Results 11 to 20 of about 149,559 (187)

Enhanced high density oligonucleotide array-based sequence analysis using modified nucleoside triphosphates [PDF]

Nucleic Acids Research, 1998
Pairs of high density oligonucleotide arrays (DNA chips) consisting of >96 000 oligonucleotides were designed to screen the entire 5.53 kb coding region of the hereditary breast and ovarian cancer BRCA1 gene for all possible sequence changes in the homozygous and heterozygous states.
J. Hacia
semanticscholar   +5 more sources

Rapid p53 sequence analysis in primary lung cancer using an oligonucleotide probe array [PDF]

Proceedings of the National Academy of Sciences, 1999
The p53 gene was sequenced in 100 primary human lung cancers by using direct dideoxynucleotide cycle sequencing and compared with sequence analysis by using the p53 GeneChip assay. Differences in sequence analysis between the two techniques were further evaluated to determine the accuracy and limitations of each method.
Steven A. Ahrendt, Sarel Halachmi, John T. Chow, Wu Li, Naomi Halachmi, Stephen C. Yang, Scott L. Wehage, Jin Jen, David Sidransky   +8 more
semanticscholar   +5 more sources

Optimizing copy number variation analysis using genome-wide short sequence oligonucleotide arrays [PDF]

Nucleic Acids Research, 2010
The detection of copy number variants (CNV) by array-based platforms provides valuable insight into understanding human diversity. However, suboptimal study design and data processing negatively affect CNV assessment. We quantitatively evaluate their impact when short-sequence oligonucleotide arrays are applied (Affymetrix Genome-Wide Human SNP Array 6.
Derek A. Oldridge, Samprit Banerjee, Sunita R. Setlur, Andrea Sboner, Francesca Demichelis   +4 more
semanticscholar   +8 more sources

Experimental optimization of probe length to increase the sequence specificity of high-density oligonucleotide microarrays

BMC Genomics, 2007
Background High-density oligonucleotide arrays are widely used for analysis of genome-wide expression and genetic variation. Affymetrix GeneChips – common high-density oligonucleotide arrays – contain perfect match (PM) and mismatch (MM) probes generated
Kashiwagi Akiko, Furusawa Chikara, Ono Naoaki, Suzuki Shingo, Yomo Tetsuya   +4 more
doaj   +2 more sources

Identification of a deep intronic mutation in the COL6A2 gene by a novel custom oligonucleotide CGH array designed to explore allelic and genetic heterogeneity in collagen VI-related myopathies

BMC Medical Genetics, 2010
Background Molecular characterization of collagen-VI related myopathies currently relies on standard sequencing, which yields a detection rate approximating 75-79% in Ullrich congenital muscular dystrophy (UCMD) and 60-65% in Bethlem myopathy (BM ...
Merlini Luciano, Bertini Enrico, Mercuri Eugenio, Grumati Paolo, Fabris Marina, Sabatelli Patrizia, Urciuolo Anna, Martoni Elena, Neri Marcella, Bovolenta Matteo, Bonaldo Paolo, Ferlini Alessandra, Gualandi Francesca   +12 more
doaj   +2 more sources

Effective primer design for genotype and subtype detection of highly divergent viruses in large scale genome datasets. [PDF]

BMC Bioinformatics
Identification of microorganisms in a biological sample is a crucial step in diagnostics, pathogen screening, biomedical research, evolutionary studies, agriculture, and biological threat assessment.
Demiralay B, Can T.
europepmc   +2 more sources

Sequencing analysis of HPV-other type on an HPV DNA chip [PDF]

Obstetrics & Gynecology Science, 2018
ObjectivesTo identify the specific human papillomavirus (HPV) genotypes from HPV-other type on an HPV DNA chip test by sequencing.MethodsAmong 13,600 women undergoing a routine gynecology examination including Pap smear and/or HPV test by DNA chip test ...
Min-Jeong Kim, Jin Ju Kim, Sunmie Kim
doaj   +1 more source

Identification of genomic imbalances in oral clefts

Jornal de Pediatria, 2021
Objective: This article presents a clinical and cytogenomic approach that focuses on the diagnosis of syndromic oral clefts (OCs). Methods: The inclusion criteria were individuals with OC presenting four or more minor signs and no major defects (non ...
Elaine Lustosa-Mendes, Ana P. dos Santos, Társis P. Vieira, Erlane M. Ribeiro, Adriana A. Rezende, Agnes C. Fett-Conte, Denise P. Cavalcanti, Têmis M. Félix, Isabella L. Monlleó, Vera Lúcia Gil-da-Silva-Lopes   +9 more
doaj   +1 more source

Systematic validation and atomic force microscopy of non-covalent short oligonucleotide barcode microarrays. [PDF]

PLoS ONE, 2008
BACKGROUND: Molecular barcode arrays provide a powerful means to analyze cellular phenotypes in parallel through detection of short (20-60 base) unique sequence tags, or "barcodes", associated with each strain or clone in a collection.
Michael A Cook, Chi-Kin Chan, Paul Jorgensen, Troy Ketela, Daniel So, Mike Tyers, Chi-Yip Ho   +6 more
doaj   +1 more source

GSMA: Gene Set Matrix Analysis, An Automated Method for Rapid Hypothesis Testing of Gene Expression Data [PDF]

Bioinformatics and Biology Insights, 2007
Background Microarray technology has become highly valuable for identifying complex global changes in gene expression patterns. The assignment of functional information to these complex patterns remains a challenging task in effectively interpreting data and correlating results from across experiments, projects and laboratories. Methods which allow the
James P. Hamilton, Yuriko Mori, Takatsugu Kan, Stefan David, Jian Yang, Alexandru Olaru, John M. Abraham, Stephen J. Meltzer, Fumiaki Sato, Zhe Jin, Bogdan C. Paun, Yulan Cheng, Rachana Agarwal, Tetsuo Ito   +13 more
openaire   +35 more sources