Competition between self-assembly and phase separation governs high-temperature condensation of a DNA liquid [PDF]
Physical Review Letters 132, 208401 2024, 2023 In many biopolymer solutions, attractive interactions that stabilize finite-sized clusters at low concentrations also promote phase separation at high concentrations. Here we study a model biopolymer system that exhibits the opposite behavior: Self-assembly of DNA oligonucleotides into finite-sized, stoichiometric clusters, known as "DNA nanostars ...
arxiv +1 more source
Application of chicken microarrays for gene expression analysis in other avian species [PDF]
, 2009 BACKGROUND: With the threat of emerging infectious diseases such as avian influenza, whose natural hosts are thought to be a variety of wild water birds including duck, we are armed with very few genomic resources to investigate large scale immunological
Burggraaf, Simon +3 more
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A model for sequential evolution of ligands by exponential enrichment (SELEX) data [PDF]
Annals of Applied Statistics 2012, Vol. 6, No. 3, 928-949, 2012 A Systematic Evolution of Ligands by EXponential enrichment (SELEX) experiment begins in round one with a random pool of oligonucleotides in equilibrium solution with a target. Over a few rounds, oligonucleotides having a high affinity for the target are selected.
arxiv +1 more source
Physico-chemical foundations underpinning microarray and next-generation sequencing experiments [PDF]
, 2013 Hybridization of nucleic acids on solid surfaces is a key process involved in high-throughput technologies such as microarrays and, in some cases, next-generation sequencing (NGS).
A. Buhot +70 more
core +8 more sources
Investigating synthetic oligonucleotide targeting of miR31 in Duchenne muscular dystrophy [PDF]
, 2016 Exon-skipping via synthetic antisense oligonucleotides represents one of the most promising potential therapies for Duchenne muscular dystrophy (DMD), yet this approach is highly sequence-specific and thus each oligonucleotide is of benefit to only a ...
Hildyard, J C W, Wells, D J
core +1 more source
, 2010 Superparamagnetic iron oxide nanometre scale particles have been utilised as contrast agents to image staked target binding oligonucleotide arrays using MRI to correlate the signal intensity and relaxation times in different NMR ...
Bencsik, Martin +6 more
core +1 more source
Rapid generation of chromosome-specific alphoid DNA probes using the polymerase chain reaction [PDF]
, 1992 Non-isotopic in situ hybridization of chromosome-specific alphoid DNA probes has become a potent tool in the study of numerical aberrations of specific human chromosomes at all stages of the cell cycle. In this paper, we describe approaches for the rapid
A Gnirke +33 more
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Watching the hands of the Arabidopsis biological clock [PDF]
, 2001 Oligonucleotide and cDNA microarrays have been used to analyse the mRNA levels of 8,000 genes in Arabidopsis thaliana throughout the day/night cycle. Genes involved in signal transduction and in various metabolic pathways were found to be coordinately ...
Davis, Seth J. +1 more
core +2 more sources
Detection of minority variants within bovine respiratory syncytial virus populations using oligonucleotide-based microarrays [PDF]
, 2008 Microarray technology, originally developed for highly parallel examination of gene expression is regarded as a potential tool in prognosis and diagnosis.
Baranowski, Eric +4 more
core +1 more source
Selection of prebiotic oligonucleotides by cyclic phase separation [PDF]
arXiv, 2022 The emergence of functional oligonucleotides on early Earth required a molecular selection mechanism to screen for specific sequences with prebiotic functions. Cyclic processes such as daily temperature oscillations were ubiquitous in this environment and could trigger oligonucleotide phase separation. Here, we propose sequence selection based on phase
arxiv