Results 31 to 40 of about 1,424,575 (357)
Development, characterization and experimental validation of a cultivated sunflower (Helianthus annuus L.) gene expression oligonucleotide microarray. [PDF]
PLoS ONE, 2012 Oligonucleotide-based microarrays with accurate gene coverage represent a key strategy for transcriptional studies in orphan species such as sunflower, H. annuus L., which lacks full genome sequences.
Paula Fernandez +17 more
doaj +1 more source
ProbeMaker: an extensible framework for design of sets of oligonucleotide probes
BMC Bioinformatics, 2005 Background Procedures for genetic analyses based on oligonucleotide probes are powerful tools that can allow highly parallel investigations of genetic material.
Nilsson Mats +2 more
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BMC Bioinformatics, 2010 Background Current commercial high-density oligonucleotide microarrays can hold millions of probe spots on a single microscopic glass slide and are ideal for studying the transcriptome of microbial genomes using a tiling probe design.
Chen Tsute, Høvik Hedda
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Generation of Amplifiable Genome-Specific Oligonucleotide Probes and Libraries
BioTechniques, 2002 Here we describe a process for the generation of oligonucleotide libraries representative of a given nucleic acid. Starting from a random pool of DNA oligonucleotides, the technique selects only those that hybridize to the nucleic acid template.
Ivan Brukner +2 more
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Chromosome Research, 2011 The ability to visualize specific DNA sequences, on chromosomes and in nuclei, by fluorescence in situ hybridization (FISH) is fundamental to many aspects of genetics, genomics and cell biology. Probe selection is currently limited by the availability of
S. Boyle +4 more
semanticscholar +1 more source
Oligonucleotide microarray analysis of aminoallyl-labeled cDNA targets from linear RNA amplification
BioTechniques, 2004 Single-stranded long oligonucleotide-based (50- to 70-mer) microarrays offer several advantages over conventional cDNA microarrays. These include the easy preparation of the probes, low cost of array production, and low cross-contamination during probe ...
Pal Kaposi-Novak +4 more
doaj +1 more source
Microarray-Based Method for Combinatorial Library Sequence Mapping and Characterization
BioTechniques, 2003 Here we describe a DNA-chip-based method for high-throughput sequence mapping. This involves competitive hybridization between short and differentially labeled fluorescent oligonucleotide probes and glass-supported PCR products.
V. Abécassis +6 more
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Highly Sensitive and Multiplexed In Situ RNA Profiling with Cleavable Fluorescent Tyramide
Cells, 2021 Understanding the composition, regulation, and function of complex biological systems requires tools that quantify multiple transcripts at their native cellular locations.
Lu Xiao, Joshua Labaer, Jia Guo
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Molecular Oncology, EarlyView.Chronic TGF‐β exposure drives epithelial HCC cells from a senescent state to a TGF‐β resistant mesenchymal phenotype. This transition is characterized by the loss of Smad3‐mediated signaling, escape from senescence, enhanced invasiveness and metastatic potential, and upregulation of key resistance modulators such as MARK1 and GRM8, ultimately promoting
Minenur Kalyoncu +11 more
wiley +1 more source
Molecular probe technology detects bacteria without culture
BMC Microbiology, 2012 Background Our ultimate goal is to detect the entire human microbiome, in health and in disease, in a single reaction tube, and employing only commercially available reagents.
Hyman Richard W +9 more
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