Results 11 to 20 of about 276,768 (304)

Simple Procedure for Creation of In-Frame Deletion Mutations Throughout an Open Reading Frame

BioTechniques, 1996
A general method is presented for randomly mutagenizing open reading frames (ORF) to generate in-frame deletions and insertions. Theprotocol requires expression of the ORF of interest as a hybrid ORF-β-galactosidase fusion protein.
John H. Ludes-Meyers, Van G. Wilson
doaj   +2 more sources

Evidence for Translational Repression of the SOCS-1 Major Open Reading Frame by an Upstream Open Reading Frame

Biochemical and Biophysical Research Communications, 2000
The suppressor of cytokine signalling 1 protein (SOCS-1) belongs to a novel family of cytokine inducible factors which function as inhibitors of the JAK/STAT pathway. While SOCS-1 previously has been described as a single-exon gene, here we present evidence for an additional 5' exon, separated by a 509 bp intron from exon 2.
Schluter, G., Boinska, D., Nieman-Seyde, S. C.   +2 more
openaire   +4 more sources

A novel vector for positive selection of inserts harboring an open reading frame by translational coupling

BioTechniques, 2007
We have developed a novel vector, pTCS, as a tool for efficient selection of open reading frame (ORF)-containing inserts. In pTCS clones containing an insert with an ORF, a downstream marker gene (immE3, conferring resistance to colicin) is activated via
Sumiko Ohashi-Kunihiro, Masafumi Yohda, Haruhiko Masaki, Masayuki Machida   +3 more
doaj   +2 more sources

A Coding Sequence-Embedded Principle Governs Translational Reading Frame Fidelity

Research, 2018
Upon initiation at a start codon, the ribosome must maintain the correct reading frame for hundreds of codons in order to produce functional proteins. While some sequence elements are able to trigger programmed ribosomal frameshifting (PRF), very little ...
Ji Wan, Xiangwei Gao, Yuanhui Mao, Xingqian Zhang, Shu-Bing Qian   +4 more
doaj   +2 more sources

An upstream open reading frame regulates LST1 expression during monocyte differentiation. [PDF]

PLoS ONE, 2014
The regulation of gene expression depends on the interplay of multiple factors at the transcriptional and translational level. Upstream open reading frames (uORFs) play an important role as translational repressors of main ORFs and their presence or ...
Christian Schiller, Carina Nowak, Kalliope N Diakopoulos, Ulrich H Weidle, Elisabeth H Weiss   +4 more
doaj   +1 more source

Mitochondrial-Derived Peptides in Diabetes and Its Complications

Frontiers in Endocrinology, 2022
The changes of mitochondrial function are closely related to diabetes and its complications. Here we describe the effects of mitochondrial-derived peptides (MDPs), short peptides formed by transcription and translation of the open reading frame site in ...
Ying Wu, Liankun Sun, Zhoudao Zhuang, Xiaoqing Hu, Delu Dong   +4 more
doaj   +1 more source

The random nature of genome architecture: predicting open reading frame distributions. [PDF]

PLoS ONE, 2009
BackgroundA better understanding of the size and abundance of open reading frames (ORFS) in whole genomes may shed light on the factors that control genome complexity.
Michael W McCoy, Andrew P Allen, James F Gillooly   +2 more
doaj   +1 more source

A Novel Mutation in the Upstream Open Reading Frame of the CDKN1B Gene Causes a MEN4 Phenotype [PDF]

, 2013
PubMed ID: 23555276This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are ...
OCCHI, GIANLUCA, Pellegata, NS, Pardi E, Giampaolo Trivellin, Franco Mantero (392960), Cetani, F., Carla Scaroni, Sidarovich V, Carla Scaroni (392961), CIATO, DENIS, Sidarovich, V, Ciato, D., Franco Mantero, Denis Ciato (392953), Regazzo, D, Ferasin, S, Viktoryia Sidarovich, Quattrone, A., Giampaolo Trivellin (106006), Pardi, E, MANTERO, FRANCO, Francesca Boaretto (392952), Bobisse S, Pellegata NS, Denis Ciato, SCARONI, CARLA, Korbonits M, Márta Korbonits (59514), Opocher, G., Sara Bobisse (392954), Regazzo, D., Gianluca Occhi (38330), Daniela Regazzo, Viktoryia Sidarovich (392959), Francesca Boaretto, Daniela Regazzo (392951), REGAZZO, DANIELA, Korbonits, M., Filomena Cetani, Scaroni, C, Ciato, D, Natalia S Pellegata, Mantero, F, Elena Pardi (392957), Pellegata, N.S., Giuseppe Opocher (260071), Giuseppe Opocher, Scaroni, C., Alessandro Quattrone (220189), Cetani F, Bobisse, S, Boaretto, F, Mantero, F., Trivellin G, Quattrone, A, Elena Pardi, Márta Korbonits, Sergio Ferasin, Trivellin, G, Sidarovich, V., Filomena Cetani (392956), BOARETTO, FRANCESCA, Occhi, G., Natalia S. Pellegata (392958), Bobisse, S., Sara Bobisse, Trivellin, G., Boaretto, F., Sergio Ferasin (392955), Gianluca Occhi, Alessandro Quattrone, OPOCHER, GIUSEPPE, FERASIN, SERGIO, Opocher, G, Ferasin, S., Korbonits, M, Pardi, E., Occhi, G, Quattrone A, Cetani, F   +79 more
core   +1 more source

Inhibition of Host Gene Expression by KSHV: Sabotaging mRNA Stability and Nuclear Export

Frontiers in Cellular and Infection Microbiology, 2021
Viruses are known for their ability to alter host gene expression. Kaposi sarcoma-associated herpesvirus has two proteins that obstruct host gene expression.
Carissa Ikka Pardamean, Ting-Ting Wu
doaj   +1 more source

Selecting Open Reading Frames From DNA [PDF]

Genome Research, 2003
We describe a method to select DNA encoding functional open reading frames (ORFs) from noncoding DNA within the context of a specific vector. Phage display has been used as an example, but any system requiring DNA encoding protein fragments, for example, the yeast two-hybrid system, could be used.
Zacchi P., Sblattero D., FLORIAN, FIORELLA, MARZARI, ROBERTO, Bradbury A.   +4 more
openaire   +3 more sources