Results 1 to 10 of about 1,291 (163)

Quantitative Caveats of Standard Immunohistochemical Procedures: Implications for Optical Disector–based Designs [PDF]

Journal of Histochemistry and Cytochemistry, 2010
Immunohistochemistry is a ubiquitous technique in histology. Often, the goal of such studies is the quantification of some parameter associated with a particular antigen. When used correctly, the optical disector offers a statistically relevant approach to achieve this goal without bias from cell size, shape, or orientation.
Robert J Sutherland
exaly   +5 more sources

Morphometric Analysis of Infant and Adult Medullary Nuclei Through Optical Disector Method [PDF]

Anatomical Record, 2009
AbstractIn the literature, comprehensive and comparative morphometric studies of infant and adult medullary nuclei performed with unbiased methods are still lacking. In this study, the unbiased quantitative method of the optical disector was applied to analyze neuronal densities, nuclear volumes, and total neuron numbers of the hypoglossal nucleus (XII)
Andrea Porzionato, Veronica Macchi, Anna Parenti   +2 more
exaly   +6 more sources

Curcumin mitigates the sleep-deprivation impacts on rat hypothalamic paraventricular nucleus [PDF]

IBRO Neuroscience Reports, 2023
Introduction: The paraventricular nucleus of the hypothalamus (PVH) is an important efferent system that relays the circadian rhythm of sleep and stress information to the periphery.
Mahboobeh Erfanizadeh, Ali Noorafshan, Mohammad Reza Namavar, Saied Karbalay-Doust, Tahereh Talaei-Khozani   +4 more
doaj   +2 more sources

Absolute Number of Thalamic Parafascicular and Striatal Cholinergic Neurons, and the Three-Dimensional Spatial Array of Striatal Cholinergic Neurons, in the Sprague-Dawley Rat. [PDF]

J Comp Neurol
The absolute numbers of thalamic parafascicular (PFN) neurons and striatal cholinergic interneurons (CIN) were estimated in rats using unbiased stereological methods. Spatial statistical analysis of the three‐dimensional distribution revealed that the large, mostly CIN were randomly positioned with no evidence of clustering.
Zhang R, Wickens JR, Carrasco A, Oorschot DE.   +3 more
europepmc   +2 more sources

Optical disector counting in cryosections and vibratome sections underestimates particle numbers: effects of tissue quality. [PDF]

Microsc Res Tech, 2008
AbstractOptical disector counting is currently applied most often to cryosections, followed in frequency by resin‐embedded tissues, paraffin, and vibratome sections. The preservation quality of these embedding options differs considerably; yet, the effect of tissue morphology on numerical estimates is unknown.
Ward TS, Rosen GD, von Bartheld CS.
europepmc   +4 more sources

High vulnerability of medial prefrontal pyramidal neurons in post-stroke, vascular, Alzheimer's disease, and aging-related dementias. [PDF]

Alzheimers Dement
Abstract INTRODUCTION The medial prefrontal cortex (mPFC) is critical for executive function, behavioral inhibition, and memory. Its high vulnerability to dementia, compared to other prefrontal regions, remains unclear. METHODS We analyzed post mortem brain tissue from 118 older subjects, including post‐stroke survivors, Alzheimer's disease; vascular ...
Jobson DD, Hase Y, Walker L, Polvikoski T, Khundakar AA, Allan L, Kalaria RN.   +6 more
europepmc   +2 more sources

Counting glomeruli in human kidney specimens using ex vivo MRI without contrast agents. [PDF]

Magn Reson Med
Abstract Purpose The total number of glomeruli is an important indicator of kidney function and susceptibility to disease. Glomeruli, however, are difficult to image using MRI without contrast enhancement. For this reason, previous studies have used cationic ferritin as a contrast agent to detect glomeruli in ex vivo and in vivo perfused kidneys.
Kurniawan ND, Amar AJ, Cullen-McEwen LA, Combes AN, Hayatudin R, Kassianos AJ, Du J, Gazzard SE, Healy HG, Hoy WE, Bertram JF, Reutens DC.   +11 more
europepmc   +2 more sources

Total neuron counts in vulnerable and resilient entorhinal subregions [PDF]

Alzheimers Dement
Abstract Background Neuron loss exceeds tau tangle formation (Gomez‐Isla et al. 1997) and p‐tau vulnerability differs by layer and subfield in entorhinal cortex (Llamas‐Rodriguez et al., 2022). Some subfields succumb to tau early and other subfields later in Alzheimer’s disease.
Rosenblum E, Augustinack J.
europepmc   +2 more sources

Stereological and biochemical effects of thymoquinone on ovarian tissue toxicity induced by silver nanoparticles in NMRI mice: An experimental study [PDF]

International Journal of Reproductive BioMedicine
Background: The toxicity of silver nanoparticles (AgNPs) has been proven in the female reproductive system. Thymoquinone (TQ) is a natural antioxidant and bioactive component of Nigella sativa. Objective: We evaluated the efficacy of TQ on ovarian tissue
Seyed Mohammad Ali Shariatzadeh, Fatemeh Salmani, Hossein Moghanlo, Monireh Mahmoodi   +3 more
doaj   +2 more sources

Wharton's Jelly Mesenchymal Stem Cell Conditioned Medium Ameliorates Diabetes-Induced Testicular Damage and Sperm Abnormalities by Mitigating Oxidative Stress, Apoptosis, and Inflammation. [PDF]

Stem Cells Int
Diabetes leads to testicular damage and infertility. Mesenchymal stem cells and their secretory trophic factors have shown potential as regenerative therapies for diabetes and its associated complications. This study examined the effects of conditioned medium derived from Wharton’s jelly mesenchymal stem cells (WJMSCs‐CM) on sperm parameters ...
Sargazi M, Karbalaei N, Karbalay-Doust S, Keshtgar S, Aghaei Z.   +4 more
europepmc   +2 more sources