Results 11 to 20 of about 631,998 (390)
A microfluidic oligonucleotide synthesizer [PDF]
Nucleic Acids Research, 2010 De novo gene and genome synthesis enables the design of any sequence without the requirement of a pre-existing template as in traditional genetic engineering methods. The ability to mass produce synthetic genes holds great potential for biological research, but widespread availability of de novo DNA constructs is currently hampered by their high cost ...
Lee, Cheng-Chung +2 more
openaire +11 more sources
Making Oligonucleotide Conjugates and Breaking Oligonucleotides [PDF]
Nucleic Acids Symposium Series, 2007 Presented is our most recent work on synthesis and properties of oligonucleotide analogues and conjugates These include 2'-carbamoylmethyl derivatives, peptideoligonucleotide conjugates and conjugates made to combine Watson-Crick and non-Watson-Crick interactions as well as conjugates with RNA-cleaving groups.
Esther Yeheskiely +4 more
openaire +3 more sources
Microarray-based ultra-high resolution discovery of genomic deletion mutations [PDF]
, 2014 BACKGROUND: Oligonucleotide microarray-based comparative genomic hybridization (CGH) offers an attractive possible route for the rapid and cost-effective genome-wide discovery of deletion mutations.
Baban, D. +8 more
core +5 more sources
Journal of the American Chemical Society, 2014 Here we report the preparation of poly(oligonucleotide) brush polymers and amphiphilic brush copolymers from nucleic acid monomers via graft-through polymerization. We describe the polymerization of PNA-norbornyl monomers to yield poly-PNA (poly(peptide nucleic acid)) via ring-opening metathesis polymerization (ROMP) with the initiator, (IMesH2)(C5H5N ...
James, Carrie R. +6 more
openaire +2 more sources
Enhancing Terminal Deoxynucleotidyl Transferase Activity on Substrates with 3' Terminal Structures for Enzymatic De Novo DNA Synthesis. [PDF]
, 2020 Enzymatic oligonucleotide synthesis methods based on the template-independent polymerase terminal deoxynucleotidyl transferase (TdT) promise to enable the de novo synthesis of long oligonucleotides under mild, aqueous conditions.
Arlow, Daniel H +4 more
core +1 more source
Bioinform., 2003 MOTIVATION When running experiments that involve multiple high density oligonucleotide arrays, it is important to remove sources of variation between arrays of non-biological origin. Normalization is a process for reducing this variation. It is common to
B. Bolstad +3 more
semanticscholar +1 more source
Molecular Therapy: Nucleic Acids, 2013 Lung pathology in cystic fibrosis is linked to dehydration of the airways epithelial surface which in part results from inappropriately raised sodium reabsorption through the epithelial sodium channel (ENaC).
Kenneth L Clark +23 more
doaj +1 more source
Emerging Microbes and Infections, 2021 Candida auris has arisen as an important multidrug-resistant fungus because of several nosocomial outbreaks and elevated rates of mortality. Accurate and rapid diagnosis of C.
Luis Pla +8 more
doaj +1 more source
Proceedings of the National Academy of Sciences of the United States of America, 1999 Oligonucleotide arrays can provide a broad picture of the state of the cell, by monitoring the expression level of thousands of genes at the same time. It is of interest to develop techniques for extracting useful information from the resulting data sets.
U. Alon +6 more
semanticscholar +1 more source
Molecular Therapy: Nucleic Acids, 2012 Small noncoding RNAs (ncRNAs) have been shown to guide epigenetic silencing complexes to target loci in human cells. When targeted to gene promoters, these small RNAs can lead to long-term stable epigenetic silencing of gene transcription. To date, small
Stuart Knowling +6 more
doaj +1 more source