Results 101 to 110 of about 11,787,568 (310)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

open access: yesFEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz   +6 more
wiley   +1 more source

Design of a Society of Automotive Engineers Regular Curved Retroreflector for Enhancing Optical Efficiency and Working Area

open access: yesCrystals, 2018
A highly efficient regular curved retroreflector is proposed to meet the requirements of the US Society of Automotive Engineers (SAE) regulations. It is demonstrated that 28% higher retroreflection efficiency and 33% more working area can be accomplished
Lanh-Thanh Le   +4 more
doaj   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

open access: yesFEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj   +3 more
wiley   +1 more source

Development of an Imaging Spectrometer with a High Signal-to-Noise Ratio Based on High Energy Transmission Efficiency for Soil Organic Matter Detection

open access: yesSensors
Hyperspectral detection of the change rate of organic matter content in agricultural remote sensing requires a high signal-to-noise ratio (SNR). However, due to the large number and efficiency limitation of the components, it is difficult to improve the ...
Jize Fan   +7 more
doaj   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

open access: yesFEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

A Single-Element Plane Grating Monochromator

open access: yesPhotonics, 2016
Concerted rotations of a self-focused varied line-space diffraction grating about its groove axis and surface normal define a new geometric class of monochromator.
Michael C. Hettrick
doaj   +1 more source

Design of infrared diffractive telescope imaging optical systems

open access: yes, 2015
Diffractive telescope is an updated imaging technology, it differs from conventional refractive and reflective imaging system, which is based on the principle of diffraction image. It has great potential for developing the larger aperture and lightweight
Kang, Fuzeng   +11 more
core   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Measurements On Passive Tunable Optical Filters For DWDM [PDF]

open access: yes, 2002
WFOPC 2002 IEEE/LEOS Workshop on Fibre and Optical Passive Components, University of Glasgow, Scotland, on 5th-6th June 2002.Measurements on a passive optical fibre tunable filter are presented.
Sánchez-Pena, José Manuel   +3 more
core   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

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