Results 21 to 30 of about 1,287,785 (343)
Selecting Open Reading Frames From DNA [PDF]
Genome Research, 2003 We describe a method to select DNA encoding functional open reading frames (ORFs) from noncoding DNA within the context of a specific vector. Phage display has been used as an example, but any system requiring DNA encoding protein fragments, for example, the yeast two-hybrid system, could be used.
Zacchi P. +4 more
openaire +3 more sources
Standardized annotation of translated open reading frames [PDF]
Nature Biotechnology, 2022 Ribosome profiling (Ribo-seq) has extended our understanding of the translational ‘vocabulary’ of the human genome, uncovering thousands of open reading frames (ORFs) within long noncoding RNAs (lncRNAs) and presumed untranslated regions (UTRs) of protein-coding genes.
Jonathan M. Mudge +34 more
openaire +6 more sources
The random nature of genome architecture: predicting open reading frame distributions. [PDF]
PLoS ONE, 2009 BackgroundA better understanding of the size and abundance of open reading frames (ORFS) in whole genomes may shed light on the factors that control genome complexity.
Michael W McCoy +2 more
doaj +1 more source
Inhibition of Host Gene Expression by KSHV: Sabotaging mRNA Stability and Nuclear Export
Frontiers in Cellular and Infection Microbiology, 2021 Viruses are known for their ability to alter host gene expression. Kaposi sarcoma-associated herpesvirus has two proteins that obstruct host gene expression.
Carissa Ikka Pardamean, Ting-Ting Wu
doaj +1 more source
Transduplication resulted in the incorporation of two protein-coding sequences into the Turmoil-1 transposable element of C. elegans [PDF]
, 2008 Transposable elements may acquire unrelated gene fragments into their sequences in a process called transduplication. Transduplication of protein-coding genes is common in plants, but is unknown of in animals.
Ast, Gil +4 more
core +4 more sources
Novel exon of mammalian ADAR2 extends open reading frame.
PLoS ONE, 2009 BackgroundThe post-transcriptional processing of pre-mRNAs by RNA editing contributes significantly to the complexity of the mammalian transcriptome. RNA editing by site-selective A-to-I modification also regulates protein function through recoding of ...
Stefan Maas, Willemijn M Gommans
doaj +1 more source
The Hepatitis E Virus Open Reading Frame 2 Protein: Beyond Viral Capsid
Frontiers in Microbiology, 2021 Hepatitis E virus (HEV) is a zoonotic pathogen causing hepatitis in both human and animal hosts, which is responsible for acute hepatitis E outbreaks worldwide. The 7.2 kb genome of the HEV encodes three well-defined open reading frames (ORFs), where the
Zhaobin Zhou +6 more
doaj +1 more source
Simple Procedure for Creation of In-Frame Deletion Mutations Throughout an Open Reading Frame
BioTechniques, 1996 A general method is presented for randomly mutagenizing open reading frames (ORF) to generate in-frame deletions and insertions. Theprotocol requires expression of the ORF of interest as a hybrid ORF-β-galactosidase fusion protein.
John H. Ludes-Meyers, Van G. Wilson
doaj +1 more source
Identification of a protein encoded in the EB-viral open reading frame BMRF2 [PDF]
, 1992 Using monospecific rabbit sera against a peptide derived from a potential antigenic region of the Epstein-Barr viral amino acid sequence encoded in the open reading frame BMRF2 we could identify a protein-complex of 53/55 kDa in chemically induced B95-8,
A. v. Brunn +37 more
core +2 more sources
Nature Communications, 2019 The majority of enterovirus proteins are encoded as polyprotein in one open reading frame. Here, Guo et al. characterize a second open reading frame in human enteroviruses and show that it is important for viral particle release, particularly in ...
Haoran Guo +9 more
doaj +1 more source