Results 191 to 200 of about 92,911 (310)

Realizing the promise of ‘La Dolce Vita’ via chemical biology: glycan‐motif editing of sLeX for precision cancer therapeutics

open access: yesThe FEBS Journal, EarlyView.
Modification of the glycocalyx via ‘glycan editing’ is distinguishable from ‘glycan‐motif editing’. The former broadly remodels the glycocalyx, whereas the latter inhibits expression only of a specific oligosaccharide motif within the glycocalyx by selectively dampening the bioactivity of the key glycosyltransferase(s) programming the biosynthesis of ...
Barbara Richichi, Robert Sackstein
wiley   +1 more source

Structural and functional insights into extreme thermal stability and activity of two GH12 domains of a multidomain glycosidase from a hyperthermophilic euryarchaeon

open access: yesThe FEBS Journal, EarlyView.
The multidomain glycosidase MDG from the hyperthermophilic euryarchaeon Thermococcus sp. contains five protein domains: three catalytic domains (GH5, GH12‐1, and GH12‐2) and two cellulose‐binding modules (CBM2). In this study, the GH12‐1 and GH12‐2 domains were individually purified and biochemically characterized.
Kseniya S. Zayulina   +11 more
wiley   +1 more source

Electrostatic potential as a reactivity scoring function in computer‐assisted enzyme engineering

open access: yesThe FEBS Journal, EarlyView.
We present a computational metric that quantifies the electrostatic complementarity of enzymes to the charge distribution of substrates at the transition state. Implemented in BindScan, this metric accurately predicts functionally relevant mutations in glycoside hydrolases.
Aitor Vega, Antoni Planas, Xevi Biarnés
wiley   +1 more source

Anions and citrate inhibit LsAA9A, a lytic polysaccharide monooxygenase (LPMO)

open access: yesThe FEBS Journal, EarlyView.
The fungal lytic polysaccharide monooxygenase LsAA9A binds chloride in its crystallization mother liquor, leading to LsAA9A inhibition. Other halides except for fluoride inhibit LsAA9A activity at high concentrations. Transferring crystals to a different solution with lower ionic strength leads to binding of citrate buffer, leading also to the ...
Valerio Di Domenico   +6 more
wiley   +1 more source

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