Results 51 to 60 of about 129,877 (153)

Motif effects in Affymetrix GeneChips seriously affect probe intensities [PDF]

, 2012
An Affymetrix GeneChip consists of an array of hundreds of thousands of probes (each a sequence of 25 bases) with the probe values being used to infer the extent to which genes are expressed in the biological material under investigation. In this article,
Andrew P. Harrison, Arteaga-Salas, Arteaga-Salas, Barrett, Binder, Binder, Burden, Do, Fasold, Friedman, Geller, Gharaibeh, Graham J. G. Upton, Irizarry, Irizarry, Kerkhoven, Langdon, McCall, Mei, Memon, Memon, Mulders, Naef, Shanahan, Upton, Upton, Upton, Wanke   +27 more
core   +1 more source

A simple optimization can improve the performance of single feature polymorphism detection by Affymetrix expression arrays

BMC Genomics, 2010
Background High-density oligonucleotide arrays are effective tools for genotyping numerous loci simultaneously. In small genome species (genome size: < ~300 Mb), whole-genome DNA hybridization to expression arrays has been used for various applications ...
Fujisawa Hironori, Harushima Yoshiaki, Horiuchi Youko, Mochizuki Takako, Kawakita Masanori, Sakaguchi Takayuki, Kurata Nori   +6 more
doaj   +1 more source

Affymetrix probes containing runs of contiguous guanines are not gene-specific [PDF]

, 2008
High Density Oligonucleotide arrays (HDONAs), such as the Affymetrix HG-U133A GeneChip, use sets of probes chosen to match specified genes, with the expectation that if a particular gene is highly expressed then all the probes in the designated probe set
Andrew P. Harrison, Graham J. Upton, William B. Langdon   +2 more
core   +1 more source

An ultrasensitive photoelectrochemical nucleic acid biosensor [PDF]

, 2005
A simple and ultrasensitive procedure for non-labeling detection of nucleic acids is described in this study. It is based on the photoelectrochemical detection of target nucleic acids by forming a nucleic acid/photoreporter adduct layer on an ITO ...
Gao, Zhiqiang, Tansil, Natalia C.
core   +3 more sources

Alternative mapping of probes to genes for Affymetrix chips

BMC Bioinformatics, 2004
Background Short oligonucleotide arrays have several probes measuring the expression level of each target transcript. Therefore the selection of probes is a key component for the quality of measurements.
Friis-Hansen Lennart, Møller Morten, Gautier Laurent, Knudsen Steen   +3 more
doaj   +1 more source

Combinatorial CRISPR-Cas9 screens for de novo mapping of genetic interactions. [PDF]

, 2017
We developed a systematic approach to map human genetic networks by combinatorial CRISPR-Cas9 perturbations coupled to robust analysis of growth kinetics.
A Baryshnikova, A Baryshnikova, A DeLean, Aaron N Chang, AE Briner, Alex N Beckett, Alex Thomas, AM Kabadi, Amanda Birmingham, Ana Bojorquez-Gomez, ASL Wong, Assen Roguev, B Vogelstein, C Laufer, Chih-Chung Kuo, CJ Lord, Dan Du, Daniel Pekin, Dongxin Zhao, F Lori, H Xu, J Shi, Jason F Kreisberg, JD Storey, Jens Luebeck, JG Doench, John Paul Shen, Katherine Licon, Kristin Klepper, KS Pollard, Kyle Salinas Sanchez, L Avery, LA Gilbert, Lei Qi, M Costanzo, M Martin, MC Bassik, MS Neshat, Nathan E Lewis, NE Sanjana, Nevan Krogan, NJ Krogan, P Mali, P Mali, Prashant Mali, R Chari, R Mani, R Srivas, Roman Sasik, S Bandyopadhyay, SR Collins, T Hart, T Horn, Trey Ideker, VG Tusher, Y Benjamini, Y Dang   +56 more
core   +1 more source

Improved detection of global copy number variation using high density, non-polymorphic oligonucleotide probes

BMC Genetics, 2008
Background DNA sequence diversity within the human genome may be more greatly affected by copy number variations (CNVs) than single nucleotide polymorphisms (SNPs).
Liu Guoying, Zhang Jane, Chen Wenwei, Kirby Andrew, Truong Vivi B, Fitch Karen R, Huang Jing, Shen Fan, McCarroll Steven A, Jones Keith W, Shapero Michael H   +10 more
doaj   +1 more source

Site Selective Antibody-Oligonucleotide Conjugation via Microbial Transglutaminase. [PDF]

, 2019
Nucleic Acid Therapeutics (NATs), including siRNAs and AntiSense Oligonucleotides (ASOs), have great potential to drug the undruggable genome. Targeting siRNAs and ASOs to specific cell types of interest has driven dramatic improvement in efficacy and ...
Cui, Xianshu, Dowdy, Steven F, Huggins, Ian J, Jadhav, Satish, Medina, Carlos A, Springer, Aaron D, van den Berg, Arjen   +6 more
core   +1 more source

In vivo transcriptional profiling of Plasmodium falciparum

Malaria Journal, 2004
Background Both host and pathogen factors contribute to disease outcome in Plasmodium falciparum infection. The feasibility of studying the P. falciparum in vivo transcriptome to understand parasite transcriptional response while it resides in the human ...
Sultan Ali, Mboup Soulyemane, Ndir Omar, Zhou Yingyao, Fang Xuemin, Sarr Ousmane, Le Roch Karine G, Daily Johanna P, Winzeler Elizabeth A, Wirth Dyann F   +9 more
doaj   +1 more source

Identification of a deep intronic mutation in the COL6A2 gene by a novel custom oligonucleotide CGH array designed to explore allelic and genetic heterogeneity in collagen VI-related myopathies

BMC Medical Genetics, 2010
Background Molecular characterization of collagen-VI related myopathies currently relies on standard sequencing, which yields a detection rate approximating 75-79% in Ullrich congenital muscular dystrophy (UCMD) and 60-65% in Bethlem myopathy (BM ...
Merlini Luciano, Bertini Enrico, Mercuri Eugenio, Grumati Paolo, Fabris Marina, Sabatelli Patrizia, Urciuolo Anna, Martoni Elena, Neri Marcella, Bovolenta Matteo, Bonaldo Paolo, Ferlini Alessandra, Gualandi Francesca   +12 more
doaj   +1 more source