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Characterization and Transcriptional Regulation of the 2-Ketogluconate Utilization Operon in Pseudomonas plecoglossicida [PDF]

open access: yesMicroorganisms
Pseudomonas plecoglossicida JUIM01 is an industrial 2-keto-d-gluconate (2KGA)-producing strain. However, its regulation mechanism of 2KGA metabolism remains to be clarified. Among other reported Pseudomonas species, the 2-ketogluconate utilization operon
Lei Sun   +8 more
doaj   +2 more sources

AcrDB update: Predicted 3D structures of anti-CRISPRs in human gut viromes. [PDF]

open access: yesProtein Sci
Abstract Anti‐CRISPR (Acr) proteins play a key role in phage‐host interactions and hold great promise for advancing genome‐editing technologies. However, finding new Acrs has been challenging due to their low sequence similarity. Recent advances in protein structure prediction have opened new pathways for Acr discovery by using 3D structure similarity.
Khatri M   +4 more
europepmc   +2 more sources

Mutational Analysis Supports Three-Hairpin Model of Attenuator for Transcription Regulation of ilvBNC Operon in Corynebacterium glutamicum [PDF]

open access: yesMicroorganisms
The ilvBNC operon in Corynebacterium glutamicum encodes key enzymes for the biosynthesis of branched-chain amino acids (L-isoleucine, L-leucine, and L-valine).
Ludmila E. Ryabchenko   +8 more
doaj   +2 more sources

Multiplexed mRNA assembly into ribonucleoprotein particles plays an operon-like role in the control of yeast cell physiology

open access: yeseLife, 2021
Prokaryotes utilize polycistronic messages (operons) to co-translate proteins involved in the same biological processes. Whether eukaryotes achieve similar regulation by selectively assembling and translating monocistronic messages derived from different
Rohini R Nair   +7 more
doaj   +1 more source

Reporter Gene-Based qRT-PCR Assay for Rho-Dependent Termination In Vivo

open access: yesCells, 2023
In bacteria, the Rho protein mediates Rho-dependent termination (RDT) by identifying a non-specific cytosine-rich Rho utilization site on the newly synthesized RNA. As a result of RDT, downstream RNA transcription is reduced.
Monford Paul Abishek N   +3 more
doaj   +1 more source

Stoichiometry of the Gene Products From the Tetrachloroethene Reductive Dehalogenase Operon pceABCT

open access: yesFrontiers in Microbiology, 2022
Organohalide respiration (OHR) is a bacterial anaerobic process that uses halogenated compounds, e.g., tetrachloroethene (PCE), as terminal electron acceptors.
Lorenzo Cimmino   +3 more
doaj   +1 more source

Diel gene expression improves software prediction of cyanobacterial operons [PDF]

open access: yesPeerJ, 2022
Cyanobacteria are important participants in global biogeochemical process, but their metabolic processes and genomic functions are incompletely understood.
Philip Heller
doaj   +2 more sources

Internal Promoters and Their Effects on the Transcription of Operon Genes for Epothilone Production in Myxococcus xanthus

open access: yesFrontiers in Bioengineering and Biotechnology, 2021
The biosynthetic genes for secondary metabolites are often clustered into giant operons with no transcription terminator before the end. The long transcripts are frangible and the transcription efficiency declines along with the process.
Ye Wang   +5 more
doaj   +1 more source

Evaluation of Biofilm Formation and Presence of Genes in Clinical Isolates [PDF]

open access: yesOsong Public Health and Research Perspectives, 2018
Objectives Biofilm formation is one of the important features of Staphylococcus epidermidis, particularly in nosocomial infections. We aimed to investigate the biofilm production by phenotypic methods and the presence of ica genes in S epidermidis ...
Maryam Kord   +5 more
doaj   +1 more source

FADU: a Quantification Tool for Prokaryotic Transcriptomic Analyses

open access: yesmSystems, 2021
Quantification tools for RNA sequencing (RNA-Seq) analyses are often designed and tested using human transcriptomics data sets, in which full-length transcript sequences are well annotated.
Matthew Chung   +10 more
doaj   +1 more source

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