Simple Procedure for Creation of In-Frame Deletion Mutations Throughout an Open Reading Frame
BioTechniques, 1996 A general method is presented for randomly mutagenizing open reading frames (ORF) to generate in-frame deletions and insertions. Theprotocol requires expression of the ORF of interest as a hybrid ORF-β-galactosidase fusion protein.
John H. Ludes-Meyers, Van G. Wilson
doaj +4 more sources
HAltORF: a database of predicted out-of-frame alternative open reading frames in human [PDF]
Database, 2012 Human alternative open reading frames (HAltORF) is a publicly available and searchable online database referencing putative products of out-of-frame alternative translation initiation (ATI) in human mRNAs.
Benoît Vanderperre +2 more
semanticscholar +3 more sources
MicroRNA-34a modulates MDM4 expression via a target site in the open reading frame. [PDF]
PLoS ONE, 2012 BACKGROUND: MDM4, also called MDMX or HDMX in humans, is an important negative regulator of the p53 tumor suppressor. MDM4 is overexpressed in about 17% of all cancers and more frequently in some types, such as colon cancer or retinoblastoma.
Pooja Mandke +5 more
doaj +2 more sources
Nucleotide sequence of the human c-myc locus: provocative open reading frame within the first exon. [PDF]
EMBO Journal, 1984 Claude Gazin +6 more
openalex +2 more sources
Open reading frame cloning: identification, cloning, and expression of open reading frame DNA. [PDF]
Proceedings of the National Academy of Sciences, 1982 A plasmid was constructed that facilitates the cloning and expression of open reading frame DNA. A DNA fragment containing a bacterial promoter and the amino terminus of the cI gene of bacteriophage lambda was fused to an amino-terminally deleted version of the lacZ gene.
Hildur V. Colot +3 more
openaire +3 more sources
Conserved Upstream Open Reading Frame Nascent Peptides that Control Translation.
Annual Review of Genetics, 2020 Cells utilize transcriptional and posttranscriptional mechanisms to alter gene expression in response to environmental cues. Gene-specific controls, including changing the translation of specific messenger RNAs (mRNAs), provide a rapid means to respond ...
T. Dever, I. Ivanov, M. Sachs
semanticscholar +1 more source
AlterORF: a database of alternate open reading frames [PDF]
Nucleic Acids Research, 2007 AlterORF is a searchable database that contains information regarding alternate open reading frames (ORFs) for over 1.5 million genes in 481 prokaryotic genomes. The objective of the database is to provide a platform for improving genome annotation and to serve as an aid for the identification of prokaryotic genes that potentially encode proteins in ...
Pedroso, Inti +6 more
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Selecting Open Reading Frames From DNA [PDF]
Genome Research, 2003 We describe a method to select DNA encoding functional open reading frames (ORFs) from noncoding DNA within the context of a specific vector. Phage display has been used as an example, but any system requiring DNA encoding protein fragments, for example, the yeast two-hybrid system, could be used.
Zacchi P. +4 more
openaire +4 more sources
Mitochondrial-Derived Peptides in Diabetes and Its Complications
Frontiers in Endocrinology, 2022 The changes of mitochondrial function are closely related to diabetes and its complications. Here we describe the effects of mitochondrial-derived peptides (MDPs), short peptides formed by transcription and translation of the open reading frame site in ...
Ying Wu +4 more
doaj +1 more source
The random nature of genome architecture: predicting open reading frame distributions. [PDF]
PLoS ONE, 2009 BackgroundA better understanding of the size and abundance of open reading frames (ORFS) in whole genomes may shed light on the factors that control genome complexity.
Michael W McCoy +2 more
doaj +1 more source