Results 251 to 260 of about 79,883 (298)

Activation of oocytes by latrunculin A

The FASEB Journal, 2002
ABSTRACT Actin depolymerization by latrunculin A (LAT‐A) in mature starfish oocytes induces a massive calcium mobilization that results in the discharge of the cortical granules and in the elevation of the fertilization envelope.
LIM, DMITRY, Lange, Klaus, Santella, Luigia   +2 more
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Oocyte activation: lessons from human infertility

Trends in Molecular Medicine, 2001
During fertilization, the spermatozoon penetrates through the cumulus cells and the zona pellucida that surrounds the oocyte, before it binds and fuses with the oocyte plasma membrane to induce activation. In vitro fertilization (IVF) studies performed in non-human mammals have contributed extensive knowledge regarding the mechanisms by which the ...
D, Ben-Yosef, R, Shalgi
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Sperm-activated currents in ascidian oocytes

Developmental Biology, 1987
Using patch electrodes and the whole-cell recording technique to study fertilization currents in ascidian oocytes under voltage clamp, this paper shows that between -85 and 0 mV the currents are inward with an initial peak ranging from 50 to 600 pA. Voltages more positive than 0 mV inhibit initiation of the fertilization current, but by allowing the ...
L J, DeFelice, M J, Kell
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Parthenogenetic Activation of Oocytes

Cold Spring Harbor Protocols, 2018
Numerous studies have been initiated to investigate the influence of maternal and paternal genomes on early mammalian development. For this type of study, parthenogenetic embryos provide a unique source of preimplantation and early postimplantation embryos that (by definition) develop in the absence of any contribution from a male gamete ...
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Acetylcholinesterase activity of Xenopus laevis oocytes

Neuroscience, 1983
The cholinesterase activity of Xenopus laevis oocytes was assessed using [3H]acetylcholine in a simple radiometric procedure. The cholinesterase activity of mature (stage V-Vl) oocytes was very sensitive to inhibition by the specific acetylcholinesterase inhibitor, BW284-C5l, and relatively insensitive to an inhibitor of non-specific, or ...
C B, Gundersen, R, Miledi
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Mammalian Oocytes: X Chromosome Activity

Science, 1969
The glucose-6-phosphate dehydrogenase and lactate dehydrogenase contents of oocytes from XO and XX female mice have been measured. The activity of the former in the oocytes of XO mice is half of that in the oocytes of XX mice, whereas the lactate dehydrogenase activities in the two groups of ova are the same.
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Immunocytochemical studies of hamster oocyte activation

Molecular Reproduction and Development, 1990
AbstractBy indirect immunofluorescence, using rabbit anti‐heparin‐binding placental protein (HBPP) antiserum, we studied HBPP expression by physiologically and non‐physiologically (microsurgically) activated hamster gametes. Whereas mature gametes (sperm, metaphase II oocytes) were negative, in vivo conceived preimplantation embryos, from pronuclear to
M J, Sinosich, S E, Lanzendorf, G D, Hodgen   +2 more
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Strategies for activating nuclear transfer oocytes

Reproduction, Fertility and Development, 1999
The technique of nuclear transfer can have enormous applications in the fields of agriculture and biomedicine. This is especially true if a cell line that has been transformed can be used as a source of nuclei for the nuclear transfer. One major aspect of the nuclear transfer procedures is that of oocyte activation.
Z, Macháty, R S, Prather
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Oocyte Activation and Fertilisation: Crucial Contributors from the Sperm and Oocyte

2017
This chapter intends to summarise the importance of sperm- and oocyte-derived factors in the processes of sperm-oocyte binding and oocyte activation. First, we describe the initial interaction between sperm and the zona pellucida, with particular regard to acrosome exocytosis. We then describe how sperm and oocyte membranes fuse, with special reference
Yeste, M, Jones, C, Amdani, S, Coward, K
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Sperm-activated channels in ascidian oocytes

Developmental Biology, 1984
Ionic channels activated during fertilization in the ascidian oocyte membrane have been studied using the single-channel recording technique. These channels, which we have called "fertilization channels," have a single-channel conductance of about 400 pS, a reversal potential of about O mV, and an open time of 2-30 msec. The open channel probability is
B, Dale, L, De Felice
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