Results 51 to 60 of about 708,274 (274)

DNA hybridization to mismatched templates: a chip study [PDF]

, 2001
High-density oligonucleotide arrays are among the most rapidly expanding technologies in biology today. In the {\sl GeneChip} system, the reconstruction of the target concentration depends upon the differential signal generated from hybridizing the target RNA to two nearly identical templates: a perfect match (PM) and a single mismatch (MM) probe.
arxiv   +1 more source

ReseqChip: Automated integration of multiple local context probe data from the MitoChip array in mitochondrial DNA sequence assembly

BMC Bioinformatics, 2009
Background The Affymetrix MitoChip v2.0 is an oligonucleotide tiling array for the resequencing of the human mitochondrial (mt) genome. For each of 16,569 nucleotide positions of the mt genome it holds two sets of four 25-mer probes each that match the ...
Spang Rainer, Parson Walther, Niederstätter Harald, Lottaz Claudio, Thieme Marian, Oefner Peter J   +5 more
doaj   +1 more source

Genomic Analysis Using High-Density Single Nucleotide Polymorphism-Based Oligonucleotide Arrays and Multiplex Ligation-Dependent Probe Amplification Provides a Comprehensive Analysis of INI1/SMARCB1 in Malignant Rhabdoid Tumors

Clinical Cancer Research, 2009
Purpose: A high-resolution genomic profiling and comprehensive targeted analysis of INI1/SMARCB1 of a large series of pediatric rhabdoid tumors was done.
E. Jackson, Angela J. Sievert, X. Gai, H. Hakonarson, A. Judkins, Laura S Tooke, J. Perin, H. Xie, T. Shaikh, J. Biegel   +9 more
semanticscholar   +1 more source

Ubiquitination of transcription factors in cancer: unveiling therapeutic potential

Molecular Oncology, EarlyView.
In cancer, dysregulated ubiquitination of transcription factors contributes to the uncontrolled growth and survival characteristics of tumors. Tumor suppressors are degraded by aberrant ubiquitination, or oncogenic transcription factors gain stability through ubiquitination, thereby promoting tumorigenesis.
Dongha Kim, Hye Jin Nam, Sung Hee Baek
wiley   +1 more source

The basics of commonly used molecular techniques for diagnosis, and application of molecular testing in cytology

Diagnostic Cytopathology, Volume 51, Issue 1, Page 83-94, January 2023., 2023
Abstract Molecular diagnostics has expanded to become the standard of care for a variety of solid tumor types. With limited diagnostic material, it is often desirable to use cytological preparations to provide rapid and accurate molecular results. This review covers important pre‐analytic considerations and limitations, and a description of common ...
Scott A. Turner, Rand Abou Shaar, Zhongbo Yang   +2 more
wiley   +1 more source

Comment on: ``Solving the riddle of the bright mismatches: Labeling and effective binding in oligonucleotide arrays" [PDF]

Phys. Rev. E 73, 063901 (2006), 2006
In a recent paper [Phys. Rev. E 68, 011906 (2003)], Naef and Magnasco suggested that the "bright" mismatches observed in Affymetrix microarray experiments are caused by the fluorescent molecules used to label RNA target sequences, which would impede target-probe hybridization.
arxiv   +1 more source

A high-resolution, nucleosome position map of C. elegans reveals a lack of universal sequence-dictated positioning.

Genome Research, 2008
Using the massively parallel technique of sequencing by oligonucleotide ligation and detection (SOLiD; Applied Biosystems), we have assessed the in vivo positions of more than 44 million putative nucleosome cores in the multicellular genetic model ...
Anton Valouev, J. Ichikawa, T. TonThat, Jeremy R Stuart, S. Ranade, H. Peckham, K. Zeng, J. Malek, G. Costa, K. McKernan, A. Sidow, A. Fire, Steven M. Johnson   +12 more
semanticscholar   +1 more source

ALS plasma biomarkers reveal neurofilament and pTau correlate with disease onset and progression

Annals of Clinical and Translational Neurology, EarlyView.
Abstract Objective We performed a pilot screen to assess the utility of the NULISA™ (Nucleic‐acid‐Linked Immuno‐Sandwich Assay) platform in the identification of amyotrophic lateral sclerosis (ALS) biomarkers. Methods Plasma from 86 individuals (48 ALS, 18 asymptomatic C9orf72 repeat expansion carriers (AsymC9), and 20 healthy controls) was analyzed ...
Eleanor V. Thomas, Changee Han, Woo Jae Kim, Seneshaw Asress, Yingjie Li, Jennifer A. Taylor, Marla Gearing, Christina N. Fournier, Zachary T. McEachin, Nicholas T. Seyfried, Jonathan D. Glass   +10 more
wiley   +1 more source

Selection of prebiotic oligonucleotides by cyclic phase separation [PDF]

arXiv, 2022
The emergence of functional oligonucleotides on early Earth required a molecular selection mechanism to screen for specific sequences with prebiotic functions. Cyclic processes such as daily temperature oscillations were ubiquitous in this environment and could trigger oligonucleotide phase separation. Here, we propose sequence selection based on phase
arxiv  

Encrypted Oligonucleotide Arrays for Molecular Authentication.

ACS Combinatorial Science, 2019
Counterfeiting is an incredibly widespread problem, with some estimates placing its economic impact above 2% of worldwide GDP. The scale of the issue suggests that current preventive measures are either technologically insufficient or too impractical and
Matthew T. Holden, Lloyd M. Smith
semanticscholar   +1 more source