Results 41 to 50 of about 127,617 (216)

MicroRNA Expression Profiling by Bead Array Technology in Human Tumor Cell Lines Treated with Interferon-Alpha-2a

Biological Procedures Online, 2009
MicroRNAs are positive and negative regulators of eukaryotic gene expression that modulate transcript abundance by specific binding to sequence motifs located prevalently in the 3' untranslated regions of target messenger RNAs (mRNA). Interferon-alpha-2a
Siegrist Fredy, Singer Thomas, Certa Ulrich   +2 more
doaj   +3 more sources

Strong position-dependent effects of sequence mismatches on signal ratios measured using long oligonucleotide microarrays

BMC Genomics, 2008
Background Microarrays are an important and widely used tool. Applications include capturing genomic DNA for high-throughput sequencing in addition to the traditional monitoring of gene expression and identifying DNA copy number variations.
Hulme Helen, Kemp Stephen J, Noyes Harry A, Rennie Catriona, Brass Andy, Hoyle David C   +5 more
doaj   +1 more source

Motif effects in Affymetrix GeneChips seriously affect probe intensities [PDF]

, 2012
An Affymetrix GeneChip consists of an array of hundreds of thousands of probes (each a sequence of 25 bases) with the probe values being used to infer the extent to which genes are expressed in the biological material under investigation. In this article,
Andrew P. Harrison, Arteaga-Salas, Arteaga-Salas, Barrett, Binder, Binder, Burden, Do, Fasold, Friedman, Geller, Gharaibeh, Graham J. G. Upton, Irizarry, Irizarry, Kerkhoven, Langdon, McCall, Mei, Memon, Memon, Mulders, Naef, Shanahan, Upton, Upton, Upton, Wanke   +27 more
core   +1 more source

Tumor‐stromal crosstalk and macrophage enrichment are associated with chemotherapy response in bladder cancer

FEBS Open Bio, EarlyView.
Chemoresistance in bladder cancer: Macrophage recruitment associated with CXCL1, CXCL5 and CXCL8 expression is characteristic of Gemcitabine/Cisplatin (Gem/Cis) Non‐Responder tumors (right side) while Responder tumors did not show substantial tumor‐stromal crosstalk (left side). All biological icons are attributed to Bioicons: carcinoma, cancerous‐cell‐
Sophie Leypold, Janik Riese, Lancelot Seillier, Mark Kühnel, Julia Pannhausen, Charlotte O. J. Fröhlich, Christian Martin, Peter Boor, Matthias Saar, Danny D. Jonigk, Nadine T. Gaisa, Michael Rose   +11 more
wiley   +1 more source

High-Throughput Methods for Detection of Genetic Variation

BioTechniques, 2001
Understanding human genetic variation is currently believed to reveal the cause of individual susceptibility to disease and the large variation observed in response to treatment.
Vessela Nedelcheva Kristensen, Dimitris Kelefiotis, Tom Kristensen, Anne-Lise Børresen-Dale   +3 more
doaj   +1 more source

Locked nucleic acid: tighter is different [PDF]

, 2013
This viewpoint briefly reviews the impact of Locked Nucleic Acid (LNA) oligonucleotides, first described in a ChemComm paper in 1998. A number of unique applications in oligonucleotide biotechnology have been made possible by the high binding affinity ...
Watts, Jonathan
core   +1 more source

Differential regulation of ZFAS1 splice variants by endoplasmic reticulum stress in hepatocyte cell lines

FEBS Open Bio, EarlyView.
ZFAS1 is a lncRNA promoting cell proliferation and migration, exhibiting high expression in various cancers. It is conserved, widely expressed, and produces multiple splice variants with unclear roles. We identified several splice variants in hepatocyte models, and found that inhibiting or suppressing regulators of the unfolded protein response (PERK ...
Sébastien Soubeyrand, Paulina Lau, Ruth McPherson   +2 more
wiley   +1 more source

High-throughput Microarray Detection of Vomeronasal Receptor Gene Expression in Rodents

Frontiers in Neuroscience, 2010
We performed comprehensive data mining to explore the vomeronasal receptor (V1R & V2R) repertoires in mouse and rat using the mm5 and rn3 genome, respectively.
Xiaohong Zhang, Florencia Marcucci, Stuart Firestein   +2 more
doaj   +1 more source

Affymetrix probes containing runs of contiguous guanines are not gene-specific [PDF]

, 2008
High Density Oligonucleotide arrays (HDONAs), such as the Affymetrix HG-U133A GeneChip, use sets of probes chosen to match specified genes, with the expectation that if a particular gene is highly expressed then all the probes in the designated probe set
Andrew P. Harrison, Graham J. Upton, William B. Langdon   +2 more
core   +1 more source

Digital twins to accelerate target identification and drug development for immune‐mediated disorders

FEBS Open Bio, EarlyView.
Digital twins integrate patient‐derived molecular and clinical data into personalised computational models that simulate disease mechanisms. They enable rapid identification and validation of therapeutic targets, prediction of drug responses, and prioritisation of candidate interventions.
Anna Niarakis, Philippe Moingeon
wiley   +1 more source