Results 91 to 100 of about 47,560,035 (275)

Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding

FEBS Letters, EarlyView.
In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança, Eline L. Jansen, Jorine M. Eeftens   +2 more
wiley   +1 more source

Linked Open Data

, 2020
This book intends to provide the reader with a comprehensive knowledge of the latest developments within the Linked Open Data (LOD) framework and the benefits of supported systems.

core   +1 more source

Combining numbers and perspectives: Using a Scale, Choice and Insight (SCI) approach for online survey research

Methodological Innovations
There is limited methodological guidance on systematically integrating numerical-style responses with open-ended questions in online surveys. This paper addresses this gap by presenting the Scale, Choice and Insight (SCI) approach, which applies to both ...
David Meechan
doaj   +1 more source

Towards linked open gene mutations data

BMC Bioinformatics, 2012
Background With the advent of high-throughput technologies, a great wealth of variation data is being produced. Such information may constitute the basis for correlation analyses between genotypes and phenotypes and, in the future, for personalized ...
Zappa Achille, Splendiani Andrea, Romano Paolo   +2 more
doaj   +1 more source

Translophagy—A potential link between autophagy impairment and translational errors

FEBS Letters, EarlyView.
Neurodegenerative diseases are characterised by the accumulation of abnormal proteins and protein aggregates, but their origin often remains unknown. We propose that selective autophagy removes damaged protein‐making machinery, preventing errors during protein synthesis.
Mykola V. Korolchuk, Gailing Ma, Robin Antrobus, Diego Manni, Marco Trevisan‐Herraz, Mustafa Khwaja, Max Brown, Gavin D. Richardson, Masaaki Komatsu, Daniel Erskine, Viktor I. Korolchuk, Tetsushi Kataura   +11 more
wiley   +1 more source

Linked Open Data for Environmental Protection in Smart Regions – from INSPIRE Data to Semantic WEB Data

This paper introduces with the specific objectives and initial results of the recently started project SmartOpenData - “Linked Open Data for Environmental Protection in Smart Regions” (SmOD project) that is supported by Seventh Framework ENV.2013.6.5-3 ...
Alberts, Māris, Čerba, Otakar, Kafka, Stepan, Archer, Phil, Charvat, Karel, Bruns, Peteris, Barvika, Sarmīte   +6 more
core  

Construction, Deployment, and Usage of the Human Reference Atlas Knowledge Graph

Scientific Data
The Human Reference Atlas (HRA) for the healthy, adult body is being developed by a team of international, interdisciplinary experts across 25+ consortia.
Andreas Bueckle, Bruce W. Herr, Josef Hardi, Ellen M. Quardokus, Mark A. Musen, Katy Börner   +5 more
doaj   +1 more source

Functional comparison of EncB and EncC cargo proteins in iron storage within the Myxococcus xanthus encapsulin

FEBS Letters, EarlyView.
Encapsulins are protein nanocompartments that play an important role in iron storage. In the Myxococcus xanthus encapsulin system, two cargo proteins called EncB and EncC contribute to iron mineralization. Here, we show that EncB and EncC generate iron‐containing minerals with distinct chemical compositions, suggesting that the composition of stored ...
Harry B. McDowell, Thomas Walther, Egbert Hoiczyk   +2 more
wiley   +1 more source

Structural and biochemical analysis of a B12 superbinder

FEBS Letters, EarlyView.
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices, Jacob J. Whittaker, Dirk J. Slotboom, Albert Guskov   +3 more
wiley   +1 more source

Structures of mycobacterial 3‐methylcrotonyl‐CoA carboxylase reveal carrier‐domain translocation between catalytic sites

FEBS Letters, EarlyView.
Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav, Bogdan I. Florea, Sebastian Geibel   +2 more
wiley   +1 more source