Results 31 to 40 of about 780,027 (226)
Identifying Small Open Reading Frames in Prokaryotes with Ribosome Profiling
Journal of Bacteriology, 2021 Small proteins encoded by open reading frames (ORFs) shorter than 50 codons (small ORFs [sORFs]) are often overlooked by annotation engines and are difficult to characterize using traditional biochemical techniques.
N. Vázquez-Laslop +3 more
semanticscholar +1 more source
Pervasive functional translation of noncanonical human open reading frames
Science, 2020 Expanding the human proteome Using mass spectrometry, ribosome profiling, and several CRISPR-based screens, Chen et al. identified hundreds of previously uncharacterized functional micropeptides in the human genome (see the Perspective by Wei and Guo ...
Jin Chen +10 more
semanticscholar +1 more source
OpenVar: functional annotation of variants in non-canonical open reading frames
Cell & Bioscience, 2022 Background Recent technological advances have revealed thousands of functional open reading frames (ORF) that have eluded reference genome annotations. These overlooked ORFs are found throughout the genome, in any reading frame of transcripts, mature or ...
Marie A. Brunet +2 more
doaj +1 more source
Selecting Open Reading Frames From DNA [PDF]
Genome Research, 2003 We describe a method to select DNA encoding functional open reading frames (ORFs) from noncoding DNA within the context of a specific vector. Phage display has been used as an example, but any system requiring DNA encoding protein fragments, for example, the yeast two-hybrid system, could be used.
Zacchi P. +4 more
openaire +3 more sources
Translation of small downstream ORFs enhances translation of canonical main open reading frames
EMBO Journal, 2020 In addition to canonical open reading frames (ORFs), thousands of translated small ORFs (containing less than 100 codons) have been identified in untranslated mRNA regions (UTRs) across eukaryotes.
Qiushuang Wu +5 more
semanticscholar +1 more source
BMC Genomics, 2006 Background Approximately 40% of mammalian mRNA sequences contain AUG trinucleotides upstream of the main coding sequence, with a quarter of these AUGs demarcating open reading frames of 20 or more codons.
Wang Xue-Qing +2 more
doaj +1 more source
Experimental Cell Research, 2020 Mitochondrial-derived peptides (MDPs) are a novel class of bioactive microproteins that modify cell metabolism. To date, eight MDPs have been characterized (e.g., humanin, MOTS-c, SHLPs1-6) and attenuate disease pathology, including Alzheimer's disease ...
Brendan Miller +7 more
semanticscholar +1 more source
The mitochondrial genome of Cavia aperea
Mitochondrial DNA. Part B. Resources, 2020 Cavia aperea is a wild guinea pig found throughout South America. The previously published mitochondrial sequence for C. aperea was highly divergent from the C. porcellus sequence and contained stop codons within open reading frames.
Azizia Wahedi +3 more
doaj +1 more source
RiboNT: A Noise-Tolerant Predictor of Open Reading Frames from Ribosome-Protected Footprints
Life, 2021 Ribo-seq, also known as ribosome profiling, refers to the sequencing of ribosome-protected mRNA fragments (RPFs). This technique has greatly advanced our understanding of translation and facilitated the identification of novel open reading frames (ORFs ...
Bo Song, Mengyun Jiang, Lei Gao
doaj +1 more source
De Novo Genome Sequence of "Candidatus Liberibacter solanacearum" from a Single Potato Psyllid in California. [PDF]
, 2015 The draft genome sequence of "Candidatus Liberibacter solanacearum" strain RSTM from a potato psyllid (Bactericera cockerelli) in California is reported here. The RSTM strain has a genome size of 1,286,787 bp, a G+C content of 35.1%, 1,211 predicted open
Chen, J +6 more
core +1 more source