be applied to more and more trials of oligonucleotide pharmacology.
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